NativePro Bradford Protein Assay Kit (Automated)

Bradford vs. BCA Protein Assay — Why NativePro™ Wins
No Heating Required NativePro™ runs at room temperature in under 10 minutes. BCA assays typically require incubation at 37°C for 30+ minutes.

• Fully Automation-Compatible Works seamlessly with Beckman Coulter AU analyzers, microplate readers, and manual cuvettes. BCA kits often require manual prep or heating steps that limit automation.
• Ready-to-Use Reagents & Standards Includes liquid-stable R1 and R2 reagents plus six certified BSA standards — no dilution or reconstitution needed. Most BCA kits require manual formulation.
• Wide Dynamic Range Quantifies protein concentrations from 10 µg/mL to 1000 µg/mL with high precision. Comparable or better than BCA in most lab workflows.
• Detergent-Tolerant Variants Available Optional formulations compatible with common detergents — ideal for lysates and membrane proteins.
• Shelf-Stable & Lab-Ready One-year shelf life at 2–8 °C. No cold prep or special handling required.

Automatable Bradford assay with built-in standards for fast, precise protein quantitation. The NativePro Bradford Protein Assay Kit (Automated) streamlines total protein measurement by combining ready-to-use reagents with a six-level BSA calibrator set and analyzer-friendly workflows. Based on the classic Coomassie G-250 dye-binding chemistry, the assay delivers a broad analytical range of 10–1000 µg/mL with excellent linearity and low background, enabling consistent calibration curves across plates, cuvettes, and high-throughput systems. A typical run completes in ~10 minutes, minimizing turnaround time while preserving sensitivity for purified enzymes, antibodies, lysates, and other protein samples.

NativePro is engineered for flexibility. The kit is validated on common automated chemistry analyzers— including the Beckman Coulter AU family— as well as microplate handlers, and it can be executed manually when required. Preformulated R1/R2 reagents eliminate on-bench mixing and reduce lot-to-lot variability; paired with traceable BSA standards (six concentrations), the kit supports rigorous QC and inter-experiment comparability. Whether you are quantifying yields during purification, normalizing samples prior to electrophoresis, or performing routine QC checks, NativePro brings repeatable performance to RUO labs that need speed without sacrificing accuracy.

The included documentation details analyzer setup and plate protocols to accelerate adoption. For best results, equilibrate reagents to room temperature, follow analyzer-specific aspiration/dispense parameters, and run the full standard set to generate a robust curve across the 10–1000 µg/mL range. With its combination of speed, compatibility, and traceability, NativePro is a powerful upgrade over ad-hoc Bradford mixes and manual standard preparation.

Why researchers choose this kit:

• Broad 10–1000 µg/mL dynamic range with excellent linearity
• Analyzer-ready: works on major automated chemistry systems and plate handlers
• ~10-minute protocol reduces turnaround and increases throughput
• Six traceable BSA standards included for accurate curves and QC
• Preformulated R1/R2 reagents reduce prep steps and variability

Indication for Use:
This kit is for Research Use Only (RUO). It is not intended for diagnostic procedures or therapeutic applications.

Brochure / Datasheet:
Download PDF

NativePro Performance Data

Calibration Curve

NativePro Precision

NativePro Method Comparison

NativePro Dilution Linearity

References

• Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248-54.
• Schleicher E, Wieland OH. Evaluation of the Bradford method for protein determination in body fluids. J Clin Chem Clin Biochem. 1978 Sep;16(9):533-4.
• Sharma SK, Babitch JA. Application of Bradford’s protein assay to chick brain subcellular fractions. J Biochem Biophys Methods. 1980 Apr;2(4):247-50.
• Friedenauer S1, Berlet HH. Sensitivity and variability of the Bradford protein assay in the presence of detergents. Anal Biochem. 1989 May 1;178(2):263-8.
• Ernst O1, Zor T. Linearization of the bradford protein assay. J Vis Exp. 2010 Apr 12;(38). pii: 1918.
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• Ammar A, Trabelsi K, Müller P, Bouaziz B, Boukhris O, Glenn JM, Bott N, Driss T, Chtourou H, Müller N, Hökelmann A. The Effect of (Poly)phenol-Rich Interventions on Cognitive Functions and Neuroprotective Measures in Healthy Aging Adults: A Systematic Review and Meta-Analysis. Clin Med . 2020 Mar 19;9(3). pii: E835.