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HEPES Buffer (0.5 M, pH 7.0) Chemicals Molecular Depot
HEPES Buffer (0.5 M, pH 7.0) Chemicals Molecular Depot

HEPES Buffer (0.5 M, pH 7.0)

$907.00

    Catalog Number: B2018133 (100 mL)

    HEPES buffer is a commonly used biological buffer in cell culture and molecular biology experiments. It stands for 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid. The buffer is prepared at a concentration of 0.5 moles per liter and adjusted to a pH of 7.0. This buffer is often used to maintain a stable pH in cell culture media and biochemical reactions. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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HEPES Buffer (0.5 M, pH 7.0)

Catalog number: B2018133
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: 2-8℃
Keywords: HEPES
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References

  • Obeid NJ, Hadwan MH. A quantitative method for the assessment of homocysteine thiolactonase enzyme activity Anal Biochem. 2021 Oct 15;631:114365.
  • Areti S, Bandaru S, Teotia R, Rao CP. Water-Soluble 8-Hydroxyquinoline Conjugate of Amino-Glucose As Receptor for La(3+) in HEPES Buffer, on Whatman Cellulose Paper and in Living Cells Anal Chem. 2015 Dec 15;87(24):12348-54.
  • Törnquist K, Päällysaho J, Heinonen E, Kaila K. Influence of Hepes- and CO2/HCO(3-)-buffer on Ca2+ transients induced by TRH and elevated K+ in rat pituitary GH4C1 cells Mol Cell Endocrinol. 1995 Jul;112(1):77-82.
  • Grady JK, Chasteen ND, Harris DC. Radicals from “Good’s” buffers Anal Biochem. 1988 Aug 15;173(1):111-5.
  • MacGregor DG, Chesler M, Rice ME. HEPES prevents edema in rat brain slices Neurosci Lett. 2001 May 11;303(3):141-4.
  • Lee H, Kim HJ. Fluorescein aldehyde with disulfide functionality as a fluorescence turn-on probe for cysteine and homocysteine in HEPES buffer Org Biomol Chem. 2013 Aug 14;11(30):5012-6.
  • Serizawa T, Hirai Y, Aizawa M. Detection of enzyme activities based on the synthesis of gold nanoparticles in HEPES buffer Mol Biosyst. 2010 Sep;6(9):1565-8.
  • Ganz MB, Rasmussen J, Bollag WB, Rasmussen H. Effect of buffer systems and pHi on the measurement of [Ca2+]i with fura 2 FASEB J. 1990 Apr 1;4(6):1638-44.
  • Sun A, Mirzayans PM, Piggott AM, Stanton JL, Sunna A. Adapted method for rapid detection and quantification of pathogen Campylobacter jejuni from environmental water samples FEMS Microbiol Ecol. 2023 Jun 16;99(7):fiad058.

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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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