Hyaluronic Acid Sodium Salt from Rooster Comb – Research Use Only

Hyaluronic Acid Sodium Salt from Rooster Comb is a biotechnology-grade glycosaminoglycan supplied as a 50 mg powder for research applications involving biochemical assay development, matrix formulation, viscosity studies, and polysaccharide interaction workflows. As a sodium salt form of hyaluronan, this material is well suited for laboratories studying hydration behavior, rheological properties, macromolecular interactions, and glycosaminoglycan-based model systems. The powder format supports flexible preparation of working solutions in user-selected aqueous buffers for exploratory and method-development research.

Scientific Overview

Hyaluronic acid is a naturally occurring linear glycosaminoglycan composed of repeating disaccharide units of glucuronic acid and N-acetylglucosamine. In research settings, sodium hyaluronate is frequently used as a hydrating polymer, viscosity-modifying component, and model extracellular matrix polysaccharide for experiments involving diffusion, solution behavior, and biomolecular interaction studies.

Because the supplier lists molecular weight as N/A, this material is best incorporated into protocols where users can optimize concentration, viscosity, and performance empirically for their specific workflow. The powder format provides flexibility for preparing custom working solutions, screening concentrations, and adapting formulation conditions to suit analytical, biochemical, or proof-of-concept assay systems.

Applications

• Preparation of hyaluronan-containing research buffers and formulations
• Viscosity and rheology method development
• Polysaccharide-protein or polysaccharide-small molecule interaction studies
• Matrix and microenvironment modeling in biochemical workflows
• General glycosaminoglycan research and exploratory assay development

Usage & Handling Guidance

Store the powder at -20°C. For solution preparation, gradual wetting and slow mixing are recommended to support uniform hydration and minimize clumping. Working solutions may require additional mixing time depending on concentration and buffer composition. Researchers should establish appropriate preparation conditions based on the intended application.

• Recommended applications: Viscosity studies, glycosaminoglycan workflows, matrix preparation, and biochemical assay development
• Preparation: Reconstitute in suitable aqueous buffer and optimize concentration as needed
• Handling: Mix gradually to promote uniform dissolution and solution consistency
• Workflow note: Performance characteristics should be validated in the end-user protocol

What You Get

• 50 mg of Hyaluronic Acid Sodium Salt from Rooster Comb
• Biotechnology-grade powder suitable for laboratory research use
• Flexible format for custom buffer and formulation preparation
• Suitable for glycosaminoglycan and biochemical assay workflows
• For research use only (RUO)

Why Researchers Choose It

• Useful as a defined hyaluronan source for biochemical and formulation workflows
• Powder format supports flexible concentration and buffer optimization
• Applicable to viscosity, matrix, and interaction-based experimental systems
• Suitable for exploratory glycosaminoglycan and assay development work
• Convenient 50 mg amount for small-scale laboratory studies

Frequently Asked Questions (FAQ)

• What is this product used for in research?
  It is commonly used in biochemical and formulation workflows involving viscosity control, glycosaminoglycan studies, matrix preparation, and exploratory assay development.
• What form is this product supplied in?
  This product is supplied as a powder, allowing researchers to prepare custom working solutions in their preferred aqueous buffer systems.
• Is a molecular weight provided?
  No. The supplier lists molecular weight or concentration as N/A, so researchers should optimize concentration and performance based on their specific protocol requirements.
• How should this material be dissolved?
  Gradual wetting and slow mixing are recommended to support even hydration and reduce clumping during preparation.
• Can this be used in clinical or therapeutic applications?
  No. This product is supplied strictly for research use only and is not intended for diagnostic or therapeutic applications.

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References

• Swann DA. Studies on hyaluronic acid. I. The preparation and properties of rooster comb hyaluronic acid. Biochim Biophys Acta. 1968;156(1):17-30.
• Swann DA, Caulfield JB. Studies on hyaluronic acid. V. Relationship between the protein content and viscosity of rooster comb dermis hyaluronic acid. Connect Tissue Res. 1975;4(1):31-39.
• Laurent TC, Fraser JR. Hyaluronan. FASEB J. 1992;6(7):2397-2404.
• Kang DY, et al. Extraction of hyaluronic acid (HA) from rooster comb and characterization using flow field-flow fractionation (FlFFF) coupled with multiangle light scattering (MALS). J Sep Sci. 2010;33(22):3530-3536.
• Hafsa J, et al. Effect of ultrasonic degradation of hyaluronic acid extracted from rooster comb on antioxidant and antiglycation activities. Pharm Biol. 2017;55(1):156-163.
• Giji S, Arumugam M. Isolation and characterization of hyaluronic acid from marine organisms. Adv Food Nutr Res. 2014;72:61-77.
• Jacobson B. Hyaluronic acid synthesis in rooster comb. Effect of testosterone on nucleotide sugar metabolism. Connect Tissue Res. 1978;5(4):217-223.
• Reed CE, Li X, Reed WF. The effects of pH on hyaluronate as observed by light scattering. Biopolymers. 1989;28(11):1981-2000.
• Manfrão-Netto JHC, et al. Genetic strategies for improving hyaluronic acid production in recombinant bacterial culture. J Appl Microbiol. 2022;132(2):822-840.