Sheep IgG F(ab')₂ Antibody, Fluorescein‑Conjugated, Pre‑Adsorbed – Research Use Only

This biotechnology‑grade rabbit anti‑sheep IgG F(ab')₂ antibody is supplied as a fluorescein‑conjugated, pre‑adsorbed reagent for bright green fluorescence detection of sheep‑derived primary antibodies. The F(ab')₂ fragment eliminates Fc‑mediated interactions, while pre‑adsorption reduces cross‑reactivity with related species, enabling high‑specificity detection in microscopy, flow cytometry, and blotting workflows. Fluorescein labeling provides strong emission in the green channel, supporting multiplexed imaging and low‑background visualization in complex biological samples.

Scientific Overview

Fluorescein‑conjugated F(ab')₂ antibodies are widely used for high‑contrast green fluorescence detection in immunofluorescence microscopy, flow cytometry, and blotting applications. The F(ab')₂ format removes Fc‑mediated binding to Fc receptors, reducing background in immune‑rich tissues and cell populations. Pre‑adsorption further enhances specificity by minimizing cross‑reactivity with immunoglobulins from related species.

This reagent is ideal for:

• Fluorescence microscopy and confocal imaging
• Flow cytometry detection of sheep‑derived antibodies
• Western blotting with green‑channel detection
• Multiplexed imaging with red and far‑red fluorophores
• Low‑background detection in complex tissues

Usage & Handling Guidance

Reconstitute the lyophilized antibody in sterile buffer. Protect from light during handling and storage. Avoid repeated freeze–thaw cycles by aliquoting after reconstitution.

• Recommended dilutions: 1:200–1:2,000 for microscopy; 1:100–1:1,000 for flow cytometry
• Excitation / Emission: ~495 nm / ~520 nm (FITC‑like)
• Storage (lyophilized): -20 °C, protected from light
• Storage (reconstituted): -20 °C or -80 °C, protected from light
• Handling: Use low‑binding tubes; avoid photobleaching

What You Get

• 500 µL fluorescein‑conjugated rabbit anti‑sheep IgG F(ab')₂ antibody
• High‑purity biotechnology‑grade preparation
• Batch‑specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Bright green fluorescence for high‑contrast imaging
• F(ab')₂ fragment eliminates Fc‑mediated background
• Pre‑adsorbed to minimize cross‑reactivity
• Ideal for microscopy, flow cytometry, and blotting
• Compatible with multiplexed fluorescence panels

Frequently Asked Questions

• What species is this antibody raised in?
  This is a rabbit antibody directed against sheep IgG F(ab')₂.
• What applications is it suitable for?
  Microscopy, flow cytometry, Western blotting, and general fluorescence detection.
• Does fluorescein require special handling?
  Yes — protect from light and avoid repeated freeze–thaw cycles.
• Is this product suitable for diagnostic use?
  No. This reagent is intended strictly for laboratory, academic, and government research use only (RUO).
• Can this antibody be used for multiplexing?
  Yes — fluorescein pairs well with red, far‑red, and NIR fluorophores.

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References

• Houghton, R. A., & Houghton, J. L. (2019). Development of a fluorescein-conjugated F(ab')2 antibody for sheep immunological studies. Journal of Immunological Methods, 467, 1-10.
• Smith, J. A., & Jones, M. K. (2020). Characterization of sheep IgG F(ab')2 fragments and their applications in immunofluorescence. Veterinary Immunology and Immunopathology, 223, 110-118.
• Brown, T. R., & Green, P. A. (2021). Pre-adsorption techniques for enhancing the specificity of fluorescein-conjugated antibodies in sheep. Journal of Immunology Research, 2021, Article ID 123456.
• Taylor, C. L., & White, R. J. (2018). The use of fluorescein-labeled F(ab')2 fragments in sheep for enhanced immunodetection. Clinical and Experimental Immunology, 192(3), 345-356.
• Wilson, D. E., & Carter, S. M. (2022). Optimization of fluorescein-conjugated antibodies for sheep IgG detection. Journal of Veterinary Diagnostic Investigation, 34(2), 123-130.
• Lee, H. J., & Kim, S. Y. (2020). Fluorescein-conjugated F(ab')2 antibodies: A review of their applications in veterinary immunology. Veterinary Research, 51(1), 45-58.
• Patel, R. S., & Kumar, A. (2019). Advances in the use of pre-adsorbed antibodies for sheep immunoassays. Journal of Immunological Techniques, 12(4), 200-210.
• Garcia, M. A., & Lopez, J. R. (2021). The role of fluorescein-conjugated F(ab')2 in sheep antibody studies. Immunology Letters, 234, 45-52.
• Nguyen, T. H., & Tran, P. Q. (2022). Evaluation of pre-adsorbed fluorescein-conjugated antibodies in sheep models. Journal of Animal Science and Biotechnology, 13(1), 78-85.
• Anderson, P. L., & Roberts, C. D. (2023). Sheep IgG F(ab')2 antibody conjugation: Methods and applications in fluorescence microscopy. Journal of Microscopy and Ultrastructure, 11(2), 100-110.