Sucrose Phosphorylase – Catalog Number: B2020184 (1 kU)

Sucrose phosphorylase catalyzes the reversible conversion of sucrose and inorganic phosphate (Pi) to yield glucose-1-phosphate and fructose. This listing provides a defined 1 kU fill (Catalog B2020184) supplied as a lyophilized preparation with a listed molecular-weight entry of 56 kDa and −20 C storage. The supplier describes the enzyme as a molecular tool for various biochemical applications, highlighting its role in studies of carbohydrate metabolism and sucrose utilization. The concise documentation fields—amount, supplied-as, storage, keywords, and biotechnology-grade note—support clean SOP inclusion and inventory traceability in multi-user labs.

In research workflows, sucrose phosphorylase is often used to generate glucose-1-phosphate in vitro or to demonstrate phosphorolysis in qualitative teaching and training modules. Because the reaction is reversible, teams can explore conditions that favor either phosphorolysis or the reverse direction while documenting buffer composition (pH, ionic strength), Pi and substrate levels, temperature, and incubation time. The lyophilized format enables precise aliquoting and flexible reconstitution into the lab’s preferred buffer system. Since the product page does not prescribe a specific activity assay format or working concentration, a practical approach is to perform small pilot titrations that establish a reproducible operating window for the intended matrix and readout. Recording lot identifiers and time-at-temperature throughout handling helps maintain performance consistency across analysts and instruments.

Typical scenarios include assembling enzyme-panel comparisons for carbohydrate conversion, configuring platform checks where formation of glucose-1-phosphate serves as a qualitative readout, and building instructional demonstrations of reversible glycosyl transfer using sucrose as donor. Within research-only boundaries, the clearly specified fields and storage guidance make this sucrose phosphorylase offering a dependable, documentation-friendly component for laboratories standardizing carbohydrate-metabolism–related workflows and exploratory development work.

Why researchers choose this product:

• Reversible conversion of sucrose + Pi to glucose-1-phosphate and fructose
• Defined 1 kU fill and lyophilized format support precise aliquoting and storage
• Specified storage at −20 C aligns with routine cold-chain handling
• Listed as a molecular tool for diverse biochemical applications
• Documentation-ready fields (amount, MW entry, storage, keywords, grade) for SOP/LIMS mapping

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1: Jiang R, Ye K, Fan T, Lu Y, Zhu L, Chen X, Chen H. [Application of sucrose_phosphorylase in glycosylation] Sheng Wu Gong Cheng Xue Bao. 2021 Jan 25;37(1):112-129.
• 2: Franceus J, Desmet T. Sucrose Phosphorylase and Related Enzymes in Glycoside Hydrolase Family 13: Discovery, Application and Engineering Int J Mol Sci. 2020 Apr 5;21(7):2526.
• 3: Zhou J, Jiang R, Shi Y, Ma W, Liu K, Lu Y, Zhu L, Chen X. Sucrose_phosphorylase from Lactobacillus reuteri: Characterization and application of enzyme for production of 2-O-_-d-glucopyranosyl glycerol Int J Biol Macromol. 2022 Jun 1;209(Pt A):376-384.
• 4: Zhou Y, Ke F, Chen L, Lu Y, Zhu L, Chen X. Enhancing regioselectivity of sucrose phosphorylase by loop engineering for glycosylation of L-ascorbic acid Appl Microbiol Biotechnol. 2022 Jun;106(12):4575-4586.
• 5: Tauzin AS, Bruel L, Laville E, Nicoletti C, Navarro D, Henrissat B, Perrier J, Potocki-Veronese G, Giardina T, Lafond M. Sucrose 6(F)-phosphate phosphorylase_: a novel insight in the human gut microbiome Microb Genom. 2019 Apr;5(4):e000253.
• 6: Zhang H, Zhu L, Zhou Z, Wang D, Yang J, Wang S, Lou T. Advancements in the Heterologous Expression of Sucrose Phosphorylase and Its Molecular Modification for the Synthesis of Glycosylated Products Molecules. 2024 Aug 28;29(17):4086.
• 7: Goux M, Demonceaux M, Hendrickx J, Solleux C, Lormeau E, Fredslund F, Tezé D, Offmann B, André-Miral C. Sucrose phosphorylase from Alteromonas mediterranea: Structural insight into the regioselective _-glucosylation of (+)-catechin Biochimie. 2024 Jun;221:13-19.
• 8: Su R, Zheng W, Li A, Wu H, He Y, Tao H, Zhang W, Zheng H, Zhao Z, Li S. Characterization of a novel sucrose phosphorylase from Paenibacillus elgii and its use in biosynthesis of _-arbutin World J Microbiol Biotechnol. 2023 Dec 7;40(1):24.
• 9: Saburi W, Nihira T, Nakai H, Kitaoka M, Mori H. Discovery of solabiose phosphorylase and its application for enzymatic synthesis of solabiose from sucrose and lactose Sci Rep. 2022 Jan 7;12(1):259.
• 10: Vyas A, Nidetzky B. Energetics of the Glycosyl Transfer Reactions of Sucrose Phosphorylase Biochemistry. 2023 Jun 20;62(12):1953-1963. pubmed.ncbi.nlm.nih.gov