Phospho-FUNDC1 (Ser17) Antibody – Research Use Only

Phospho-FUNDC1 (Ser17) Antibody is a site-specific polyclonal antibody that recognizes FUNDC1 phosphorylated at serine 17. Supplied as a 50 µL solution (Catalog #B2017874), this antibody is a valuable research tool for studying mitophagy regulation, as phosphorylation of FUNDC1 at Ser17 modulates its interaction with LC3 and plays a key role in hypoxia-induced mitophagy and mitochondrial quality control.

Scientific Overview

FUNDC1 is an outer mitochondrial membrane protein that acts as a receptor for selective autophagy (mitophagy). Phosphorylation at Ser17 (p-FUNDC1 Ser17) is a key regulatory modification that enhances the interaction between FUNDC1 and LC3, thereby promoting mitophagosome formation, especially under hypoxic conditions or during mitochondrial stress. This phospho-specific antibody enables researchers to detect and quantify the activated/phosphorylated form of FUNDC1, facilitating studies on mitochondrial quality control, cellular adaptation to hypoxia, and the role of mitophagy in disease states such as neurodegeneration, ischemia, and cancer.

This Phospho-FUNDC1 (Ser17) Antibody is suitable for:

• Detection of Ser17-phosphorylated FUNDC1 by Western blot
• Immunohistochemistry and immunofluorescence analysis of mitophagy
• Studies of hypoxia-induced mitophagy and mitochondrial dynamics
• Investigation of FUNDC1 regulation in metabolic stress and disease models

Usage & Handling Guidance

Store at -20 °C. Avoid repeated freeze–thaw cycles by preparing single-use aliquots. For Western blotting, typical dilutions range from 1:500 to 1:2000. For IHC/IF, optimize starting from 1:100–1:500. Centrifuge briefly before use to remove any aggregates.

• Recommended applications: Western blot, IHC, and IF
• Working dilution: Optimize empirically (commonly 1:500–1:2000 for WB; 1:100–1:500 for IHC/IF)
• Stability: Stable when stored frozen; aliquot to prevent repeated freeze–thaw
• Handling: Use in a clean environment; validate specificity with appropriate controls (e.g., phosphatase treatment)

What You Get

• 50 μL Phospho-FUNDC1 (Ser17) Antibody solution
• Site-specific antibody recognizing FUNDC1 phosphorylated at serine 17
• High-purity biotechnology-grade reagent suitable for sensitive detection methods
• Convenient volume for multiple experiments and protocol optimization
• For research use only (RUO)

Why Researchers Choose It

• Specific detection of the functionally important Ser17-phosphorylated form of FUNDC1
• Essential tool for studying mitophagy, hypoxia response, and mitochondrial quality control
• High sensitivity and low background in Western blot, IHC, and immunofluorescence
• Reliable performance in cell biology and disease model research
• Biotechnology-grade quality ensures reproducibility

Frequently Asked Questions (FAQ)

• What does this antibody specifically detect?
  It recognizes FUNDC1 only when phosphorylated at serine 17 (p-FUNDC1 Ser17).
• What is the significance of Ser17 phosphorylation on FUNDC1?
  Phosphorylation at Ser17 enhances FUNDC1 binding to LC3, promoting mitophagy under hypoxic or stress conditions.
• What dilution should I use for Western blot?
  Start with 1:500–1:2000 and optimize for your specific sample and detection system.
• Can this antibody be used for immunofluorescence?
  Yes. It is suitable for IF and IHC after proper optimization and validation with controls.
• How should I store the antibody?
  Store at -20 °C and avoid repeated freeze–thaw cycles by making aliquots.

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References

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