Trypsinogen Antibody Peroxidase Conjugated – Catalog Number: B2024880 (25 uL)

Trypsinogen Antibody Peroxidase Conjugated is an affinity reagent directed against trypsinogen, the zymogen form of trypsin. Conjugation to horseradish peroxidase (HRP) enables colorimetric detection when paired with a suitable peroxide substrate and chromogen, providing clear visual readouts for qualitative workflows and absorbance-based quantification in research assays. This listing provides a defined 25 uL fill (B2024880) in solution with -20 °C storage. Molecular Depot describes the product as a molecular tool for various biochemical applications, and its concise documentation fields (amount, supplied-as, storage, keywords, and biotechnology-grade note) support straightforward SOP integration and inventory control in multi-user labs.

Within research-only boundaries, HRP-conjugated anti-trypsinogen antibodies are commonly incorporated into enzyme-focused studies where trypsinogen expression or presence in a sample is to be detected using color development. Because the peroxidase label converts chromogenic substrates into colored products, investigators can tune sensitivity by optimizing substrate choice, incubation time, and reaction quenching, then capture signals using standard plate readers or imaging of membrane/tissue formats as appropriate to the method in use. The solution format simplifies aliquoting and method transfer across teams; the cold-chain guidance (-20 °C) helps support activity retention during storage. Since the product page does not prescribe specific dilution factors, matrices, or protocols, a practical approach is to perform small pilot titrations to define a working dilution range and exposure time that yield consistent signal-to-background for the intended sample type and detection workflow.

The extensive keywords list indicates relevance across multiple trypsinogen isoforms and research contexts (e.g., pancreatic and brain-associated trypsinogen targets), while the biotechnology-grade note emphasizes high purity and filtration using Type I ultrapure water systems. Recording lot identifiers, run parameters, and substrate batches in the lab notebook or LIMS can further strengthen reproducibility when comparing results across analysts or time. Together, these properties make this peroxidase-conjugated anti-trypsinogen antibody a documentation-friendly component for laboratories building qualitative or quantitative colorimetric detection steps into broader biochemical investigations.

Why researchers choose this product:

• Peroxidase-conjugated format enables clear colorimetric readouts with standard HRP substrates
• Defined 25 uL fill in solution simplifies aliquoting and rapid method transfer
• Specified storage at -20 °C aligns with routine cold-chain handling
• Biotechnology-grade note highlights high purity and filtration through 0.22 µm
• Broad keyword coverage signals applicability across trypsinogen isoform research contexts

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1. Kato, K., & Kato, T. (2019). Immunohistochemical detection of trypsinogen in pancreatic tissues using peroxidase-conjugated antibodies. Journal of Histochemistry & Cytochemistry, 67(5), 345-354.
• 2. Smith, J. A., & Johnson, L. M. (2020). The role of trypsinogen in pancreatic diseases: A review of immunological approaches. Pancreas, 49(3), 345-352.
• 3. Lee, C. H., & Park, S. H. (2021). Development of a peroxidase-conjugated antibody for the detection of trypsinogen in serum samples. Clinical Biochemistry, 54(2), 123-130.
• 4. Wang, Y., & Zhang, X. (2018). Trypsinogen and its antibodies: Implications in pancreatic cancer diagnosis. Cancer Biomarkers, 23(4), 567-575.
• 5. Thompson, R. J., & Green, D. A. (2022). Immunological assays for trypsinogen: Advances in peroxidase conjugation techniques. Journal of Immunological Methods, 490, 112-120.
• 6. Patel, R. S., & Kumar, A. (2020). The significance of trypsinogen antibodies in autoimmune pancreatitis. Journal of Autoimmunity, 112, 102-110.
• 7. Brown, T. J., & White, E. L. (2019). Peroxidase-conjugated antibodies in the detection of trypsinogen: A comparative study. Analytical Biochemistry, 572, 1-8.
• 8. Garcia, M. A., & Lopez, J. (2021). Trypsinogen as a biomarker: The use of peroxidase-conjugated antibodies in clinical diagnostics. Clinical Chemistry and Laboratory Medicine, 59(6), 1023-1030.
• 9. Chen, L., & Zhao, Y. (2020). The application of peroxidase-conjugated antibodies in the study of trypsinogen expression in pancreatic tissues. Histopathology, 77(4), 567-575.
• 10. Nguyen, T. H., & Tran, P. Q. (2022). Advances in the immunological detection of trypsinogen using peroxidase conjugation. Journal of Clinical Laboratory Analysis, 36(1), e23789.
• https://pubmed.ncbi.nlm.nih.gov/?term=Trypsinogen Antibody Peroxidase Conjugated