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Phosphorylase-b Enzyme Proteins Molecular Depot
Phosphorylase-b Enzyme Proteins Molecular Depot
Phosphorylase-b Enzyme Proteins Molecular Depot
Phosphorylase-b Enzyme Proteins Molecular Depot

Phosphorylase-b Enzyme

$647.00

    Catalog Number: B2013577 (1 mg)

    Phosphorylase-b Enzyme is a high quality Phosphorylase-b enzyme. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Phosphorylase-b Enzyme – Catalog Number: B2013577 (1 mg)

Catalog number: B2013577
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 1 mg
Molecular Weight or Concentration: 60 U/mg protein
Supplied as: Powder
Applications: a molecular tool for various biochemical applications
Storage: -20 °C
Keywords: Phosphorylase-b
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

Scientific Description

Phosphorylase-b Enzyme (Catalog B2013577, 1 mg powder) is a muscle-specific form of glycogen phosphorylase provided at an activity of approximately 60 U/mg protein. This enzyme catalyzes the phosphorolysis of glycogen to release glucose-1-phosphate in the presence of inorganic phosphate, playing a pivotal role in biochemical studies of glycogen metabolism and enzyme regulation. Packaged in a lyophilized powder and stored at –20 °C, this biotechnology-grade reagent supports long-term stability and precise experimental reproducibility, especially in workflows with cold-chain requirements.

In investigative and assay development contexts, Phosphorylase-b Enzyme is invaluable for exploring allosteric regulation, phosphorylation cascades, and energy-dependent enzymatic control within metabolic networks. Its consistent activity profile enables researchers to calibrate glycogenolytic rates, validate phosphatase/kinase system responses, and construct kinetic models. The powdered format facilitates exact aliquoting and protocol fidelity across multi-user environments.

Typical applications include activity assays with glycogen substrates, characterizing phosphorylase-a conversion via kinase pathways, and serving as an analytical standard in high-throughput screening systems. The defined activity metric, institutional compatibility, and compliance with SOP and LIMS workflows make this enzyme a reliable asset for enzymology, metabolic regulation, and educational modules in graduate-level biochemistry and physiology.

Why researchers choose this product:

  • Defined 60 U/mg activity supports precision quantification in glycogenolysis studies
  • Lyophilized powder ensures stable storage and easy aliquoting
  • Biotechnology-grade purity reduces variability and supports sensitive detection assays
  • Suitable for enzyme regulation assays, phospho-conversion models, and kinetic profiling

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

  • Van den Berg IE, Berger R. Phosphorylase b kinase deficiency in man: a review J Inherit Metab Dis. 1990;13(4):442-51.
  • Clemens PR, Yamamoto M, Engel AG. Adult phosphorylase b kinase deficiency Ann Neurol. 1990 Oct;28(4):529-38.
  • Livanova NB, Chebotareva NA, Eronina TB, Kurganov BI. Pyridoxal 5′-phosphate as a catalytic and conformational cofactor of muscle glycogen phosphorylase B Biochemistry (Mosc). 2002 Oct;67(10):1089-98.
  • Jenkins JA, Johnson LN, Stuart DI, Stura EA, Wilson KS, Zanotti G. Phosphorylase: control and activity Philos Trans R Soc Lond B Biol Sci. 1981 Jun 26;293(1063):23-41.
  • Morange M, Buc H. The interplay between covalent and non-covalent regulation of glycogen phosphorylase. The role of different effectors of phosphorylase b on the phosphorylase b to a conversion rate Biochimie. 1979;61(5-6):633-43.
  • Marquetant R, Manfredi JP, Holmes EW. Binding of phosphorylase a and b to skeletal muscle thin filament proteins Arch Biochem Biophys. 1986 Mar;245(2):404-10.
  • Stalmans W, Hers HG. The stimulation of liver phosphorylase b by AMP, fluoride and sulfate. A technical note on the specific determination of the a and b forms of liver glycogen phosphorylase Eur J Biochem. 1975 Jun;54(2):341-50.
  • Naranan V, Brickey DA, Rutherford CL. Glycogen phosphorylase ‘b’ in Dictyostelium: stability and endogenous phosphorylation Mol Cell Biochem. 1988 Sep;83(1):89-104.
  • Barron JT, Kopp SJ. Phosphorylase b kinase and phosphorylase a phosphatase activities in contracting vascular smooth muscle: stimulation by fatty acid Biochim Biophys Acta. 1991 Apr 9;1073(3):550-4.
  • Eronina TB, Mikhaylova VV, Chebotareva NA, Kleymenov SY, Pivovarova AV, Kurganov BI. Combined action of chemical chaperones on stability, aggregation and oligomeric state of muscle glycogen phosphorylase b Int J Biol Macromol. 2022 Apr 1;203:406-416.

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

    Purchase Orders
    Confirmed orders are final and may not be canceled or refunded unless authorized in writing.

    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

    Safety Guidelines
    Always consult the product’s SDS. Products must not be ingested, inhaled, or come into contact with skin or eyes unless specified.

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    All changes must be in writing and approved. Cancellations may result in fees for materials, labor, or third-party costs.

    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

    Delivery & Claims
    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

    Extended Payment Terms
    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
    Ownership remains with Blue Tiger Scientific or Molecular Depot LLC until payment is complete. Goods must be stored separately and labeled until paid in full.

    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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