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25-OH Vitamin D3 Biotin Conjugate Chemicals Molecular Depot
25-OH Vitamin D3 Biotin Conjugate Chemicals Molecular Depot
25-OH Vitamin D3 Biotin Conjugate Chemicals Molecular Depot
25-OH Vitamin D3 Biotin Conjugate Chemicals Molecular Depot

25-OH Vitamin D3 Biotin Conjugate

$1,695.00

    Catalog Number: B2010192 (1 mg)

    25-OH Vitamin D3 Biotin Conjugate consists of 25-OH Vitamin D3 conjugated to Biotin via a 22 A spacer arm. The spacer adds flexibility to the 25-OH Vitamin D3 moiety which is required for its interaction with specific antibodies and other binding partners. This compound has been validated for use in several 25-OH Vitamin D applications including ELISA. The conjugate is supplied as a DMSO stock solution. Custom bulk orders of this product are available upon request.

    Products are for in vitro research use only (RUO).

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25-OH Vitamin D3 Biotin Conjugate

Catalog number: B2010192
Lot number: Batch Dependent
Expiration Date: Batch Dependent
Amount: 1 mg
Molecular Weight: 797 g/mol
Appearance: Solution
Concentration: 1 mg/mL in DMSO (resuspend first in 100% Ethanol or other buffers to working concentrations, not directly soluble in water)
Purity: >97%
Storage: −20°C
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 μm.
Other names: 25-OH Vitamin D3-LC-Biotin, 25-OH Vitamin D3 LC-Biotin, Synthetic 25-OH Vitamin D3 LC Biotin, 25-OH Vitamin D3-LC-Biotin, Synthetic 25-OH Vitamin D3 LC-Biotin, Human Synthetic 25-OH Vitamin D3 LC Biotin, N-(3-(((1S,Z)-3-(2-((1R,7aR,E)-1-((R)-6-hydroxy-6-methylheptan-2-yl)-7a-methylhexahydro-1H-inden-4(2H)-ylidene)ethylidene)-4-methylenecyclohexyl)oxy)propyl)-6-(5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamide
Keywords: 25-OH Vitamin D3-LC-Biotin, 25-OH Vitamin D3 LC-Biotin, Synthetic 25-OH Vitamin D3 LC Biotin, Human Synthetic 25-OH Vitamin D3 LC Biotin

This biotechnology-grade conjugate links 25-OH Vitamin D3 to biotin via a 22 Å spacer arm, enhancing flexibility and accessibility for antibody recognition. The long linker improves binding efficiency in ELISA and other immunoassay formats. Supplied at 1 mg/mL in DMSO, it must be resuspended in ethanol or compatible buffers before use. It is not directly soluble in water.

Validated for use in 25-OH Vitamin D3 detection workflows, this reagent supports applications in nutritional diagnostics, vitamin D metabolism studies, and immunoassay calibration. All components meet ≥99% purity standards and are prepared using ultrapure water and sterile filtration.

Key benefits:

  • Biotinylated 25-OH Vitamin D3 with 22 Å spacer for enhanced antibody interaction
  • Supplied at 1 mg/mL in DMSO; resuspend in ethanol or buffer
  • High purity (>97%) and biotechnology-grade formulation
  • Validated for ELISA and vitamin D3 detection assays
  • Stable at −20°C; avoid repeated freeze-thaw

Products are for in vitro research use only (RUO).

References

  • Holick MF, Smith E, Pincus S. Skin as the site of vitamin D synthesis and target tissue for 1,25-dihydroxyvitamin D3. Use of calcitriol (1,25-dihydroxyvitamin D3) for treatment of psoriasis. Arch Dermatol. 1987 Dec;123(12):1677–1683a.
  • Guo J, Lovegrove JA, Givens DI. 25(OH)D3-enriched or fortified foods are more efficient at tackling inadequate vitamin D status than vitamin D3. Proc Nutr Soc. 2018 Aug;77(3):282–291. doi: 10.1017/S0029665117004062.
  • Hassan AB, Hozayen RF, Alotaibi RA, Tayem YI. Therapeutic and maintenance regimens of vitamin D3 supplementation in healthy adults: A systematic review. Cell Mol Biol (Noisy-le-grand). 2018 Nov 30;64(14):8–14.
  • McCullough PJ, Lehrer DS, Amend J. Daily oral dosing of vitamin D3 using 5000 TO 50,000 international units a day in long-term hospitalized patients: Insights from a seven year experience. J Steroid Biochem Mol Biol. 2019 May;189:228–239.
  • Rosendahl J, Valkama S, Holmlund-Suila E, Enlund-Cerullo M, Hauta-Alus H, Helve O, Hytinantti T, Levälahti E, Kajantie E, Viljakainen H, Mäkitie O, Andersson S. Effect of Higher vs Standard Dosage of Vitamin D3 Supplementation on Bone Strength and Infection in Healthy Infants: A Randomized Clinical Trial. JAMA Pediatr. 2018 Jul 1;172(7):646–654.
  • Penagini F, Borsani B, Maruca K, Giosia V, Bova S, Mastrangelo M, Zuccotti GV, Mora S. Short-Term Vitamin D3 Supplementation in Children with Neurodisabilities: Comparison of Two Delivery Methods. Horm Res Paediatr. 2017;88(3–4):281–284.

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FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
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Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
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