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Animal Free Collagenase Type B Proteins Molecular Depot
Animal Free Collagenase Type B Proteins Molecular Depot
Animal Free Collagenase Type B Proteins Molecular Depot
Animal Free Collagenase Type B Proteins Molecular Depot

Animal Free Collagenase Type B

$517.00

    Catalog Number: B2012566 (25 mg)

    Animal Free Collagenase Type B is a high quality animal-free Collagenase type B. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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    Email: info@bluetigerscientific.com

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VR Headset

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2 Touch Controllers

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Charging Cable

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Animal Free Collagenase Type B – Catalog #B2012566 (25 mg)

Catalog # B2012566
Lot # Batch dependent
Expiration Batch dependent
Amount 25 mg
Molecular Capacity 300 units/mg
Supplied As Lyophilized Powder
Applications Molecular tool for a wide array of biochemical and immunological applications
Storage –20 °C
Grade Biotechnology grade; highly pure; prepared in Type I ultrapure water (resistivity >18 MΩ·cm) and 0.22 µm filtered
Keywords Bacteria-Derived Animal Free Collagenase Type B

Premium animal-free collagenase designed for precise extracellular matrix degradation and sensitive research workflows. Animal Free Collagenase Type B (Catalog #B2012566, 25 mg) is a powerful, bacteria-derived proteolytic enzyme formulated without any animal components—ideal for rigorous biochemical and immunological applications demanding uncompromised purity. With a defined activity of approximately 300 units per mg, this lyophilized powder provides consistent catalytic performance across experimental setups. Its animal-free nature also significantly reduces the risk of xenogeneic contaminants, enhancing assay fidelity in translational, regenerative, and therapeutic research contexts.

Engineered for controlled collagen degradation, this enzyme is particularly well-suited for dense tissue dissociation, matrix remodeling, and sample prep in proteomics workflows. The lyophilized format ensures long-term stability and flexibility in dosing, while the biotechnology-grade production process—employing ultrapure water and sterile filtration—supports lot-to-lot reproducibility. This makes Animal Free Collagenase Type B an optimal choice across academic and industrial RUO environments, such as organoid development, ECM degradation studies, or standardized enzyme activity assays.

Labs conducting sensitive translational experiments or regulatory-sensitive workflows will appreciate the animal-free designation, enabling clearer downstream data interpretation and minimizing concerns over animal-derived impurities. Bulk custom orders are available to accommodate scaling needs, ensuring uninterrupted access for long-term projects. Whether used for histological matrix clearance, cell isolate preparation, or method validation protocols, this collagenase delivers dependable precision and consistency across diverse research pipelines.

Why researchers choose this product:

  • Animal-free formulation mitigates risks of animal-derived contaminants and immunological interference
  • High enzymatic activity (300 units/mg) ensures efficient collagen degradation
  • Lyophilized powder offers robust stability and flexible usage
  • Biotech-grade manufacturing supports stringent RUO reproducibility
  • Custom bulk supply supports scalability and continuity in research workflows

Indication for Use:
This enzyme is for Research Use Only (RUO). It is not intended for clinical, diagnostic, or therapeutic use.

References:

  • 1: Duan S, Wang F, Cao J, Wang C. Exosomes Derived from MicroRNA-146a-5p-Enriched Bone Marrow Mesenchymal Stem Cells Alleviate Intracerebral Hemorrhage by Inhibiting Neuronal Apoptosis and Microglial M1 Polarization. Drug Des Devel Ther. 2020 Aug 5;14:3143-3158.
  • 2: Wucherpfennig AL, Li YP, Stetler-Stevenson WG, Rosenberg AE, Stashenko P. Expression of 92 kD type IV collagenase/gelatinase B in human osteoclasts. J Bone Miner Res. 1994 Apr;9(4):549-56.
  • 3: Chen S, So EC, Strome SE, Zhang X. Impact of Detachment Methods on M2 Macrophage Phenotype and Function. J Immunol Methods. 2015 Nov;426:56-61.
  • 4: Sires UI, Schmid TM, Fliszar CJ, Wang ZQ, Gluck SL, Welgus HG. Complete degradation of type X collagen requires the combined action of interstitial collagenase and osteoclast-derived cathepsin-B. J Clin Invest. 1995 May;95(5):2089-95.
  • 5: Leber TM, Balkwill FR. Zymography: a single-step staining method for quantitation of proteolytic activity on substrate gels. Anal Biochem. 1997 Jun 15;249(1):24-8.
  • 6: Mohtai M, Smith RL, Schurman DJ, Tsuji Y, Torti FM, Hutchinson NI, Stetler-Stevenson WG, Goldberg GI. Expression of 92-kD type IV collagenase/gelatinase (gelatinase B) in osteoarthritic cartilage and its induction in normal human articular cartilage by interleukin 1. J Clin Invest. 1993 Jul;92(1):179-85.
  • 7: Muscella A, Vetrugno C, Cossa LG, Marsigliante S. TGF-β1 activates RSC96 Schwann cells migration and invasion through MMP-2 and MMP-9 activities. J Neurochem. 2020 May;153(4):525-538.
  • 8: Miow QH, Vallejo AF, Wang Y, Hong JM, Bai C, Teo FS, Wang AD, Loh HR, Tan TZ, Ding Y, She HW, Gan SH, Paton NI, Lum J, Tay A, Chee CB, Tambyah PA, Polak ME, Wang YT, Singhal A, Elkington PT, Friedland JS, Ong CW. Doxycycline host-directed therapy in human pulmonary tuberculosis. J Clin Invest. 2021 Aug 2;131(15):e141895.
  • 9: Sangiorgi G, Trimarchi S, Mauriello A, Righini P, Bossone E, Suzuki T, Rampoldi V, Eagle KA. Plasma levels of metalloproteinases-9 and -2 in the acute and subacute phases of type A and type B aortic dissection. J Cardiovasc Med (Hagerstown). 2006 May;7(5):307-15.
  • 10: Weeks BS, Schnaper HW, Handy M, Holloway E, Kleinman HK. Human T lymphocytes synthesize the 92 kDa type IV collagenase (gelatinase B). J Cell Physiol. 1993 Dec;157(3):644-9.

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    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

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    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
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    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

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    Governing Law
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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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