Iron Oxide Nanoparticles in Water, 10–30 nm – Catalog #B2026057

Iron Oxide Nanoparticles in Water (Catalog #B2026057) consist of magnetic iron oxide particles (typically magnetite, Fe₃O₄) with a size range of 10–30 nm, supplied as a ready-to-use aqueous suspension. The hydrophilic surface enables stable dispersion in water and aqueous buffers, making this product ideal for biological applications including magnetic cell separation, protein labeling, and diagnostic assays. Supplied as 2 mL aqueous suspension, this product offers convenient handling without the need for solvent exchange.

Scientific Overview

Iron oxide nanoparticles are among the most versatile nanomaterials in biomedical research because they combine strong magnetic properties with low toxicity and surface modifiability. In the 10–30 nm size range, these particles exhibit superparamagnetic behavior: they respond rapidly to external magnetic fields but do not retain permanent magnetization, making them easy to manipulate and separate without harmful residual effects.

Dispersion in water creates a hydrophilic surface that enables direct compatibility with biological buffers and cells. Aqueous iron oxide nanoparticles are widely used to label and isolate specific cell populations, to functionalize protein detection assays, and to deliver therapeutics in research settings. Their magnetic properties allow rapid, non-invasive separation.

Key applications include:

• Magnetic cell sorting and enrichment from heterogeneous populations
• Nanoparticle-based protein and antibody purification
• Cell labeling for magnetic resonance imaging (MRI) and tracking
• Diagnostic immunoassays and biomarker detection

Usage & Handling Guidance

Store at 2–8°C. Before use, mix gently by inversion (do not vortex vigorously, which may damage the hydrophilic coating). The nanoparticles settle over time; resuspend as needed by gentle mixing or brief sonication.

• Magnetic separation: Add nanoparticles to your sample, incubate briefly to allow binding, then apply an external magnet to pull nanoparticles and bound material from solution (typically 30 seconds to 1 minute).
• pH and buffer compatibility: Verify compatibility with your specific assay buffer before starting; aqueous formulations are usually stable across a wide pH range (pH 4–9).
• Concentration: Request the lot-specific particle concentration and magnetization curve from the Certificate of Analysis for quantitative planning.

What You Get

• 2 mL of iron oxide nanoparticles (10–30 nm) in aqueous suspension
• Hydrophilic surface for immediate compatibility with biological buffers
• Strong superparamagnetic response for rapid separation
• For research use only (RUO)

Why Researchers Choose It

• Ready-to-use aqueous formulation—no solvent exchange required
• Superparamagnetic behavior allows rapid, non-damaging separation
• Hydrophilic surface compatible with wide range of biological assays
• Excellent platform for functional assays and biomarker detection

Frequently Asked Questions (FAQ)

• What iron oxide material is used?
  Typically magnetite (Fe₃O₄) or chemically similar iron oxides. Request the lot-specific composition in the Certificate of Analysis.
• How quickly will the nanoparticles separate from solution?
  With a standard handheld magnet, most aqueous iron oxide nanoparticles settle within 30 seconds to 1 minute. Stronger magnets (e.g., neodymium) accelerate separation.
• Are these particles pre-coated or functionalized?
  The particles are supplied with a hydrophilic surface for aqueous stability. For specific functional coatings (antibodies, aptamers, etc.), contact us for available options.
• Can I measure the concentration myself?
  Quantification can be done by inductively coupled plasma mass spectrometry (ICP-MS), atomic absorption spectroscopy (AAS), or via the saturation magnetization provided in the Certificate of Analysis.
• Do these particles work with my specific cell type?
  Compatibility depends on cell type, incubation conditions, and binding ligands. We recommend starting with a small pilot experiment before scaling up.

References

• Pucci C, Degl'Innocenti A, Belenli Gümüş M, Ciofani G. Superparamagnetic iron oxide nanoparticles for magnetic hyperthermia: recent advancements, molecular effects, and future directions in the omics era. Biomater Sci. 2022;10(9):2103-2121.
• Vangijzegem T, Stanicki D, Laurent S. Magnetic iron oxide nanoparticles for drug delivery: applications and characteristics. Expert Opin Drug Deliv. 2019;16(1):69-78.
• Dragar Č, Kralj S, Kocbek P. Bioevaluation methods for iron-oxide-based magnetic nanoparticles. Int J Pharm. 2021;597:120348.