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Epigen (Human, Recombinant, Highly Pure) Proteins Molecular Depot
Epigen (Human, Recombinant, Highly Pure) Proteins Molecular Depot

Epigen (Human, Recombinant, Highly Pure)

$387.00

    Catalog Number: B2010277 (10 ug)

    Epigen (Human, Recombinant) is a high quality research product used as highly pure Epigen (Human, Recombinant) expressed in CHO cells. This protein acts as an EGF-like growth factor with mitogenic effects on cells expressing ErbB-1. Custom bulk orders of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Epigen (Human, Recombinant, Highly Pure) – Catalog Number: B2010277 (10 ug)

Catalog number: B2010277
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 10 ug
pH: 7.4 upon reconstitution
Supplied as: Lyophilized Powder
Appearance: Powder
Applications: highly pure Epigen (Human, Recombinant) expressed in CHO cells. This protein acts as an EGF-like growth factor with mitogenic effects on cells expressing ErbB-1.
Storage: -20C
Keywords: rHuEpigen; Epithelial mitogen; EPG
Grade: Biotechnology grade. All components are highly pure (minimum 95%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

Epigen (Human, Recombinant) is provided as a lyophilized powder with a defined 10 ug fill (Catalog B2010277) and a listed pH of 7.4 upon reconstitution. The supplier describes this CHO-expressed protein as an EGF-like growth factor with mitogenic effects on cells expressing ErbB-1, aligning it with research that explores epidermal growth factor receptor signaling. The concise specification set—amount, supplied form, reconstitution pH, storage, keywords, and biotechnology-grade note—supports clean SOP inclusion, LIMS mapping, and inventory traceability in multi-user labs.

Within research workflows, recombinant epigen can be incorporated into qualitative studies that examine receptor-dependent responses or pathway activation in cell systems known to express ErbB-1. Because the listing does not prescribe a working concentration or buffer additives beyond reconstitution to pH 7.4, a practical approach is to titrate the protein in small pilot experiments tailored to the matrix and readout being used, such as general proliferation measurements or downstream marker assessments. Documenting buffer composition, incubation time and temperature, and time-at-temperature during handling helps establish a reproducible operating window and simplifies method transfer between analysts and instruments. The lyophilized format facilitates precise aliquoting, and storage at −20 °C supports routine cold-chain practices.

Typical use cases include assembling qualitative receptor-ligand engagements for pathway demonstrations, creating platform checks where a defined growth factor input is required, and building training exercises focused on handling lyophilized cytokine-like reagents. The biotechnology-grade statement (minimum 95% purity; solutions prepared with Type I ultrapure water and filtered) aligns with expectations for low background during optimization. Staying within research-only boundaries, this epigen offering provides a clearly specified, documentation-friendly component for laboratories standardizing ErbB-1–relevant exploratory work.

Why researchers choose this product:

  • CHO-expressed recombinant human epigen listed as an EGF-like growth factor
  • Supplier notes mitogenic effects on cells expressing ErbB-1
  • Lyophilized powder with pH 7.4 upon reconstitution for consistent handling
  • Specified storage at −20 °C fits routine cold-chain workflows
  • Biotechnology-grade (minimum 95%) with documentation-ready fields and keywords

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

  • 1: Verduci L, Strano S, Yarden Y, Blandino G. The circRNA-microRNA code: emerging implications for cancer diagnosis and treatment. Mol Oncol. 2019 Apr;13(4):669-680.
  • 2: Roskoski R Jr. The ErbB/HER family of protein-tyrosine kinases and cancer. Pharmacol Res. 2014 Jan;79:34-74.
  • 3: Kleibl Z, Kristensen VN. Women at high risk of breast cancer: Molecular characteristics, clinical presentation and management. Breast. 2016 Aug;28:136-44.
  • 4: Nordengen K, Kirsebom BE, Henjum K, Selnes P, Gísladóttir B, Wettergreen M, Torsetnes SB, Grøntvedt GR, Waterloo KK, Aarsland D, Nilsson LNG, Fladby T. Glial activation and inflammation along the Alzheimer’s disease continuum. J Neuroinflammation. 2019 Feb 21;16(1):46.
  • 5: Parashar D, Geethadevi A, Aure MR, Mishra J, George J, Chen C, Mishra MK, Tahiri A, Zhao W, Nair B, Lu Y, Mangala LS, Rodriguez-Aguayo C, Lopez-Berestein G, Camara AKS, Liang M, Rader JS, Ramchandran R, You M, Sood AK, Kristensen VN, Mills GB, Pradeep S, Chaluvally-Raghavan P. miRNA551b-3p Activates an Oncostatin Signaling Module for the Progression of Triple-Negative Breast Cancer. Cell Rep. 2019 Dec 24;29(13):4389-4406.e10.
  • 6: Rayego-Mateos S, Rodrigues-Diez R, Morgado-Pascual JL, Valentijn F, Valdivielso JM, Goldschmeding R, Ruiz-Ortega M. Role of Epidermal Growth Factor Receptor (EGFR) and Its Ligands in Kidney Inflammation and Damage. Mediators Inflamm. 2018 Dec 23;2018:8739473.
  • 7: Lautrup S, Caponio D, Cheung HH, Piccoli C, Stevnsner T, Chan WY, Fang EF. Studying Werner syndrome to elucidate mechanisms and therapeutics of human aging and age-related diseases. Biogerontology. 2019 Jun;20(3):255-269.
  • 8: Verduci L, Ferraiuolo M, Sacconi A, Ganci F, Vitale J, Colombo T, Paci P, Strano S, Macino G, Rajewsky N, Blandino G. The oncogenic role of circPVT1 in head and neck squamous cell carcinoma is mediated through the mutant p53/YAP/TEAD transcription-competent complex. Genome Biol. 2017 Dec 20;18(1):237.
  • 9: Pellecchia S, Sepe R, Federico A, Cuomo M, Credendino SC, Pisapia P, Bellevicine C, Nicolau-Neto P, Severo Ramundo M, Crescenzi E, De Vita G, Terracciano LM, Chiariotti L, Fusco A, Pallante P. The Metallophosphoesterase-Domain-Containing Protein 2 ( MPPED2 ) Gene Acts as Tumor Suppressor in Breast Cancer. Cancers (Basel). 2019 Jun 8;11(6):797.

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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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