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Mouse IgG Purified (Immunoglobulins G) Antibodies Molecular Depot
Mouse IgG Purified (Immunoglobulins G) Antibodies Molecular Depot
Mouse IgG Purified (Immunoglobulins G) Antibodies Molecular Depot
Mouse IgG Purified (Immunoglobulins G) Antibodies Molecular Depot
Mouse IgG Purified (Immunoglobulins G) Antibodies Molecular Depot
Mouse IgG Purified (Immunoglobulins G) Antibodies Molecular Depot

Mouse IgG Purified (Immunoglobulins G)

$452.00

    Catalog #: A2010033 (10 mg)

    Mouse IgG purified on protein A Sepharose can be used as non specific blockers in immunossays, ELISA, WB and immunohistochemistry. Bulk pricing and biotinylation services are available.

    Products are for in vitro research use only (RUO).

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Mouse IgG Purified (Immunoglobulins G)

Catalog number: A2010033
Lot number: Batch Dependent
Expiration date: Batch Dependent
Package Size: 10 mg and bulk
Other names: Mouse immunoglobulins G
Supplied as: Solution
Concentration: 10-12 mg/mL
Clonality: Polyclonal
Host: Mouse
Specificity: n/a
Cross-reaction: n/a
Purification: Chromatography on protein A Sepharose
Immunogen n/a
Preservative: 0.05% sodium azide
Storage: 2-8°C.

 

Mouse IgG Purified is a high-quality polyclonal immunoglobulin G preparation isolated from mouse serum and purified via protein A Sepharose chromatography. Supplied as a liquid solution at a concentration of 10–12 mg/mL, it is ideal for use as a non-specific blocking agent in immunoassays, including ELISA, Western blotting, and immunohistochemistry.

This reagent is particularly useful for reducing background noise and preventing non-specific binding in assays involving mouse-derived antibodies. Its high purity and consistent performance make it a reliable component in assay optimization, antibody labeling, and control experiments. Bulk quantities and biotinylation services are available to support high-throughput workflows and custom assay development.

Key benefits:

  • Purified via protein A Sepharose for >95% purity

  • Effective as a non-specific blocker in immunoassays

  • Supplied in liquid format for immediate use

  • Available in bulk for scalable research needs

  • Compatible with ELISA, WB, IHC, and more

Products are for in vitro research use only (RUO).


References

  • Highly reduced binding to high and low affinity mouse Fc gamma receptors by L234A/L235A and N297A Fc mutations engineered into mouse IgG2a. Arduin E, Arora S, Bamert PR, Kuiper T, Popp S, Geisse S, Grau R, Calzascia T, Zenke G, Kovarik J. Mol Immunol. 2015 Feb;63(2):456-63.
  • A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas. Liu H, White J, Crawford F, Jin N, Ju X, Liu K, Jiang C, Marrack P, Zhang G, Kappler JW. PLoS One. 2015 Aug 28;10(8):e0136613.
  • Optimization of biotin labeling of antibodies using mouse IgG and goat anti-mouse IgG-conjugated fluorescent beads and their application as capture probes on protein chip.Lee JH, Choi HK, Chang JH. J Immunol Methods. 2010 Oct 31;362(1-2):38-42.
  • Generating and Purifying Fab Fragments from Human and Mouse IgG Using the Bacterial Enzymes IdeS, SpeB and Kgp. Sjögren J, Andersson L, Mejàre M, Olsson F. Methods Mol Biol. 2017;1535:319-329. doi: 10.1007/978-1-4939-6673-8_21.
  • Analysis of the linear epitope for Fc-binding on the mouse IgG Fc receptor (moFcγRI) by synthetic peptide. Wang FY, Guo JQ, Zhang GP. Genet Mol Res. 2014 Jun 18;13(2):4647-53.
  • Detection of injected fluorescence-conjugated IgG in living mouse organs using “in vivo cryotechnique” with freeze-substitution. Terada N, Ohno N, Li Z, Fujii Y, Baba T, Ohno S. Microsc Res Tech. 2005 Mar 1;66(4):173-8.
  • IgG heterophile antibody causes false positivity for CA19-9, which is overcome with bovine immunoglobulin. Miura H, Kitano M, Yoneyama A, Kuwahara A, Moriyama K, Kitajima S. Rinsho Byori. 2005 Dec;53(12):1103-8.

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    Copy of Technical Specifications

    FeatureDetails
    Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
    Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
    Nosepiece Quintuple
    Quintuple LWD Planachromat Phase 10x, 20x
    Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
    Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
    IlluminationKoehler without iris, with phase slider, 3W LED
    WarrantyLIMITED LIFETIME WARRANTY

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