Normal Goat Serum (10%) – Catalog #B2010768

Normal Goat Serum (10%) (Catalog #B2010768) is a convenient, ready-to-use 10% solution of high-quality goat serum supplied in a 50 mL volume. It is an effective blocking agent and antibody diluent for immunohistochemistry (IHC), immunofluorescence (IF), Western blotting, ELISA, and other immunoassays. The pre-diluted 10% formulation simplifies protocol setup while reducing non-specific binding and background noise in antibody-based detection systems.

Scientific Overview

Normal Goat Serum (10%) is a pre-diluted blocking reagent derived from healthy, non-immunized goats. It contains a broad spectrum of proteins that effectively saturate non-specific binding sites on tissues, membranes, and assay surfaces. This ready-to-use 10% formulation is ideal for minimizing background staining and improving signal specificity in antibody-based assays, including those using goat-derived secondary antibodies.

Key applications include:

• Blocking in immunohistochemistry (IHC) and immunofluorescence (IF)
• Antibody diluent for primary and secondary antibodies
• Reduction of non-specific binding in Western blotting and ELISA
• General serum control in immunological protocols

Usage & Handling Guidance

Store at -20°C. Thaw at 2–8°C or room temperature before use. Typical final concentration for blocking: 5–10% (already provided at 10%). For antibody dilution, use 1–5% in PBS-T. Heat-inactivate (56°C for 30 min) if complement activity is a concern.

• Blocking step: Incubate with 10% normal goat serum for 30–60 min
• Diluent: Dilute antibodies in 1–5% normal goat serum
• Aliquoting: Recommended to avoid repeated freeze–thaw cycles

What You Get

• 50 mL Normal Goat Serum (10% ready-to-use solution)
• Convenient pre-diluted format for immediate use in blocking and dilution steps
• High-quality goat serum for low background in immunoassays
• For research use only (RUO)

Why Researchers Choose It

• Ready-to-use 10% solution saves preparation time
• Effective reduction of non-specific binding and background
• Compatible with most primary antibodies and assay formats
• Consistent quality suitable for publication-grade results
• Standard blocking reagent used in thousands of immunological protocols

Frequently Asked Questions (FAQ)

• What is the advantage of the 10% pre-diluted format?
  It is ready-to-use for blocking and dilution, reducing preparation steps and variability.
• Should I heat-inactivate the serum?
  Yes, if complement-mediated interference is a concern in your assay (56°C for 30 min).
• What concentration should I use for blocking?
  The 10% solution can be used directly or further diluted to 5% depending on the protocol.
• How should I store it?
  Store at -20°C. Thaw gently and aliquot to avoid repeated freeze–thaw cycles.
• Is it suitable when using goat secondary antibodies?
  Yes. Normal goat serum is commonly used as a blocker even when goat secondaries are employed.

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References

• Nanda PK, Swain P, Nayak SK, Dash S, Routray P, Swain SK, Patra BC. Goat serum as an alternative to establish cell culture from Indian major carp, Cirrhinus mrigala. In Vitro Cell Dev Biol Anim. 2009 Mar-Apr;45(3-4):148-51.
• Sutton I, Kermode AG. Hyperimmune goat serum (Aimspro): hope or hype? J Clin Neurosci. 2006 Dec;13(10):1037-8.
• Gunn A. Antilymphocytic serum. Br J Haematol. 1969 Nov;17(5):421-3.
• Attia MM, Saad MF, Abdel-Salam AB. Milk as a substitute for serum in diagnosis of toxoplasmosis in goats. J Egypt Soc Parasitol. 2017 Apr;47(1):227-234.
• Dalman A, Eftekhari-Yazdi P, Valojerdi MR, Shahverdi A, Gourabi H, Janzamin E, Fakheri R, Sadeghian F, Hasani F. Synchronizing cell cycle of goat fibroblasts by serum starvation causes apoptosis. Reprod Domest Anim. 2010 Oct;45(5):e46-53.
• Tibbo M, Jibril Y, Woldemeskel M, Dawo F, Aragaw K, Rege JE. Serum enzyme levels and influencing factors in three indigenous Ethiopian goat breeds. Trop Anim Health Prod. 2008 Dec;40(8):657-66.
• Glasscock JL, Whitney TR, Navarro JR, Angle SG, Holmes AR, Stewart WC, Scholljegerdes EJ. Substituting ground woody plants for cottonseed hulls in kid goat feedlot diets: growth performance and blood serum chemistry. J Anim Sci. 2018 Jun 29;96(7):2851-2860.
• Adu FD, Joannis T. Goat hyperimmune serum for the diagnosis of peste des petits ruminants. Vet Rec. 1985 Nov 2;117(18):472.
• Al-Zadjali A, Johnson EH, Srikandakumar A. Serum vitamin B12 levels in Omani goats. Trop Anim Health Prod. 2004 Jul;36(5):473-82.