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High HAMA Serum Serum & Plasma Molecular Depot
High HAMA Serum Serum & Plasma Molecular Depot

High HAMA Serum

$595.00

    Catalog #: S2010006


    High HAMA Serum is a 1 mL biotechnology-grade human serum matrix containing elevated Human Anti-Mouse Antibodies (HAMA) used for immunoassay interference testing, assay validation, and analytical robustness studies. This HAMA-positive serum is ideal for evaluating heterophilic antibody interference, false-positive signal risk, and blocking strategy effectiveness in ELISA, CLIA, and antibody-based assay workflows. The aliquoted serum format supports controlled matrix spiking and reproducible assay development experiments.

    Products are for in vitro research use only (RUO).

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High HAMA Serum – Research Use Only

High HAMA Serum is a biotechnology-grade human serum interference testing reagent supplied as a 1 mL aliquot for research applications involving immunoassay validation, heterophilic antibody interference studies, and analytical robustness testing. This serum contains elevated levels of Human Anti-Mouse Antibodies (HAMA), which are known to interfere with immunoassays that utilize murine monoclonal antibodies. It is especially useful in workflows related to ELISA, CLIA, biomarker quantitation, diagnostic assay development, and assay troubleshooting.

Catalog # S2010006
Lot # Batch dependent
Expiration Date Batch dependent
Volume 1 mL
Appearance Yellow orange
Matrix Human serum
Storage -20°C or lower. Avoid repeated freeze-thaw cycles. Aliquot as needed.
Keywords HAMA sample, Human Anti-Mouse Antibodies serum, High HAMA plasma

Scientific Overview

Human Anti-Mouse Antibodies (HAMA) are a well-documented source of heterophilic antibody interference in immunoassays that rely on murine monoclonal antibodies. These antibodies can bind nonspecifically to assay components, leading to false-positive signals, altered binding kinetics, and inaccurate quantitation.

In laboratory and assay development settings, high HAMA serum is commonly used to evaluate assay susceptibility to interference, blocking strategy effectiveness, and analytical reliability. It is particularly relevant in workflows involving two-site immunoassays, sandwich ELISAs, chemiluminescent assays, and biomarker detection systems.

Applications

  • Immunoassay interference testing and validation
  • Heterophilic antibody interference evaluation
  • ELISA and CLIA assay development and troubleshooting
  • Matrix spiking and assay robustness studies
  • Blocking reagent validation and optimization workflows

Usage & Handling Guidance

Store at -20°C or lower as specified. To preserve sample integrity, avoid repeated freeze-thaw cycles and aliquot upon first use if necessary. This serum may be used directly or diluted depending on the intended assay validation or interference testing protocol.

  • Preparation: Use directly or dilute into assay matrix as required
  • Handling: Minimize freeze-thaw cycles to maintain consistency
  • Optimization: Adjust spike levels based on assay sensitivity and interference design
  • Workflow note: Validate assay performance under HAMA-challenged conditions

What You Get

  • 1 mL High HAMA human serum sample
  • Elevated HAMA levels for interference testing workflows
  • Biotechnology-grade material for assay validation
  • Suitable for ELISA, CLIA, and immunoassay systems
  • For research use only (RUO)

Why Researchers Choose It

  • Designed specifically for heterophilic antibody interference studies
  • Supports assay validation and robustness testing
  • Relevant to real-world immunoassay performance challenges
  • Useful for troubleshooting false positives and signal distortion
  • Convenient aliquot format for controlled experimental use

Frequently Asked Questions (FAQ)

  • What is High HAMA Serum used for?
    It is used for immunoassay interference testing, particularly to evaluate the impact of human anti-mouse antibodies on assay performance.
  • Why are HAMA antibodies important in assay development?
    HAMA can bind nonspecifically to assay antibodies, causing false positives or altered signal behavior in immunoassays.
  • What types of assays are affected by HAMA?
    HAMA interference is especially relevant in ELISA, CLIA, sandwich immunoassays, and other antibody-based detection systems.
  • How should this product be handled?
    Store frozen and avoid repeated freeze-thaw cycles. Aliquot if needed to maintain consistency across experiments.
  • Is this product intended for diagnostic or clinical use?
    No. This product is for Research Use Only (RUO) and is not intended for diagnostic or therapeutic applications.
This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

  • Reinsberg J. Different efficacy of various blocking reagents to eliminate interferences by human antimouse antibodies with a two-site immunoassay. Clin Biochem. 1996 Apr;29(2):145-8.
  • Bolstad N, Warren DJ, Nustad K. Heterophilic antibody interference in immunometric assays. Best Pract Res Clin Endocrinol Metab. 2013 Oct;27(5):647-61.
  • Lippi G, Aloe R, Meschi T, Borghi L, Cervellin G. Interference from heterophilic antibodies in troponin testing. Clin Chim Acta. 2013 Nov 15;426:79-84.
  • Kawasaki H et al. Minos-insertion mutant of the Drosophila GBA gene homologue showed abnormal phenotypes. Gene. 2017 May 30;614:49-55.
  • Hull B. Aberrantly elevated TSH level due to HAMA interference with thyrotropin assay. J S C Med Assoc. 2012 Feb;108(1):12-3.
  • Degn SE et al. Assay interference caused by antibodies reacting with rat kappa light-chain in human sera. J Immunol Methods. 2011 Sep 30;372(1-2):204-8.

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

    Purchase Orders
    Confirmed orders are final and may not be canceled or refunded unless authorized in writing.

    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

    Safety Guidelines
    Always consult the product’s SDS. Products must not be ingested, inhaled, or come into contact with skin or eyes unless specified.

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    Products must not be used in violation of any law—local, state, federal, or international.

    Accuracy of Orders
    Customers are responsible for verifying all order details before submission.

    Changes or Cancellations
    All changes must be in writing and approved. Cancellations may result in fees for materials, labor, or third-party costs.

    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

    Delivery & Claims
    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

    Extended Payment Terms
    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
    Ownership remains with Blue Tiger Scientific or Molecular Depot LLC until payment is complete. Goods must be stored separately and labeled until paid in full.

    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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