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Concanavalin A Lectin (Con A) – Texas Red Proteins Molecular Depot
Anti-GFP Magnetic Beads Beads & Particles Molecular Depot
Concanavalin A Lectin (Con A) – Texas Red Proteins Molecular Depot
Anti-GFP Magnetic Beads Beads & Particles Molecular Depot

Concanavalin A Lectin (Con A) – Texas Red

$1,175.00

    Catalog Number: B2025513 (2 mg)

    Concanavalin A – Texas Red (Catalog #B2025513) is the jack bean (Canavalia ensiformis) lectin conjugated to Texas Red, a bright red fluorophore (Ex/Em ~589/615 nm). Con A binds α-D-mannosyl and α-D-glucosyl residues, including the trimannoside core of N-linked glycans, making this conjugate a reliable probe for visualizing cell-surface glycoproteins by fluorescence microscopy and flow cytometry. Texas Red's red emission pairs cleanly with green fluorophores for dual labeling. Supplied as 2 mg in liquid. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Concanavalin A Lectin (Con A) – Texas Red – Catalog #B2025513

Concanavalin A – Texas Red (Catalog #B2025513) combines the carbohydrate specificity of Concanavalin A, the legume lectin from jack bean (Canavalia ensiformis), with the red fluorescence of Texas Red. Supplied as 2 mg in liquid form, it is used to label and visualize mannose- and glucose-containing glycoproteins and glycolipids on cells and tissue sections.

Catalog number: B2025513
Lot number: Batch dependent
Expiration Date: Batch dependent
Amount: 2 mg
Molecular Weight or Concentration: N/A
Supplied as: Liquid
Source: Jack bean (Canavalia ensiformis)
Label: Texas Red (Ex ~589 nm / Em ~615 nm)
Carbohydrate specificity: α-D-mannosyl and α-D-glucosyl residues
Applications: Fluorescence microscopy, flow cytometry, cell-surface glycoconjugate labeling, dual-color labeling with green fluorophores
Storage: 2–8°C, protected from light
Keywords: Concanavalin A, Con A, ConA Texas Red, Texas Red Concanavalin A, Texas Red lectin, fluorescent Con A, Canavalia ensiformis lectin, jack bean lectin, mannose-binding lectin
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

Scientific Overview

Concanavalin A is one of the most thoroughly characterized lectins. It recognizes terminal and internal α-D-mannosyl and α-D-glucosyl residues and binds the trimannoside core common to N-linked glycans with high affinity; crystal structures of Con A–trimannoside complexes show how all three sugar residues are held in an extended binding cleft. Carbohydrate binding depends on bound manganese and calcium ions.

Texas Red (sulforhodamine 101 derivative) was introduced as a hydrophilic, red-emitting fluorophore designed to be used alongside fluorescein in dual-parameter flow cytometry and fluorescence microscopy. Its emission near 615 nm is well separated from green fluorophores, so Con A – Texas Red can be combined with FITC- or GFP-labeled probes.

Key applications include:

  • Fluorescent staining of cell-surface glycoproteins and glycolipids
  • Flow cytometric analysis of glycoconjugate expression
  • Visualizing glycoprotein distribution in cells and tissue sections
  • Dual-color labeling with green-emitting probes

Usage & Handling Guidance

Store at 2–8°C and protect from light. Titrate the conjugate for each cell type or tissue to find the concentration that gives the best signal-to-background ratio.

  • Buffers: Avoid EDTA, EGTA, and other chelators, which strip the metal ions Con A needs for sugar binding.
  • Specificity control: Pre-incubate the lectin with a competing sugar such as methyl α-D-mannopyranoside; loss of staining confirms carbohydrate-specific binding.
  • Imaging: Use filter sets suited to Texas Red (excitation ~590 nm, emission ~615 nm).

What You Get

  • 2 mg of Concanavalin A conjugated to Texas Red, supplied as liquid
  • A well-characterized mannose/glucose-specific lectin probe
  • Red fluorescence suited to multicolor experiments
  • For research use only (RUO)

Why Researchers Choose It

  • Extensively characterized carbohydrate specificity backed by crystal structures
  • Bright red emission that separates cleanly from green channels
  • Works in both imaging and flow cytometry workflows
  • Simple competition control with methyl α-D-mannopyranoside

Frequently Asked Questions (FAQ)

  • What does Concanavalin A bind?
    α-D-mannosyl and α-D-glucosyl residues, including the trimannoside core found in N-linked glycans.
  • What are the excitation and emission maxima of Texas Red?
    Approximately 589 nm excitation and 615 nm emission.
  • Can I combine it with FITC or GFP?
    Yes. Texas Red was developed for use alongside fluorescein, and its emission is well separated from green fluorophores.
  • Why is my staining weak?
    Check that your buffers contain no chelators such as EDTA, since Con A requires bound metal ions to bind sugars, and titrate the conjugate concentration.
  • How do I confirm the staining is specific?
    Run a control with a competing sugar such as methyl α-D-mannopyranoside; specific staining should be strongly reduced.
This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use in humans or animals.

References

  • Naismith JH, Field RA. Structural basis of trimannoside recognition by concanavalin A. J Biol Chem. 1996;271(2):972-976.Reference
  • Loris R, Maes D, Poortmans F, Wyns L, Bouckaert J. A structure of the complex between concanavalin A and methyl-3,6-di-O-(α-D-mannopyranosyl)-α-D-mannopyranoside reveals two binding modes. J Biol Chem. 1996;271(48):30614-30618.Reference
  • Titus JA, Haugland R, Sharrow SO, Segal DM. Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies. J Immunol Methods. 1982;50(2):193-204.Reference

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