Glutamine Synthetase – Catalog Number: B2020189 (1 kU)

Glutamine Synthetase (GS) is an essential enzyme in nitrogen metabolism, catalyzing the ATP-dependent synthesis of glutamine from glutamate and ammonia. This listing provides a defined 1 kU enzyme fill (Catalog B2020189) as a lyophilized preparation with a high molecular weight of 900 kDa, stored at −20 °C for long-term stability. Molecular Depot describes it as a molecular tool for various biochemical applications, and its precise documentation (catalog, amount, format, storage, keywords, grade) supports seamless SOP deployment and inventory tracking in collaborative laboratory environments.

Glutamine synthetase plays a dual role—detoxifying ammonia in tissues with high turnover and supplying glutamine for biosynthesis. Its oligomeric, high-mass structure makes it ideal for studying regulatory mechanisms, including allosteric effectors, post-translational modifications, and structural stability. Researchers leverage GS for enzymology assays, metabolic network modeling, and high-throughput screening of modulators or inhibitors.

To integrate effectively into workflows, researchers often reconstitute the lyophilized enzyme under cold conditions using consistent buffer volumes, then calibrate activity using pilot studies that vary substrate levels, pH, and effector concentrations. GS is frequently deployed in cellular studies of nitrogen balance, neurobiology research on ammonia clearance, and in biochemical assays assessing enzyme kinetics. Its documented attributes and purity standard enable reproducibility across analysts, platforms, and timepoints, making it a robust choice for high-fidelity nitrogen metabolism experimentation.

Why researchers choose this product:

• High-unit specification (1 kU) supports quantitative enzyme activity assays
• Oligomeric 900 kDa structure reflective of native GS for regulatory and structural studies
• Lyophilized format allows precise aliquoting and long-term preservation
• −20 °C storage supports cold-chain workflows for consistency
• Biotechnology-grade purity ensures minimal contaminants for sensitive assays

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

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