EDTA Dipotassium Salt K2 EDTA (10% Solution) – Research Use Only

EDTA Dipotassium Salt K2 EDTA (10% Solution) is a biotechnology-grade chelating agent supplied as a 1 mL ready-to-use 10% (w/v) aqueous solution. K2 EDTA effectively binds divalent metal ions (especially Ca²⁺ and Mg²⁺), making it an essential reagent for inhibiting metalloenzymes, preventing coagulation, stabilizing nucleic acids, and performing interference testing in biochemical and analytical workflows.

Scientific Overview

Dipotassium EDTA (K2 EDTA) is a powerful chelating agent that forms stable complexes with divalent and trivalent metal ions, particularly calcium and magnesium. By sequestering these ions, it prevents blood coagulation, inhibits metal-dependent enzymes, and stabilizes many labile biomolecules. The 10% (w/v) solution is widely used in hematology, molecular biology, clinical chemistry, and analytical method development, especially for interference testing and sample preservation.

This K2 EDTA (10% Solution) is suitable for:

• Anticoagulation and blood sample preservation
• Inhibition of metalloenzymes and nucleases
• Interference testing in LC-MS/MS, immunoassays, and other analytical methods
• Stabilization of nucleic acids and proteins during extraction or storage
• Preparation of calibration standards and quality control matrices

Usage & Handling Guidance

Store the 10% solution at -20 °C. Thaw on ice or at 2–8 °C and mix gently before use. Aliquot upon first thaw to minimize repeated freeze–thaw cycles. Use at appropriate working concentrations (typically 1–10 mM final concentration depending on the application).

• Recommended applications: Sample anticoagulation, enzyme inhibition, interference testing, and biomolecule stabilization
• Working concentration: Dilute to achieve 1–10 mM final EDTA concentration in the sample or buffer (optimize for specific assay)
• Compatibility: Compatible with most aqueous biochemical and analytical workflows
• Handling: Use in a clean environment; the solution is pre-filtered (0.22 µm) for reduced contamination risk

What You Get

• 1 mL of 10% (w/v) K2 EDTA solution
• High-purity chelating agent prepared with Type I ultrapure water and 0.22 µm filtration
• Ready-to-use format for anticoagulation, enzyme inhibition, and interference studies
• Batch-specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Effective chelation of Ca²⁺ and Mg²⁺ for anticoagulation and enzyme inhibition
• Ideal for interference testing and surrogate matrix preparation in LC-MS/MS and immunoassays
• High-purity 10% solution ensures consistency and low background
• Useful in hematology, molecular biology, and clinical chemistry research
• Convenient small-volume format for method development and validation

Frequently Asked Questions (FAQ)

• What is the concentration and form of this product?
  It is a 10% (w/v) solution of dipotassium EDTA (K2 EDTA) supplied as 1 mL liquid.
• Why use K2 EDTA instead of other forms?
  K2 EDTA is preferred in many analytical applications because it provides excellent analyte stability and is the standard anticoagulant for hematology and plasma-based assays.
• What is the recommended working concentration?
  Typical final concentrations in samples or buffers range from 1–10 mM. Optimize for your specific assay or interference testing protocol.
• Is this solution sterile?
  Yes. It is prepared with Type I ultrapure water and filtered through 0.22 µm membranes.
• Can this be used for blood collection?
  This concentrated solution is intended for research use (e.g., interference testing or matrix preparation), not for direct blood collection tubes.

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References

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• Krawczyk MK, Lis T. New coordination modes in potassium edta salts: K2[H2edta]2H2O, K3[Hedta]2H2O and K4[edta]·3.92H2O. Acta Crystallogr C. 2011 Jul;67(Pt 7):m266-74.
• Jiang L, Ding X, Wang W, Yang X, Li T, Lei P. Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma. Biomolecules. 2022 Aug 28;12(9):1194.