Normal Goat Serum – Catalog #B2023546

Normal Goat Serum (Catalog #B2023546) is a high-quality 50 mL serum from healthy goats, supplied ready for use in immunological applications. It serves as an essential blocking agent and diluent in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting, and other immunoassays. Normal goat serum effectively reduces non-specific binding of primary and secondary antibodies, improving signal-to-noise ratio and assay specificity across human, mouse, and rat systems.

Scientific Overview

Normal Goat Serum is a pooled serum collected from healthy, non-immunized goats. It contains a complex mixture of proteins, including immunoglobulins and albumins, that effectively block non-specific binding sites on tissues, membranes, and plastic surfaces. It is a standard blocking reagent in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting, ELISA, and other antibody-based assays, significantly reducing background noise and improving signal specificity in human, mouse, and rat systems.

Primary uses include:

• Blocking non-specific antibody binding in IHC and IF
• Diluent for primary and secondary antibodies
• Reducing background in Western blots and ELISAs
• General serum control in immunological protocols

Usage & Handling Guidance

Store at -20°C. Thaw at 2–8°C or room temperature. Heat-inactivate (56°C for 30 min) if complement activity must be removed. Typical working concentration: 5–10% (v/v) in blocking buffer or antibody diluent. Filter through 0.22 µm if sterility is required.

• Recommended blocking: 5–10% normal goat serum in PBS-T for 30–60 min
• Diluent: 1–5% normal goat serum in PBS-T for antibody incubation
• Aliquoting: Prepare single-use aliquots to avoid repeated freeze–thaw cycles

What You Get

• 50 mL Normal Goat Serum
• High-quality, pooled serum from healthy goats
• Excellent blocking reagent for multiple immunoassay platforms
• Convenient volume for routine laboratory use
• For research use only (RUO)

Why Researchers Choose It

• Proven performance as a blocking agent in IHC, IF, and Western blotting
• Reduces non-specific binding and background noise effectively
• Compatible with most mammalian primary and secondary antibodies
• Stable, ready-to-use serum with consistent quality
• Standard reagent used in thousands of published immunological protocols

Frequently Asked Questions (FAQ)

• What is the primary use of Normal Goat Serum?
  It is primarily used as a blocking agent and antibody diluent to minimize non-specific binding in immunoassays.
• Should I heat-inactivate the serum?
  Yes, heat inactivation (56°C for 30 min) is recommended if complement activity could interfere with your assay.
• What concentration should I use for blocking?
  Typically 5–10% (v/v) in PBS-T for 30–60 minutes at room temperature.
• How should I store the serum?
  Store at -20°C. Thaw at 2–8°C or room temperature and prepare aliquots to avoid repeated freeze–thaw cycles.
• Is this serum suitable for all species?
  It is most commonly used with human, mouse, and rat samples; test compatibility for other species.

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References

• Harlow E, Lane D. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press; 1988.
• Hsu SM, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981;29(4):577-580.
• Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem. 1970;18(5):315-333.
• Coons AH, Creech HJ, Jones RN, Berliner E. The demonstration of pneumococcal antigen in tissues by the use of fluorescent antibody. J Immunol. 1942;45:159-170.
• Kawai T, Akira S. Antiviral signaling through pattern recognition receptors. J Biochem. 2007 Feb;141(2):137-145.
• Buchwalow I, Samoilova V, Boecker W, Tiemann M. Non-specific binding of antibodies in immunohistochemistry: fallacies and facts. Sci Rep. 2011;1:28.
• Kim SW, Roh J, Park CS. Immunohistochemistry for pathologists: protocols, pitfalls, and tips. J Pathol Transl Med. 2016 Nov;50(6):411-418.
• Coons AH, Creech HJ, Jones RN. Immunological properties of an antibody containing a fluorescent group. Proc Soc Exp Biol Med. 1941;47:200-202.
• Hsu SM, Raine L. Protein A, avidin, and biotin in immunohistochemistry. J Histochem Cytochem. 1981;29(11):1349-1353.
• Hsu SM, Raine L, Fanger H. The use of antiavidin antibody and avidin-biotin-peroxidase complex in immunoperoxidase technics. Am J Clin Pathol. 1981;75(6):816-821.