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Animal Free Collagenase Type A Proteins Molecular Depot
Animal Free Collagenase Type A Proteins Molecular Depot
Animal Free Collagenase Type A Proteins Molecular Depot
Animal Free Collagenase Type A Proteins Molecular Depot

Animal Free Collagenase Type A

$517.00

    Catalog Number: B2013165 (25 mg)

    Animal Free Collagenase Type A is a high quality bacteria-produced animal free Collagenase Type A. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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    Email: info@bluetigerscientific.com

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VR Headset

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2 Touch Controllers

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Animal Free Collagenase Type A – Catalog #B2013165 (25 mg)

Catalog # B2013165
Lot # Batch dependent
Expiration Batch dependent
Amount 25 mg
Molecular Capacity 150 units/mg
Supplied As Lyophilized Powder
Applications Molecular tool for diverse biochemical and immunological applications
Storage –20 °C
Grade Biotechnology grade; highly pure; prepared with Type I ultrapure water (>18 MΩ·cm); 0.22 µm filtered
Keywords Bacteria-Derived Animal Free Collagenase Type A

High-integrity, animal-free collagenase tailored for rigorous ECM digestion and cell isolation workflows. Animal Free Collagenase Type A (Catalog #B2013165, 25 mg) combines research-grade enzymatic integrity with a formulation completely devoid of animal-derived components—making it ideal for sensitive and translational laboratory applications. With a defined activity of 150 units/mg, this lyophilized powder delivers precise and robust breakdown of collagen substrates derived from *Clostridium histolyticum*.

This enzyme is embraced by researchers for its dual functionality: it offers collagenase activity akin to Type I and II preparations while eliminating risks associated with mammalian contaminants—critical for regenerative medicine assays, stem cell workflows, and regulatory-sensitive studies. Its lyophilized format enhances durability and simplifies dosing, while the biotech-grade purification process ensures low background interference and reproducibility across experimental batches.

Whether used in histopathological protocols, matrix remodeling studies, or proteomic sample preparations, Animal Free Collagenase Type A excels as a dependable molecular tool. Researchers value its traceable quality, compatibility with high-throughput workflows, and suitability for contexts requiring the highest standards of purity. Custom bulk quantities are available, supporting seamless integration into scaling projects and long-term pipeline strategies.

Why researchers choose this product:

  • Animal-free formulation eliminates mammalian component risks and simplifies regulatory validation
  • High-purity, biotech-grade enzyme ensures minimal background and workflow consistency
  • Defined activity (150 units/mg) balances performance and dosing control
  • Lyophilized powder format offers stability and flexibility across uses
  • Well-suited for regenerative medicine, proteomics, and translational research workflows

Indication for Use:
This product is for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or clinical applications.

References:

  • Brandhorst D, Parnaud G, Friberg A, Lavallard V, Demuylder-Mischler S, Hughes S, Saphörster J, Kurfürst M, Korsgren O, Berney T, Johnson PRV. Multicenter Assessment of Animal-free Collagenase AF-1 for Human Islet Isolation Cell Transplant. 2017 Oct;26(10):1688-1693.
  • Stetler-Stevenson WG, Talano JA, Gallagher ME, Krutzsch HC, Liotta LA. Inhibition of human type IV collagenase by a highly conserved peptide sequence derived from its prosegment Am J Med Sci. 1991 Sep;302(3):163-70.
  • Vaithilingam V, Sundaram G, Tuch BE. Islet cell transplantation Curr Opin Organ Transplant. 2008 Dec;13(6):633-8.
  • Aimes RT, Quigley JP. Matrix metalloproteinase-2 is an interstitial collagenase. Inhibitor-free enzyme catalyzes the cleavage of collagen fibrils and soluble native type I collagen generating the specific 3/4- and 1/4-length fragments J Biol Chem. 1995 Mar 17;270(11):5872-6.
  • Kamata H, Ashikari-Hada S, Mori Y, Azuma A, Hata KI. Extemporaneous Preparation of Injectable and Enzymatically Degradable 3D Cell Culture Matrices from an Animal-Component-Free Recombinant Protein Based on Human Collagen Type I Macromol Rapid Commun. 2019 Aug;40(15):e1900127.
  • Mou L, Shi G, Cooper DKC, Lu Y, Chen J, Zhu S, Deng J, Huang Y, Ni Y, Zhan Y, Cai Z, Pu Z. Current Topics of Relevance to the Xenotransplantation of Free Pig Islets Front Immunol. 2022 Apr 1;13:854883.
  • Teale DM, Khidair IA, Potter CW, Rees RC. Modulation of type IV collagenase and plasminogen activator in a hamster fibrosarcoma by basement membrane components and lung fibroblasts Br J Cancer. 1988 May;57(5):475-80.
  • Villegas MR, Baeza A, Usategui A, Ortiz-Romero PL, Pablos JL, Vallet-Regí M. Collagenase nanocapsules: An approach to fibrosis treatment Acta Biomater. 2018 Jul 1;74:430-438.
  • Sawaya R, Tofilon PJ, Mohanam S, Ali-Osman F, Liotta LA, Stetler-Stevenson WG, Rao JS. Induction of tissue-type plasminogen activator and 72-kDa type-IV collagenase by ionizing radiation in rat astrocytes Int J Cancer. 1994 Jan 15;56(2):214-8.

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    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

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    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

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    Quality System (MD Levels)
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    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

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    Intellectual Property
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    Force Majeure
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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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