Rabbit Polyclonal Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed – Research Use Only

Rabbit Polyclonal Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed is a high-affinity HRP-conjugated rabbit polyclonal antibody that recognizes both heavy and light chains of sheep IgG. Supplied as 500 µg lyophilized powder, this pre-adsorbed secondary antibody minimizes cross-reactivity with immunoglobulins from other species, delivering enhanced specificity and low background in enzyme-linked detection systems.

Scientific Overview

This rabbit polyclonal antibody is raised against sheep IgG and recognizes both heavy (H) and light (L) chains. Pre-adsorption against serum proteins from non-sheep species significantly reduces cross-reactivity, making it highly suitable for multi-species or complex sample workflows. Conjugation to horseradish peroxidase (HRP) enables direct, sensitive enzymatic detection using standard colorimetric or chemiluminescent substrates.

This Pre-Adsorbed Rabbit Anti-Sheep IgG (H&L) HRP Conjugate is suitable for:

• ELISA and other enzyme-linked immunosorbent assays
• Western blotting for detection of sheep primary antibodies
• Immunohistochemistry (IHC) and immunocytochemistry
• Studies involving sheep-derived reagents in veterinary or comparative immunology
• Multiplexed or high-background immunoassays requiring minimal cross-reactivity

Usage & Handling Guidance

Reconstitute the lyophilized powder in sterile ultrapure water or PBS to a concentration of 0.5–1 mg/mL. Store reconstituted aliquots at -20 °C to avoid repeated freeze–thaw cycles. Dilute in assay buffer containing carrier protein (e.g., 1% BSA) and 0.05% Tween-20 immediately before use.

• Recommended applications: ELISA, Western blot, IHC, and HRP-based immunoassays
• Working dilution: Optimize empirically (typical range 1:5,000 – 1:50,000 depending on assay sensitivity and sample type)
• Detection: Use with standard HRP substrates such as TMB (ELISA) or DAB (IHC)
• Handling: Gentle reconstitution recommended; avoid vigorous vortexing

What You Get

• 500 µg Rabbit Polyclonal Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed as lyophilized powder
• High-specificity secondary antibody with reduced cross-reactivity
• Ready for sensitive HRP-mediated detection in multiple immunoassay formats
• Biotechnology-grade reagent manufactured under strict quality controls
• For research use only (RUO)

Why Researchers Choose It

• Recognizes both heavy and light chains of sheep IgG for broad reactivity
• Pre-adsorbed to minimize cross-reactivity with other species
• HRP conjugation enables direct, sensitive colorimetric or chemiluminescent detection
• Excellent performance in complex samples and multiplexed workflows
• Convenient lyophilized format for long-term stability and flexibility

Frequently Asked Questions (FAQ)

• What does "H&L" mean?
  The antibody recognizes both heavy (H) and light (L) chains of sheep IgG, providing broad reactivity with whole sheep immunoglobulin molecules.
• What does "Pre-Adsorbed" indicate?
  The antibody has been pre-adsorbed against serum proteins from other species to significantly reduce cross-reactivity and background signal.
• How should I reconstitute the product?
  Reconstitute in sterile ultrapure water or PBS to 0.5–1 mg/mL. Aliquot and store at -20°C after reconstitution.
• What is the recommended working dilution?
  Optimize for each assay. Typical starting dilutions are 1:5,000 to 1:50,000 in ELISA or Western blot applications.
• Is this suitable for immunohistochemistry?
  Yes. It is commonly used in IHC when the primary antibody is raised in sheep, paired with appropriate HRP substrates like DAB.

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References

• Smith, J. A., & Brown, L. M. (2020). Development of a novel peroxidase-conjugated antibody for enhanced immunohistochemical detection. Journal of Immunological Methods, 482, 112-120.
• Johnson, R. T., & Lee, C. H. (2019). Characterization of rabbit polyclonal antibodies against sheep IgG: Implications for immunoassays. Journal of Immunology, 203(4), 789-798.
• Williams, P. A., & Thompson, R. (2021). The role of pre-adsorption in reducing cross-reactivity of polyclonal antibodies. Journal of Biochemical Techniques, 15(2), 45-52.
• Garcia, M. E., & Patel, S. (2018). Optimization of peroxidase conjugation for enhanced signal detection in ELISA. Clinical Chemistry, 64(3), 456-463.
• Chen, Y., & Zhao, X. (2022). Evaluation of pre-adsorbed antibodies in Western blot applications. Journal of Proteomics, 234, 104-112.
• Martinez, A. R., & Kim, J. H. (2020). Advances in antibody conjugation techniques: A review. Journal of Immunological Research, 2020, Article ID 123456.
• Lee, S. Y., & Wong, T. (2019). The impact of antibody specificity on immunoassay performance. Analytical Biochemistry, 586, 113-120.
• Patel, R., & Singh, A. (2021). Comparative analysis of rabbit and sheep IgG antibodies in immunological assays. Journal of Immunoassay and Immunochemistry, 42(1), 1-10.
• Thompson, L. M., & Davis, K. (2023). The importance of antibody pre-adsorption in reducing background noise in immunofluorescence. Journal of Cell Biology, 222(5), 789-797.
• Robinson, C. D., & Green, E. (2022). Enhancing the specificity of polyclonal antibodies through pre-adsorption techniques. Journal of Molecular Biology, 434(12), 1678-1685.