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E. coli Methionine Aminopeptidase (MAP) Proteins Molecular Depot
E. coli Methionine Aminopeptidase (MAP) Proteins Molecular Depot
E. coli Methionine Aminopeptidase (MAP) Proteins Molecular Depot
E. coli Methionine Aminopeptidase (MAP) Proteins Molecular Depot

E. coli Methionine Aminopeptidase (MAP)

$907.00

    Catalog Number: B2015148 (10 ug)

    E. coli Methionine Aminopeptidase (MAP) is a high quality recombinant E. coli Methionine Aminopeptidase (MAP). This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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E. coli Methionine Aminopeptidase (MAP)

Catalog number: B2015148
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 10 ug
Molecular Weight or Concentration: 31.5 kDa (284aa)
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -20 °C
Keywords: MetAP, Peptidase M
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References

  • Wingfield PT. N-Terminal Methionine Processing Curr Protoc Protein Sci. 2017 Apr 3;88:6.14.1-6.14.3.
  • Ben-Bassat A, Bauer K, Chang SY, Myambo K, Boosman A, Chang S. Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure J Bacteriol. 1987 Feb;169(2):751-7.
  • Arif A, Mohammed K, Nadeem MS. Biochemical and in silico evaluation of recombinant E. coli aminopeptidase and in vitro processed human interferon α-2b Turk J Biol. 2018 Jun 13;42(3):240-249.
  • Roderick SL, Matthews BW. Structure of the cobalt-dependent methionine aminopeptidase from Escherichia coli: a new type of proteolytic enzyme Biochemistry. 1993 Apr 20;32(15):3907-12.
  • Chang SY, McGary EC, Chang S. Methionine aminopeptidase gene of Escherichia coli is essential for cell growth J Bacteriol. 1989 Jul;171(7):4071-2.
  • Tsunasawa S, Izu Y, Miyagi M, Kato I. Methionine aminopeptidase from the hyperthermophilic Archaeon Pyrococcus furiosus: molecular cloning and overexpression in Escherichia coli of the gene, and characteristics of the enzyme J Biochem. 1997 Oct;122(4):843-50.
  • You C, Lu H, Sekowska A, Fang G, Wang Y, Gilles AM, Danchin A. The two authentic methionine aminopeptidase genes are differentially expressed in Bacillus subtilis BMC Microbiol. 2005 Oct 5;5:57.
  • Datta B. MAPs and POEP of the roads from prokaryotic to eukaryotic kingdoms Biochimie. 2000 Feb;82(2):95-107.
  • Shapiro AB, Gao N, Thresher J, Walkup GK, Whiteaker J. A high-throughput absorbance-based assay for methionine produced by methionine aminopeptidase using S-adenosyl-L-methionine synthetase J Biomol Screen. 2011 Jun;16(5):494-505.

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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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