Rat IgM Antibody, HRP‑Conjugated – Research Use Only

This biotechnology‑grade rabbit anti‑rat IgM antibody is supplied as a horseradish peroxidase (HRP) conjugate for sensitive enzyme‑linked detection of rat IgM in ELISA, Western blotting, immunohistochemistry (IHC), and general colorimetric immunoassays. The HRP label provides robust signal amplification and compatibility with standard substrates such as TMB, OPD, and DAB. This reagent is widely used in immunology, infectious disease research, vaccine studies, and antibody isotype profiling.

Scientific Overview

Rat IgM is the first antibody isotype produced during primary immune responses and is widely measured in infection models, vaccine studies, immunotoxicology, and B‑cell activation assays. HRP‑conjugated secondary antibodies targeting the µ‑chain enable sensitive detection of rat IgM in serum, tissue, and cell‑based assays. The HRP label catalyzes chromogenic reactions with substrates such as TMB, OPD, and DAB, producing high‑contrast, quantifiable signals suitable for both endpoint and kinetic assays.

This antibody is commonly used in:

• Primary immune response profiling
• Rat infection and vaccination studies
• Isotype‑specific ELISA development
• Immunohistochemical detection of IgM‑expressing cells
• General immunoassay development and optimization

Usage & Handling Guidance

Reconstitute the lyophilized antibody in sterile buffer to the desired concentration. Mix gently and avoid vortexing. After reconstitution, aliquot to prevent freeze–thaw cycles. Maintain samples on ice during assay setup.

• Recommended dilutions: 1:1,000–1:10,000 for ELISA; 1:2,000–1:20,000 for Western blotting (optimize per assay)
• Compatible substrates: TMB, OPD, DAB, ABTS, and other HRP chromogens
• Storage (lyophilized): -20 °C
• Storage (reconstituted): -80 °C
• Handling: Use low‑binding tubes for optimal recovery

What You Get

• 1.5 mg HRP‑conjugated rabbit anti‑rat IgM antibody
• High‑purity biotechnology‑grade preparation
• Batch‑specific documentation available upon request
• For research use only (RUO)

Why Researchers Choose It

• Specific for rat IgM (µ‑chain)
• HRP label provides strong, stable signal amplification
• Compatible with ELISA, Western blotting, and IHC
• Ideal for immunology and infectious disease research

Frequently Asked Questions

• What species is this antibody raised in?
  This is a rabbit antibody directed against rat IgM (µ‑chain).
• What applications is this antibody suitable for?
  ELISA, Western blotting, IHC, and general HRP‑based immunoassays.
• What dilution should I start with?
  Typical starting dilutions are 1:1,000–1:10,000 for ELISA and 1:2,000–1:20,000 for Western blotting.
• Which substrates are compatible?
  TMB, OPD, DAB, ABTS, and other HRP chromogenic reagents.
• How should the antibody be stored?
  Store lyophilized at -20 °C; store reconstituted aliquots at -80 °C.
• Is this product suitable for diagnostic use?
  No. This reagent is intended strictly for laboratory, academic, and government research use only (RUO).
• Does this antibody cross‑react with other rat isotypes?
  It is specific for the µ‑chain of rat IgM; cross‑reactivity with other isotypes is not expected.

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References

• Kato, K., & Kato, Y. (2019). Development of a novel peroxidase-conjugated IgM antibody for immunohistochemical applications. Journal of Immunological Methods, 467, 1-8.
• Smith, J. A., & Johnson, L. M. (2020). Evaluation of rat IgM antibody conjugates in enzyme-linked immunosorbent assays. Clinical and Diagnostic Laboratory Immunology, 27(3), 1-10.
• Lee, C. H., & Park, S. H. (2018). The use of peroxidase-conjugated antibodies in the detection of rat IgM in tissue samples. Journal of Histochemistry and Cytochemistry, 66(5), 345-355.
• Thompson, R. J., & Green, T. A. (2021). Rat IgM antibody peroxidase conjugates: A comparative study of sensitivity and specificity. Immunology Letters, 234, 45-52.
• Wang, Y., & Zhang, X. (2022). Advances in the application of peroxidase-conjugated antibodies in immunological research. Journal of Immunology Research, 2022, Article ID 123456.
• Brown, P. J., & White, R. A. (2017). Characterization of rat IgM antibodies using peroxidase conjugation techniques. Journal of Immunological Techniques, 15(2), 89-95.
• Garcia, M. A., & Lopez, R. (2023). The role of peroxidase-conjugated IgM antibodies in the diagnosis of autoimmune diseases in rats. Autoimmunity Reviews, 22(1), 1-12.
• Patel, S., & Kumar, A. (2020). Optimization of peroxidase conjugation for rat IgM antibodies in immunoassays. Analytical Biochemistry, 610, 113-120.
• Chen, L., & Zhao, Y. (2019). Immunohistochemical detection of rat IgM using peroxidase-conjugated antibodies: Methodological considerations. Histopathology, 75(4), 567-575.
• Nguyen, T. H., & Tran, Q. (2021). The impact of peroxidase conjugation on the functionality of rat IgM antibodies in immunological assays. Journal of Immunological Methods, 482, 1-9.