alpha-Endorphin Antibody Conjugated to Biotin – Research Use Only

alpha-Endorphin Antibody Conjugated to Biotin is a biotinylated polyclonal antibody specific for α-endorphin, an endogenous opioid peptide derived from pro-opiomelanocortin (POMC). Supplied as a 50 µg solution, this high-affinity biotin conjugate enables sensitive streptavidin-based detection of α-endorphin in immunoassays, immunohistochemistry, and neuroendocrine research.

Scientific Overview

α-Endorphin is an endogenous opioid peptide generated from the precursor pro-opiomelanocortin (POMC). It exhibits opioid activity primarily through μ- and δ-opioid receptors and is involved in pain modulation, stress response, and neuroendocrine regulation. The biotinylated antibody allows high-sensitivity detection and localization of α-endorphin in neural tissues, plasma, and other biological samples using streptavidin-based amplification systems.

This alpha-Endorphin Antibody Biotin Conjugate is suitable for:

• Immunohistochemical mapping of α-endorphin in the central nervous system
• Quantification of α-endorphin by ELISA or radioimmunoassay
• Studies of opioid peptide systems, stress axis, and pain pathways
• Investigation of endorphin immunoreactivity in physiological and pathological conditions
• Neuropeptide co-localization and neuroendocrine research

Usage & Handling Guidance

Store the antibody solution at -20 °C. Avoid repeated freeze–thaw cycles by preparing single-use working aliquots. Thaw gently on ice and mix by gentle inversion before use. Dilute in buffer containing carrier protein (e.g., 1% BSA) immediately prior to assay.

• Recommended applications: ELISA, RIA, IHC, and streptavidin-biotin detection systems
• Working dilution: Optimize empirically for each tissue, fixation method, and detection platform
• Detection: Pair with streptavidin-HRP, streptavidin-AP, or streptavidin-fluorophores
• Fixation: Compatible with aldehyde-based fixatives for IHC (optimize epitope preservation as needed)

What You Get

• 50 µg alpha-Endorphin Antibody conjugated to Biotin in solution
• High-affinity biotinylated reagent for sensitive streptavidin-based detection
• Biotechnology-grade antibody suitable for opioid peptide and neuroendocrine research
• Batch-specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Specific recognition of α-endorphin (alpha-endorphin)
• Biotin conjugation enables flexible, high-sensitivity streptavidin amplification
• Excellent for mapping endorphin distribution and quantifying opioid peptides
• Supports studies in pain modulation, stress response, and neuroendocrine signaling
• High-purity biotechnology-grade formulation

Frequently Asked Questions (FAQ)

• What does this antibody specifically detect?
  It is directed against α-endorphin (alpha-endorphin), an endogenous opioid peptide derived from POMC.
• What detection systems are compatible?
  The biotin tag allows pairing with any streptavidin-conjugated reporter (HRP, AP, fluorophores, or magnetic beads).
• What is the recommended storage condition?
  Store at -20 °C. Prepare aliquots to avoid repeated freeze–thaw cycles.
• Is this antibody suitable for immunohistochemistry?
  Yes. It is commonly used for IHC to visualize α-endorphin in brain sections and neuroendocrine tissues.
• Can it be used for quantification of α-endorphin?
  Yes, when combined with streptavidin-HRP in ELISA or radioimmunoassay formats.

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References

• Bialka S, Sliwczynska M, Marciniak A, Czyzewski D, Misiolek H. An assessment of the effectiveness of regional analgesia after VATS measured by an objective method for assessing testosterone, cortisol, α-amylase, sIgA, and β-endorphin levels – a randomised controlled trial Endokrynol Pol. 2021;72(2):133-142.
• Nagahama M, Funasaka Y, Fernandez-Frez ML, Ohashi A, Chakraborty AK, Ueda M, Ichihashi M. Immunoreactivity of alpha-melanocyte-stimulating hormone, adrenocorticotrophic hormone and beta-endorphin in cutaneous malignant melanoma and benign melanocytic naevi Br J Dermatol. 1998 Jun;138(6):981-5.
• Wu HY, Tang XQ, Mao XF, Wang YX. Autocrine Interleukin-10 Mediates Glucagon-Like Peptide-1 Receptor-Induced Spinal Microglial β-Endorphin Expression J Neurosci. 2017 Nov 29;37(48):11701-11714.
• Weber E, Barchas JD. Immunohistochemical distribution of dynorphin B in rat brain: relation to dynorphin A and alpha-neo-endorphin systems Proc Natl Acad Sci U S A. 1983 Feb;80(4):1125-9.
• Gramsch C, Meo T, Riethmüller G, Herz A. Binding characteristics of a monoclonal beta-endorphin antibody recognizing the N-terminus of opioid peptides J Neurochem. 1983 May;40(5):1220-6.
• Winther Bach F, Ekman R, Jensen FM. Beta-endorphin-immunoreactive components in human cerebrospinal fluid Regul Pept. 1986 Dec 30;16(3-4):189-98.
• Johnson HM, Smith EM, Torres BA, Blalock JE. Regulation of the in vitro antibody response by neuroendocrine hormones Proc Natl Acad Sci U S A. 1982 Jul;79(13):4171-4.
• Berczi I, Chalmers IM, Nagy E, Warrington RJ. The immune effects of neuropeptides Baillieres Clin Rheumatol. 1996 May;10(2):227-57.
• Liu H, He Y, Beck J, da Silva Teixeira S, Harrison K, Xu Y, Sisley S. Defining vitamin D receptor expression in the brain using a novel VDR(Cre) mouse J Comp Neurol. 2021 Jun;529(9):2362-2375.