Hemopexin from Rat Serum – Research Use Only

Hemopexin from Rat Serum is a high-purity glycoprotein (≈60 kDa) supplied as a 50 µg solution (Catalog #B2015325). Hemopexin is the primary heme-binding protein in plasma. It binds free heme with extremely high affinity, prevents heme-mediated oxidative damage, and delivers heme to hepatocytes via receptor-mediated endocytosis. This rat-derived hemopexin is an excellent research tool for studying heme metabolism, oxidative stress, inflammation, and acute-phase responses in rodent models.

Scientific Overview

Hemopexin is an acute-phase glycoprotein that binds free heme with the highest known affinity of any protein. By sequestering heme, it prevents heme-catalyzed oxidative damage and lipid peroxidation while facilitating safe heme recycling in the liver. In rat models, hemopexin levels rise during inflammation, infection, and tissue injury, making it a useful marker of the acute-phase response. Purified rat hemopexin is widely used to study heme transport, protection against heme toxicity, and interactions with heme oxygenase pathways.

This Hemopexin from Rat Serum is suitable for:

• Heme-binding and transport studies
• Investigation of oxidative stress and heme-mediated damage
• Acute-phase response and inflammation research in rat models
• Development of heme-related biomarker assays
• Studies on hemopexin–receptor interactions and heme recycling

Usage & Handling Guidance

Store at -20 °C. Thaw on ice and keep cold during use. Avoid repeated freeze–thaw cycles by preparing single-use aliquots. Dilute in appropriate assay buffer (e.g., PBS with 0.1% BSA) immediately before use. The protein remains stable when handled under standard laboratory conditions.

• Recommended applications: Heme-binding assays, oxidative stress studies, and acute-phase response research
• Working concentration: Optimize empirically (commonly 1–50 µg/mL depending on assay)
• Stability: Stable when stored frozen; minimize freeze–thaw cycles
• Handling: Use protease-free conditions; keep on ice during dilution

What You Get

• 50 µg Hemopexin from Rat Serum as a solution
• High-purity rat-derived hemopexin suitable for biochemical and immunological studies
• Native-like glycoprotein for accurate heme-binding and functional assays
• Convenient quantity for multiple experiments and method optimization
• For research use only (RUO)

Why Researchers Choose It

• High-purity rat hemopexin for species-relevant studies
• Excellent tool for heme-binding, oxidative stress, and acute-phase response research
• Useful as a control or standard in rodent inflammation and injury models
• Stable solution format with reliable lot-to-lot consistency
• Well-characterized protein with extensive literature support in heme metabolism

Frequently Asked Questions (FAQ)

• What is the primary function of hemopexin?
  Hemopexin binds free heme with very high affinity, preventing oxidative damage and facilitating safe heme recycling in the liver.
• Why use rat hemopexin?
  It is ideal for experiments in rat models of inflammation, oxidative stress, and acute-phase responses.
• How should I store the product?
  Store at -20 °C and avoid repeated freeze–thaw cycles by making aliquots.
• Can this be used in functional heme-binding assays?
  Yes. It retains native heme-binding capacity and is suitable for spectroscopic or binding studies.
• Is this suitable for cell culture?
  It can be used in cell-based assays when properly diluted in serum-free or low-serum media.

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References

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• Madore N, Camborieux L, Bertrand N, Swerts JP. Regulation of hemopexin synthesis in degenerating and regenerating rat sciatic nerve. J Neurochem. 1999 Feb;72(2):708-15.
• Noiva R, Pete MJ, Babin DR. Bovine serum hemopexin: properties of the protein from a single animal. Comp Biochem Physiol B. 1987;88(1):341-7.
• Dong B, Bai Y, Jia Q, Su L, Zhang X, Wang S, Fang Z. Screening Serum Biomarkers for Rats Preconditioned with Hyperbaric Oxygen: Potential of Predicting Prognosis for Stroke. J Integr Neurosci. 2023 Jul 20;22(4):103.
• Clapper ML, Clark ME, Klein NW, Kurtz PJ, Carlton BD, Chhabra RS. Cardiovascular defects in rat embryos cultured on serum from rats chronically exposed to phenytoin. Teratog Carcinog Mutagen. 1986;6(2):151-61.
• Jung JY, Kwak YH, Kim KS, Kwon WY, Suh GJ. Change of hemopexin level is associated with the severity of sepsis in endotoxemic rat model and the outcome of septic patients. J Crit Care. 2015 Jun;30(3):525-30.
• Ingoglia G, Sag CM, Rex N, De Franceschi L, Vinchi F, Cimino J, Petrillo S, Wagner S, Kreitmeier K, Silengo L, Altruda F, Maier LS, Hirsch E, Ghigo A, Tolosano E. Hemopexin counteracts systolic dysfunction induced by heme-driven oxidative stress. Free Radic Biol Med. 2017 Jul;108:452-464.
• Ikawa M, Shozen Y. Quantification of acute phase proteins in rat serum and in the supernatants of a cultured rat hepatoma cell line and cultured primary hepatocytes by an enzyme-linked immunosorbent assay. J Immunol Methods. 1990 Nov 6;134(1):101-6.
• Dong B, Zhang Z, Xie K, Yang Y, Shi Y, Wang C, Yu Y. Hemopexin promotes angiogenesis via up-regulating HO-1 in rats after cerebral ischemia-reperfusion injury. BMC Anesthesiol. 2018 Jan 3;18(1):2.
• Vincent SH, Holeman B, Cully BC, Muller-Eberhard U. Porphyrin-induced photodynamic cross-linking of hepatic heme-binding proteins. Life Sci. 1986 Jan 27;38(4):365-72.