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Citrate Synthase from Porcine Heart Proteins Molecular Depot
Citrate Synthase from Porcine Heart Proteins Molecular Depot
Citrate Synthase from Porcine Heart Proteins Molecular Depot
Citrate Synthase from Porcine Heart Proteins Molecular Depot

Citrate Synthase from Porcine Heart

$647.00

    Catalog Number: B2013107 (200 U)

    Citrate Synthase from Porcine Heart consists of a native Citrate Synthase, purified from Porcine Heart. This enzyme catalyzes the conversion of citrate to acetyl-CoA in the presence of coenzyme-A. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Citrate Synthase from Porcine Heart

Catalog # B2013107
Lot # Batch dependent
Expiration Batch dependent
Amount 200 U
Molecular Weight 85 kDa
Supplied as Lyophilized powder
Storage –20°C
Keywords Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)
Grade Biotechnology grade; ultrapure water (resistivity >18 MΩ·cm); filtered through 0.22 µm

Citrate Synthase from porcine heart is a key mitochondrial enzyme that catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate, the first step of the citric acid cycle. Supplied as a lyophilized powder, this biotechnology-grade preparation is highly pure and suitable for a wide range of biochemical applications including metabolic pathway analysis, enzyme kinetics, and mitochondrial function studies.

With a molecular weight of 85 kDa and an activity of 200 U per vial, this enzyme is ideal for researchers studying energy metabolism, allosteric regulation, and substrate analog inhibition. The product is stable at –20°C and should be reconstituted in sterile buffer for optimal performance. All solutions are prepared using Type I ultrapure water and filtered through 0.22 µm membranes to ensure consistency and reproducibility.

Key benefits:

  • Highly pure lyophilized enzyme from porcine heart
  • Supports studies in metabolic regulation and mitochondrial biochemistry
  • Validated activity (200 U) and molecular weight (85 kDa)
  • Stable at –20°C; batch-dependent lot and expiration
  • Filtered and formulated with ultrapure water

Indication for Use:
This product is for Research Use Only (RUO). It is not intended for human or animal use, diagnostic procedures, or therapeutic applications.

References:

  • McEvily AJ, Harrison JH. Subunit equilibria of porcine heart citrate synthase. J Biol Chem. 1986;261(6):2593–8.
  • Yoshino M et al. Activation by spermine. Biochim Biophys Acta. 1991;1073(1):200–2.
  • Bloxham DP et al. Primary structure of porcine citrate synthase. PNAS. 1981;78(9):5381–5.
  • Lee S et al. Drosophila citrate synthase characterization. Mol Cells. 1997;7(5):599–604.
  • Larson SB et al. Structure at 1.78 Å resolution. Acta Crystallogr F. 2009;65(Pt 5):430–4.
  • Johansson CJ et al. Kinetics of pig heart citrate synthase. Biochim Biophys Acta. 1973;309(2):466–72.
  • Evans CT et al. Mutant TMK368K characterization. Biochem Biophys Res Commun. 1988;157(3):1231–8.
  • Johansson CJ, Pettersson G. Substrate analogue inhibition. Eur J Biochem. 1974;46(1):5–11.
  • Johnson JK, Srivastava DK. Ligand interaction and catalysis. Arch Biochem Biophys. 1991;287(2):250–6.
  • Salvarrey MS et al. Inhibition by adenine nucleotides. Comp Biochem Physiol B. 1982;72(1):165–8.

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Product Resources:

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Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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