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360 MMP FRET Substrate VI Chemicals Molecular Depot
360 MMP FRET Substrate VI Chemicals Molecular Depot
360 MMP FRET Substrate VI Chemicals Molecular Depot
360 MMP FRET Substrate VI Chemicals Molecular Depot

360 MMP FRET Substrate VI

$795.00

    Catalog Number: B2019483 (2 mg)

    360 MMP FRET Substrate VI is a biochemical substrate used in fluorescence resonance energy transfer (FRET) assays to study matrix metalloproteinases (MMPs). The substrate is designed to be non-fluorescent until cleaved by MMPs, resulting in a measurable increase in fluorescence, allowing researchers to quantify MMP activity. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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What's Included

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VR Headset

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2 Touch Controllers

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Charging Cable

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Power Adapter

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Glasses Spacer

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360 MMP FRET Substrate VI

Catalog number: B2019483
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 2 mg
Molecular Weight or Concentration: 1042.1
Supplied as: Powder
Applications: a molecular tool for various biochemical applications
Storage: -20°C
Keywords: Dnp-PLAYWAR
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References

  • Abid Ali F, Renault L, Gannon J, Gahlon HL, Kotecha A, Zhou JC, Rueda D, Costa A. Cryo-EM structures of the eukaryotic replicative helicase bound to a translocation substrate Nat Commun. 2016 Feb 18;7:10708.
  • Noble E, Cox A, Deval J, Kim B. Endonuclease substrate selectivity characterized with full-length PA of influenza A virus polymerase Virology. 2012 Nov 10;433(1):27-34.
  • Aggarwal M, Sharma R, Kumar P, Parida M, Tomar S. Kinetic characterization of trans-proteolytic activity of Chikungunya virus capsid protease and development of a FRET-based HTS assay Sci Rep. 2015 Oct 6;5:14753.
  • Ihssen J, Faccio G, Yao C, Sirec T, Spitz U. Fluorogenic in vitro activity assay for the main protease M(pro) from SARS-CoV-2 and its adaptation to the identification of inhibitors STAR Protoc. 2021 Sep 17;2(3):100793.
  • Hu Y, Wen HY, Liu MY, Wang JM, Dong RL, Liu SL, Wang ZG. In Situ Quantitative Imaging of Plasma Membrane Stiffness in Live Cells Using a Genetically Encoded FRET Sensor Anal Chem. 2024 May 28;96(21):8501-8509.
  • Jian-rui L, Yan-bin W, Shu-yi S, Hong-shan C, Jian-dong J, Zong-gen B. Establishment and application of high throughput screening model for hepatitis C virus NS3-4A protease inhibitors in vitro Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2011 Feb;33(1):98-101.
  • Emmott E, Sweeney TR, Goodfellow I. A Cell-based Fluorescence Resonance Energy Transfer (FRET) Sensor Reveals Inter- and Intragenogroup Variations in Norovirus Protease Activity and Polyprotein Cleavage J Biol Chem. 2015 Nov 13;290(46):27841-53.
  • Xu S, Ci Y, Wang L, Yang Y, Zhang L, Xu C, Qin C, Shi L. Zika virus NS3 is a canonical RNA helicase stimulated by NS5 RNA polymerase Nucleic Acids Res. 2019 Sep 19;47(16):8693-8707.
  • Theerawatanasirikul S, Thangthamniyom N, Kuo CJ, Semkum P, Phecharat N, Chankeeree P, Lekcharoensuk P. Natural Phytochemicals, Luteolin and Isoginkgetin, Inhibit 3C Protease and Infection of FMDV, In Silico and In Vitro Viruses. 2021 Oct 21;13(11):2118.

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Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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