Human IgM Conjugated to Agarose Beads, High-Concentration, 2 mL – Catalog #B2025629

Human IgM Conjugated to Agarose Beads – High-Concentration (Catalog #B2025629) is a high-density immobilized form of human Immunoglobulin M providing enhanced capacity for antigen capture and immune complex isolation. Supplied as 2 mL of premium-grade suspension, this conjugate is optimized for applications requiring maximum binding capacity and multi-valent interactions. The pentameric structure of IgM (approximately 900 kDa) with five Fab domains ensures simultaneous engagement of multiple epitopes, improving both sensitivity and specificity in immunological research.

Scientific Overview

Immunoglobulin M (IgM) is the largest antibody isotype, composed of five identical monoclonal units (each ~50 kDa Fab domain) held together by a J chain, yielding a total molecular weight of approximately 900 kDa. Its pentameric architecture gives IgM exceptional avidity and the ability to cross-link multiple antigens. IgM is the first antibody produced in primary immune responses and is a potent activator of the classical complement pathway, making it a valuable research reagent for detecting and capturing diverse antigens and immune complexes.

The high-concentration formulation of this product delivers increased IgM density per unit of agarose beads, enabling enhanced binding capacity and faster kinetics for applications demanding high-throughput processing or large-volume sample handling.

Key applications include:

• High-capacity affinity chromatography for antigen purification from crude extracts
• Immunoprecipitation of abundant antigens and immune complexes
• Complement-mediated separation and concentration of target antigens
• Flow cytometry sample preparation and cell enrichment

Usage & Handling Guidance

Store at 2–8°C. Allow beads to warm to room temperature before use. Mix gently to resuspend beads; do not vortex vigorously. Avoid repeated freeze–thaw cycles, which may reduce capacity.

• High-capacity protocols: The increased IgM density supports larger sample volumes and higher antigen concentrations; pre-titrate with your specific antigen to determine optimal bead:antigen ratio.
• Binding conditions: Incubate at 4°C for maximum specificity or room temperature for increased kinetics; higher IgM density reduces incubation time.
• Elution: Use standard protocols (0.1 M glycine pH 2.5, 3 M NaCl, or other low-pH conditions) followed by immediate pH neutralization.
• Regeneration: Beads can be regenerated and reused multiple times; capacity is retained over several cycles with proper care.

What You Get

• 2 mL of high-concentration human IgM conjugated to agarose beads, supplied as a suspension
• Premium-grade affinity support with enhanced binding capacity
• Optimized for high-throughput and large-scale antigen capture protocols
• For research use only (RUO)

Why Researchers Choose It

• High IgM density for maximum binding capacity and faster kinetics
• Pentameric structure ensures high avidity and multi-point antigen engagement
• Efficient complement activation useful in specialized immunological assays
• Suitable for both batch and column-based chromatography workflows

Frequently Asked Questions (FAQ)

• Why choose high-concentration over standard IgM agarose beads?
  High-concentration formulations enable faster reactions, require fewer beads per reaction, and are ideal for high-throughput screening or processing large sample volumes.
• How does the pentameric structure improve antigen capture?
  Each IgM molecule has five Fab domains, allowing simultaneous binding to multiple epitopes on a single antigen or cross-linking of multiple antigens, increasing both avidity and sensitivity.
• What is the expected binding capacity?
  Capacity depends on antigen size, concentration, and binding conditions; request a COA/TDS or contact us for lot-specific binding capacity data.
• Can these beads be used with automated systems?
  Yes. The suspension format is compatible with standard pipetting and many automated liquid handlers; confirm specifications with us if using specialized equipment.
• How many times can I regenerate these beads?
  Beads typically support multiple reuse cycles (5–10 or more) with proper care and appropriate elution buffers; capacity may diminish slightly with each cycle.

References

• Yang S, Zhao LC, Guo FZ, Xu HS, Huang MM, Fei H. Evolution of immunoglobulin M (IgM) structure and function in vertebrates: A comprehensive review. Fish Shellfish Immunol. 2025;166:110635.
• Buchner J, Sitia R, Svilenov HL. Understanding IgM Structure and Biology to Engineer New Antibody Therapeutics. BioDrugs. 2025;39(3):347-357.
• Salehi N, Peng CA. Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography. Biotechnol Prog. 2016;32(4):949-58.