Succinyl-CoA Synthetase (Highly Pure) – Catalog Number: B2012603 (100 ug)

Succinyl-CoA Synthetase (Highly Pure) is listed as a high-quality enzyme that catalyzes the reversible reaction of succinyl-CoA to succinate and is supplied as a lyophilized powder in a defined 100 ug fill (Catalog B2012603) with storage at −20 °C. The product page documents separate entries for the two subunits—29.8 kDa (α) and 41.4 kDa (β)—and designates the reagent as a molecular tool for various biochemical applications. These concise fields support clean SOP inclusion, inventory traceability, and cross-run reproducibility in multi-user labs.

Operationally, succinyl-CoA synthetase (also known as succinate thiokinase) participates in the reversible conversion between succinyl-CoA and succinate. In bench method development, such enzymes are often leveraged to build qualitative assays that monitor conversion under controlled conditions or to provide defined positive controls when validating workflows around metabolism-linked readouts. A lyophilized presentation facilitates precise aliquoting and flexible reconstitution into the lab’s preferred buffer system. Because the listing does not prescribe activity units or working concentrations, good practice is to perform small pilot titrations, record buffer composition (including pH and ionic strength), and document incubation times and temperature history alongside lot identifiers. These records streamline method transfer and help maintain performance consistency across analysts and instruments.

Typical research scenarios include constructing qualitative conversion assays for instructional use, assembling platform checks where a well-characterized enzyme provides a predictable response, and configuring exploratory reaction chains in which succinyl-CoA or succinate availability is gated by an enzymatic step. The clear specification set—amount, supplied-as, storage, molecular-weight entries, and biotechnology-grade note—aligns with expectations for low background during optimization. Within research-only boundaries, this offering provides a dependable, documentation-friendly component for laboratories standardizing workflows that touch the succinyl-CoA ⇌ succinate node.

Why researchers choose this product:

• Listed role: catalyzes the reversible reaction of succinyl-CoA to succinate
• Lyophilized powder format supports precise aliquoting and flexible buffer selection
• Separate α (29.8 kDa) and β (41.4 kDa) entries aid documentation and record-keeping
• Specified storage at −20 °C fits routine cold-chain handling
• Designated as a molecular tool for various biochemical applications

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1: Huang J, Fraser ME. Tartryl-CoA inhibits succinyl-CoA synthetase Acta Crystallogr F Struct Biol Commun. 2020 Jul 1;76(Pt 7):302-308.
• 2: Wu B, Qiu J, Zhao TV, Wang Y, Maeda T, Goronzy IN, Akiyama M, Ohtsuki S, Jin K, Tian L, Goronzy JJ, Weyand CM. Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T Cells Cell Metab. 2020 Dec 1;32(6):967-980.e5.
• 3: Huang J, Fraser ME. The structure of succinyl-CoA synthetase bound to the succinyl-phosphate intermediate clarifies the catalytic mechanism of ATP-citrate lyase Acta Crystallogr F Struct Biol Commun. 2022 Oct 1;78(Pt 10):363-370.
• 4: Nishimura JS. Succinyl-CoA synthetase structure-function relationships and other considerations Adv Enzymol Relat Areas Mol Biol. 1986;58:141-72.
• 5: Tong Y, Guo D, Lin SH, Liang J, Yang D, Ma C, Shao F, Li M, Yu Q, Jiang Y, Li L, Fang J, Yu R, Lu Z. SUCLA2-coupled regulation of GLS succinylation and activity counteracts oxidative stress in tumor cells Mol Cell. 2021 Jun 3;81(11):2303-2316.e8.
• 6: Huang J, Nguyen VH, Hamblin KA, Maytum R, van der Giezen M, Fraser ME. ATP-specificity of succinyl-CoA synthetase from Blastocystis hominis Acta Crystallogr D Struct Biol. 2019 Jul 1;75(Pt 7):647-659.
• 7: Huang J, Fraser ME. Second distinct conformation of the phosphohistidine loop in succinyl-CoA synthetase Acta Crystallogr D Struct Biol. 2021 Mar 1;77(Pt 3):357-368.
• 8: Liu X, Si W, He L, Yang J, Peng Y, Ren J, Liu X, Jin T, Yu H, Zhang Z, Cheng X, Zhang W, Xia L, Huang Y, Wang Y, Liu S, Shan L, Zhang Y, Yang X, Li H, Liang J, Sun L, Shang Y. The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry Signal Transduct Target Ther. 2021 Nov 3;6(1):375.
• 9: Gomes C, Palma N, Pons MJ, Magallón-Tejada A, Sandoval I, Tinco-Valdez C, Gutarra C, Del Valle-Mendoza J, Ruiz J, Matsuoka M. Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella bacilliformis PLoS Negl Trop Dis. 2016 Sep 14;10(9):e0004989.
• 10: Vashisht K, Singh P, Verma S, Dixit R, Mishra N, Pandey KC. The nucleotide specificity of succinyl-CoA synthetase of Plasmodium falciparum is not determined by charged gatekeeper residues alone FEBS Open Bio. 2021 Mar;11(3):578-587. pubmed.ncbi.nlm.nih.gov