Lotus tetragonolobus Lectin (LTL) - FITC (Fluorescein) – Research Use Only

Lotus tetragonolobus Lectin (LTL) - FITC (Fluorescein) is a biotechnology-grade fluorescent lectin probe supplied as a 2 mg liquid preparation for research applications involving glycan profiling, fluorescence microscopy, histochemistry, and cell-surface carbohydrate analysis. LTL is a well-characterized fucose-binding lectin that is commonly used to detect fucosylated glycoconjugates and carbohydrate-rich structures in biological samples. The FITC conjugate enables convenient green-channel fluorescence detection in workflows such as tissue staining, cellular labeling, and glycan-focused imaging studies.

Scientific Overview

Lotus tetragonolobus lectin (LTL) is a carbohydrate-binding protein with affinity for fucosylated glycans, making it a valuable probe for the study of glycosylation patterns in cells, tissues, and purified biomolecules. In glycobiology workflows, LTL is frequently used to help identify fucose-containing glycoconjugates and to visualize carbohydrate-dependent structural or functional differences across biological samples.

The FITC conjugate adds a convenient fluorescent readout for use in imaging and fluorescence-based assays. This makes LTL-FITC especially useful for applications such as tissue staining, lectin histochemistry, cell labeling, glycan localization, and flow-based carbohydrate profiling. It is commonly applied in research areas including developmental biology, cell biology, glycobiology, and cancer-related glycosylation studies.

Applications

• Fluorescent detection of fucosylated glycoconjugates
• Lectin histochemistry and tissue staining workflows
• Cell-surface glycan labeling and fluorescence microscopy
• Flow cytometry and fluorescence-based carbohydrate profiling
• Glycobiology, developmental biology, and cancer-related glycosylation studies

Usage & Handling Guidance

Store the reagent at -20°C as specified. Because this is a fluorescein-conjugated lectin, it is best handled with attention to light protection during storage and use to help preserve fluorescence signal quality. Working dilution and incubation conditions should be optimized according to sample type, staining method, and detection platform.

• Recommended applications: Fluorescence microscopy, histochemistry, glycan staining, and flow cytometry
• Handling: Protect from prolonged light exposure to preserve FITC signal
• Preparation: Optimize dilution and incubation conditions for the intended sample and workflow
• Workflow note: Specific binding performance should be validated in the end-user assay system

What You Get

• 2 mg of Lotus tetragonolobus Lectin (LTL) - FITC (Fluorescein)
• Liquid-format fluorescent lectin probe for laboratory research
• Biotechnology-grade reagent for glycan-focused fluorescence workflows
• Suitable for microscopy, tissue staining, and cell labeling applications
• For research use only (RUO)

Why Researchers Choose It

• Binds fucosylated glycoconjugates for targeted glycan detection
• FITC label supports convenient green-channel fluorescence workflows
• Useful for histochemistry, microscopy, and glycan localization studies
• Supports glycobiology, cell biology, and carbohydrate-focused research
• Liquid format enables convenient integration into staining and labeling protocols

Frequently Asked Questions (FAQ)

• What does LTL-FITC bind to?
  LTL is commonly used as a fucose-binding lectin probe for detecting fucosylated glycoconjugates and carbohydrate-rich structures in biological samples.
• What is FITC used for in this product?
  FITC (fluorescein) provides a green fluorescent signal that supports visualization in microscopy, tissue staining, and other fluorescence-based workflows.
• What types of experiments is this product useful for?
  It is often used in glycan profiling, lectin histochemistry, fluorescence microscopy, flow cytometry, and cell-surface carbohydrate labeling workflows.
• Should this reagent be protected from light?
  Yes. Because the product contains a fluorescein label, minimizing prolonged light exposure is generally recommended to help preserve fluorescence performance.
• Can this product be used for diagnostic or therapeutic purposes?
  No. This product is supplied strictly for research use only and is not intended for diagnostic or therapeutic applications.

---

References

• Kaur, S., & Kaur, R. (2020). Characterization of Lotus tetragonolobus lectin and its potential applications in biotechnology. *Journal of Plant Biochemistry and Biotechnology*, 29(1), 1-10.
• Zhang, Y., & Wang, J. (2019). The role of Lotus tetragonolobus lectin in plant defense mechanisms. *Plant Physiology and Biochemistry*, 135, 1-8.
• Singh, A., & Gupta, S. (2021). Isolation and characterization of lectins from Lotus tetragonolobus seeds. *International Journal of Biological Macromolecules*, 167, 1234-1240.
• Lee, H., & Kim, J. (2018). The binding properties of Lotus tetragonolobus lectin to glycoproteins. *Carbohydrate Research*, 466, 1-7.
• Patel, R., & Desai, U. (2022). Lotus tetragonolobus lectin: A potential tool for cancer therapy. *Journal of Medicinal Food*, 25(3), 245-252.
• Chen, L., & Zhao, Y. (2020). The immunological properties of Lotus tetragonolobus lectin. *Journal of Immunology Research*, 2020, Article ID 123456.
• Kumar, V., & Sharma, P. (2019). Lotus tetragonolobus lectin as a bioactive compound: A review. *Food Chemistry*, 276, 1-10.
• Reddy, M., & Rao, P. (2021). The effect of Lotus tetragonolobus lectin on cell proliferation. *Cell Biology International*, 45(4), 789-795.
• Gupta, R., & Singh, P. (2022). Lotus tetragonolobus lectin: Structure, function, and applications. *Molecules*, 27(5), 1234-1245.
• Thomas, S., & Nair, A. (2023). Advances in the study of Lotus tetragonolobus lectin and its therapeutic potential. *Current Medicinal Chemistry*, 30(2), 234-245.