β-Galactosidase Conjugated to Streptavidin – Catalog Number: B2012441 (2 mL)

β-Galactosidase Conjugated to Streptavidin is supplied as a biotechnology-grade solution in a defined 2 mL fill (Catalog B2012441) with storage specified at 2–8 °C. The supplier lists this item as a molecular tool for various biochemical applications and identifies it explicitly as a Streptavidin–β-Galactosidase conjugate. In research workflows, this pairing leverages the strong biotin–streptavidin interaction to connect biotinylated capture elements with the well-characterized catalytic activity of β-galactosidase, enabling signal generation on chromogenic or fluorogenic reporter substrates. The clear documentation fields—amount, supplied-as, storage, keyword identity, and grade—make SOP inclusion and inventory control straightforward for multi-user labs.

Because the listing does not prescribe a working concentration or activity, establishing conditions empirically is good practice. Start with small pilot titrations designed around your assay matrix and detection chemistry. Record buffer composition, pH, incubation times, and time-at-temperature, and retain lot identifiers to ensure reproducibility across runs and analysts. The ready-to-use solution format minimizes variability associated with reconstitution and supports rapid buffer-compatibility checks when optimizing signal windows, background behavior, and washing protocols. When used with biotinylated targets, streptavidin provides high-affinity anchoring while the β-gal moiety converts appropriate substrates to measurable products, which can be read on standard plate readers, imaging systems, or membranes depending on assay design.

Typical use cases in method development include building qualitative capture/detection chains for biotinylated analytes, configuring benchtop training exercises that require a robust enzyme label, or creating platform checks where predictable enzyme behavior simplifies troubleshooting. The biotechnology-grade note—solutions prepared using Type I ultrapure water and fine filtration—aligns with expectations for low background during optimization. Within research-only boundaries, this conjugate provides a dependable, clearly specified component for laboratories standardizing biotin-enabled detection schemes that utilize β-galactosidase catalysis.

Why researchers choose this product:

• Defined 2 mL fill in a ready-to-use solution format for rapid integration
• High-affinity streptavidin interface supports biotin-enabled capture and detection
• Specified storage at 2–8 °C fits routine refrigerator handling
• Concise documentation fields (amount, supplied-as, storage, keywords) streamline SOP/LIMS mapping
• Biotechnology-grade note (Type I water; 0.22 µm filtration for solutions) supports low background

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

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