17a Trenbolone Conjugated to HRP – Research Use Only

Scientific Overview

17a Trenbolone Conjugated to HRP is a specialized research reagent designed for sensitive and selective detection of trenbolone in immunoassay formats. Trenbolone, a synthetic androgen, is widely studied in environmental toxicology, endocrine disruption research, and veterinary science. Conjugation to Horseradish Peroxidase (HRP) enables robust signal amplification, making this reagent ideal for competitive ELISA development and quantitative detection workflows.

Common Research Applications

• Competitive ELISA development for trenbolone detection
• Environmental monitoring of androgenic contaminants
• Biomonitoring studies involving steroidal pollutants
• Assay development for endocrine disruption research
• Studies of steroid metabolism and transformation pathways

Usage & Handling Guidance

This HRP conjugate is supplied as a ready-to-use solution. For ELISA and immunoassay workflows, researchers typically dilute the conjugate in assay buffer (e.g., PBS with BSA or casein). Gentle mixing is recommended to preserve enzymatic activity. Avoid repeated freeze–thaw cycles by aliquoting upon receipt.

• Recommended storage: −20 °C
• Thawing: thaw on ice and mix gently
• Avoid: repeated freeze–thaw cycles and prolonged exposure to room temperature

Research Background

Trenbolone and its metabolites are frequently detected in agricultural runoff and aquatic environments, where they can exert potent androgenic effects. HRP-conjugated trenbolone derivatives are widely used in immunoassay-based detection systems to quantify trace levels of trenbolone in water, sediment, and biological samples. Published studies highlight trenbolone’s environmental persistence, phototransformation behavior, and endocrine-disrupting potential, making sensitive analytical tools essential for monitoring and research.

What You Get

• 500 µL of 17a Trenbolone–HRP conjugate
• Biotechnology-grade solution
• Sterile-filtered (0.22 µm)
• Prepared using Type I ultrapure water
• For research use only (RUO)

Frequently Asked Questions

• Is this product suitable for clinical or diagnostic use? No. This product is for research use only.
• Can I obtain a Certificate of Analysis (COA) or Technical Data Sheet (TDS)? COA and TDS availability depends on the manufacturer’s current active batch. In many cases, documentation can be provided upon request. Please contact us to inquire about documentation for the specific lot being shipped.
• What buffers are recommended for dilution? Researchers commonly use PBS, TBS, or assay-specific buffers containing BSA or casein.
• Is this conjugate compatible with competitive ELISA formats? Yes. HRP-conjugated steroids are widely used in competitive immunoassays.

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References

• Ankley GT, Coady KK, Gross M, Holbech H, Levine SL, Maack G, Williams M. A critical review of the environmental occurrence and potential effects in aquatic vertebrates of the potent androgen receptor agonist 17β-trenbolone. Environ Toxicol Chem. 2018 Aug;37(8):2064-2078.
• Zhang S, Jiao Z, Zhao X, Sun M, Feng X. Environmental exposure to 17β-trenbolone during adolescence inhibits social interaction in male mice. Environ Pollut. 2021 Nov 15;289:117710.
• Yang X, Zhao H, Cwiertny DM, Kolodziej EP. Sorption and transport of trenbolone and altrenogest photoproducts in soil-water systems. Environ Sci Process Impacts. 2019 Oct 16;21(10):1650-1663.
• Tan H, Bertram MG, Martin JM, Ecker TE, Hannington SL, Saaristo M, O’Bryan MK, Wong BBM. The endocrine disruptor 17β-trenbolone alters the relationship between pre- and post-copulatory sexual traits in male mosquitofish (Gambusia holbrooki). Sci Total Environ. 2021 Oct 10;790:148028.
• Robinson JA, Ma Q, Staveley JP, Smolenski WJ. Sorption and desorption of 17α-trenbolone and trendione on five soils. Environ Toxicol Chem. 2017 Mar;36(3):613-620.
• Robinson JA, Ma Q, Staveley JP, Smolenski WJ, Ericson J. Degradation and transformation of 17α-trenbolone in aerobic water-sediment systems. Environ Toxicol Chem. 2017 Mar;36(3):630-635.
• Zhang S, Zhang S, Zhu D, Jiao Z, Zhao X, Sun M, Che Y, Feng X. Effects of 17β-trenbolone exposure on sex hormone synthesis and social behaviours in adolescent mice. Chemosphere. 2020 Apr;245:125679.
• Pflug NC, Kral AK, Hankard MK, Breuckman KC, Kolodziej EP, Gloer JB, Wammer KH, Cwiertny DM. Photolysis of Trenbolone Acetate Metabolites in the Presence of Nucleophiles: Evidence for Metastable Photoaddition Products and Reversible Associations with Dissolved Organic Matter. Environ Sci Technol. 2020 Oct 6;54(19):12181-12190.
• Mizukami-Murata S, Kishi-Kadota K, Nishida T. 17β-Trenbolone exposure programs metabolic dysfunction in larval medaka. Environ Toxicol. 2016 Nov;31(11):1539-1551.