Human Interferon‑alpha2b (Highly Pure) – Research Use Only

Human Interferon‑alpha2b (IFN‑α2b) is a highly purified recombinant cytokine expressed in HEK293 cells and supplied as a lyophilized powder. IFN‑α2b is a member of the type I interferon family and functions as a key regulator of innate immune signaling. It activates interferon‑stimulated gene pathways through the JAK‑STAT signaling cascade and is widely used in research involving antiviral responses, immune modulation, cytokine biology, and cell‑signaling studies. This preparation is biotechnology‑grade and suitable for in vitro biochemical and cell‑based research applications.

Scientific Overview

Interferon‑alpha2b is a type I interferon involved in antiviral defense, immune regulation, and modulation of cellular signaling pathways. It binds to the IFNAR1/IFNAR2 receptor complex, activating JAK1 and TYK2 kinases and inducing transcription of interferon‑stimulated genes (ISGs). These pathways regulate innate immune responses, cellular stress responses, and cytokine networks. Recombinant IFN‑α2b is widely used in mechanistic studies of interferon signaling, host–pathogen interactions, and immune pathway activation.

Common Research Applications

• Innate immune signaling and interferon pathway studies
• JAK‑STAT activation assays
• Host–pathogen interaction research
• Cell‑based cytokine response modeling
• Gene expression studies involving interferon‑stimulated genes (ISGs)
• Biochemical and structural studies of type I interferons

Usage & Handling Guidance

Reconstitute in sterile buffer to the desired concentration. Avoid repeated freeze–thaw cycles by aliquoting upon reconstitution. Store lyophilized material at -20 °C and reconstituted protein at -80 °C. Use low‑binding tubes and maintain sterile technique for cell‑based assays.

• Storage (lyophilized): -20 °C
• Storage (reconstituted): -80 °C
• Handling: Aliquot to avoid freeze–thaw cycles
• Compatibility: Cell‑based assays, biochemical assays, signaling studies

What You Get

• 10 µg highly purified recombinant Human Interferon‑alpha2b
• Lyophilized powder for flexible reconstitution
• Batch‑specific documentation available upon request
• For research use only (RUO)

Frequently Asked Questions

• What is the source of this recombinant IFN‑α2b?
  This protein is expressed in HEK293 cells and purified to biotechnology‑grade specifications.
• Is this product suitable for cell‑based assays?
  Yes. IFN‑α2b is commonly used in cell‑based signaling and cytokine response studies.
• Does this product have antiviral activity?
  IFN‑α2b participates in antiviral signaling pathways, but this preparation is intended strictly for in vitro research use and is not validated for clinical or therapeutic applications.
• How should the protein be reconstituted?
  Reconstitute in sterile buffer (e.g., PBS or cell‑culture medium) to the desired concentration and aliquot immediately.
• Can I request a COA or TDS?
  Yes. COA/TDS availability is batch dependent. Contact us with the catalog and lot number.
• Is this product suitable for diagnostic or therapeutic use?
  No. This reagent is intended exclusively for in vitro research use only (RUO).

---

References

• Ishikawa T, Higuchi K, Kubota T, Seki K, Honma T, Yoshida T, Kamimura T. Combination PEG‑IFN‑α2b/ribavirin therapy following treatment of hepatitis C virus‑associated hepatocellular carcinoma is capable of improving hepatic functional reserve and survival. Hepatogastroenterology. 2012 Mar–Apr;59(114):529‑532.