Iron Oxide Nanoparticles in Chloroform, 5–30 nm – Catalog #B2026054

Iron Oxide Nanoparticles in Chloroform (Catalog #B2026054) consist of magnetic iron oxide particles (typically magnetite, Fe₃O₄) with a size range of 5–30 nm, dispersed in chloroform. The nanoparticles are strongly magnetic, allowing them to be rapidly separated and manipulated using external magnetic fields. Supplied as a 2 mL dispersion in organic solvent, these nanoparticles are used in magnetic separation, cell labeling, diagnostic assays, and materials research.

Scientific Overview

Iron oxide nanoparticles have emerged as powerful tools in biomedical research and diagnostics because of their strong, size-dependent magnetic properties and relative low toxicity. Magnetite (Fe₃O₄) and maghemite (γ-Fe₂O₃) are the most commonly used iron oxides. These nanoparticles exhibit superparamagnetic behavior at sizes below ~30 nm, allowing them to be magnetized and rapidly pulled from solution using an external magnet without retaining residual magnetization.

Dispersion in organic solvents such as chloroform maintains the hydrophobic surface of as-synthesized nanoparticles and prevents unwanted aggregation. Iron oxide nanoparticles have applications in magnetic cell separation, biomolecule labeling, magnetic hyperthermia, contrast enhancement in magnetic resonance imaging (MRI), and drug-delivery systems.

Key applications include:

• Magnetic separation of cells and biomolecules from complex mixtures
• Cell labeling for tracking and imaging
• Functional modifications and coating for bioconjugation
• Materials research into magnetic properties and nanoparticle synthesis

Usage & Handling Guidance

Handle chloroform dispersions with care in a fume hood; chloroform is volatile and has known health hazards. Store at room temperature in a sealed container, away from light and moisture. Before use, sonicate or vortex the dispersion to redisperse any settled particles.

• Magnetic separation: Apply an external magnet to pull nanoparticles from solution; allow a few seconds to 1 minute for collection depending on particle size and field strength.
• Solvent exchange: For aqueous applications, organic solvent can be exchanged for water or other solvents by centrifugation and redispersion, or by dialysis.
• Concentration: Request a Certificate of Analysis or Technical Data Sheet for the batch-specific concentration before use in quantitative assays.

What You Get

• 2 mL of iron oxide nanoparticles (5–30 nm) dispersed in chloroform
• Strong magnetic responsiveness for rapid separation
• Suitable for organic-solvent applications or for solvent exchange
• For research use only (RUO)

Why Researchers Choose It

• Stable, uniform particle size distribution in the superparamagnetic range
• Organic dispersion maintains hydrophobic surface for chemical modification
• Rapid, efficient magnetic separation without applied heating
• Versatile platform for bioconjugation and surface engineering

Frequently Asked Questions (FAQ)

• What is the actual composition of these nanoparticles?
  Iron oxide nanoparticles are typically magnetite (Fe₃O₄) or maghemite (γ-Fe₂O₃). Request the lot-specific composition in the Certificate of Analysis or Technical Data Sheet.
• Can I use these in aqueous applications?
  Yes, with solvent exchange. The nanoparticles can be transferred to water or other aqueous buffers by centrifugation and redispersion, or by dialysis against your target solvent.
• How strong is the magnetic response?
  Iron oxide nanoparticles in the 5–30 nm range are superparamagnetic and can be rapidly pulled from solution with a standard handheld magnet.
• Can these particles be coated or functionalized?
  Yes. The hydrophobic surface is well-suited to coating with silica, polymers, or conjugation with peptides or antibodies. Request information about available coating options.
• How do I measure the concentration?
  Request the lot-specific concentration and extinction coefficient or magnetization curve from the Certificate of Analysis, or contact us for titration guidance.

References

• Vangijzegem T, Stanicki D, Laurent S. Magnetic iron oxide nanoparticles for drug delivery: applications and characteristics. Expert Opin Drug Deliv. 2019;16(1):69-78.
• Nowak-Jary J, Machnicka B. Pharmacokinetics of magnetic iron oxide nanoparticles for medical applications. J Nanobiotechnology. 2022;20(1):305.
• Jacinto MJ, Silva VC, Valladão DMS, Souto RS. Biosynthesis of magnetic iron oxide nanoparticles: a review. Biotechnol Lett. 2021;43(1):1-12.