Green Fluorescent Protein (GFP) – Research Use Only

Green Fluorescent Protein (GFP), originally isolated from the jellyfish Aequorea victoria, is a 27–28 kDa intrinsically fluorescent protein that emits bright green light upon excitation with blue or UV light. Supplied as a 300 µg solution (Catalog #B2012447), this biotechnology-grade recombinant GFP serves as a cornerstone molecular tool for live-cell imaging, protein localization, expression monitoring, fusion protein tracking, and as a standard in fluorescence-based assays.

Scientific Overview

Green Fluorescent Protein (GFP) is a spontaneously fluorescent protein that requires no cofactors or substrates for fluorescence, making it one of the most versatile tools in modern cell and molecular biology. Its β-barrel structure encapsulates a chromophore formed by autocatalytic cyclization of three internal amino acids, enabling bright green emission upon excitation. GFP and its engineered variants (e.g., EGFP, superfolder GFP) are widely used for real-time visualization of protein dynamics, subcellular localization, promoter activity, and protein–protein interactions in living cells and organisms.

This recombinant Green Fluorescent Protein is suitable for:

• Live-cell imaging and time-lapse microscopy
• Protein tagging and subcellular localization studies
• Monitoring gene expression and transfection efficiency
• Flow cytometry and fluorescence-activated cell sorting (FACS)
• Development and calibration of fluorescence-based assays

Usage & Handling Guidance

Store at –20 °C. Thaw on ice and keep the solution cold during use. Avoid repeated freeze–thaw cycles by preparing single-use aliquots. The protein is supplied in a stabilized solution and can be used directly or diluted into appropriate buffers for experimental applications.

• Recommended applications: Live-cell imaging, fusion protein tracking, and fluorescence assays
• Working concentration: Typically 1–10 µg/mL for imaging or 0.1–1 mg/mL for biochemical standards (optimize empirically)
• Stability: Stable when stored frozen; protect from prolonged light exposure during experiments
• Handling: Use sterile techniques; keep on ice when working with the solution

What You Get

• 300 µg recombinant Green Fluorescent Protein (GFP) as a ready-to-use solution
• ~28 kDa intrinsically fluorescent protein with bright green emission
• High-purity biotechnology-grade material suitable for sensitive imaging and biochemical applications
• Convenient format for immediate use in cell biology and protein studies
• For research use only (RUO)

Why Researchers Choose It

• Classic, bright intrinsically fluorescent protein – no external cofactors required
• Versatile molecular tag for live-cell imaging, protein localization, and expression monitoring
• High-purity biotechnology-grade formulation ensures consistent fluorescence and performance
• Ready-to-use solution format simplifies experimental workflows
• Well-characterized tool with extensive literature support across cell and molecular biology

Frequently Asked Questions (FAQ)

• What is the excitation and emission wavelength of this GFP?
  Classic GFP has major excitation at ~395 nm and emission at ~509 nm (green).
• Is this wild-type GFP or an engineered variant?
  This is recombinant GFP based on the original sequence from Aequorea victoria.
• Can it be used for fusion proteins?
  Yes. GFP is commonly fused to proteins of interest for localization and tracking studies.
• How should I store the solution?
  Store at –20 °C. Aliquot upon first thaw to avoid repeated freeze–thaw cycles.
• Is this suitable for flow cytometry?
  Yes. GFP is widely used in flow cytometry and FACS for detecting transfected or transduced cells.

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References

• Remington SJ. Green fluorescent protein: a perspective. Protein Sci. 2011 Sep;20(9):1509-19.
• Shorter SA, Pettit MW, Dyer PDR, Coakley Youngs E, Gorringe-Pattrick MAM, El-Daher S, Richardson S. Green fluorescent protein (GFP): is seeing believing and is that enough? J Drug Target. 2017 Nov-Dec;25(9-10):809-817.
• Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Engineering and characterization of a superfolder green fluorescent protein. Nat Biotechnol. 2006 Jan;24(1):79-88.
• Kong J, Wang Y, Qi W, Huang M, Su R, He Z. Green fluorescent protein inspired fluorophores. Adv Colloid Interface Sci. 2020 Nov;285:102286.
• Pedelacq JD, Cabantous S. Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology. Int J Mol Sci. 2019 Jul 15;20(14):3479.
• Shimomura O. Discovery of green fluorescent protein (GFP) (Nobel Lecture). Angew Chem Int Ed Engl. 2009;48(31):5590-602.
• Wei T, Dai H. Quantification of GFP signals by fluorescent microscopy and flow cytometry. Methods Mol Biol. 2014;1163:23-31.
• Seibel NM, Eljouni J, Nalaskowski MM, Hampe W. Nuclear localization of enhanced green fluorescent protein homomultimers. Anal Biochem. 2007 Sep 1;368(1):95-9.
• Cabantous S, Terwilliger TC, Waldo GS. Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nat Biotechnol. 2005 Jan;23(1):102-7.
• Lipták N, Bősze Z, Hiripi L. GFP transgenic animals in biomedical research: a review of potential disadvantages. Physiol Res. 2019 Aug 29;68(4):525-530.