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GFP-Agarose Resin Molecular Biology Molecular Depot
GFP-Agarose Resin Molecular Biology Molecular Depot
GFP-Agarose Resin Molecular Biology Molecular Depot
GFP-Agarose Resin Molecular Biology Molecular Depot

GFP-Agarose Resin

$2,195.00

    Catalog Number: B2017220 (20 rxns)

    GFP-Agarose Resin is a high quality type of affinity chromatography resin used for the purification of proteins that are tagged with Green Fluorescent Protein (GFP). GFP is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range, and it is commonly used as a molecular tag. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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GFP-Agarose Resin – Catalog #B2017220 (20 rxns)

Catalog # B2017220
Lot # Batch dependent
Expiration Batch dependent
Amount 20 rxns
Supplied As Solution (agarose resin slurry)
Applications Affinity purification of GFP-tagged proteins
Storage 2–8 °C
Keywords GFP tag, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, AcGFP, sfGFP, TagGFP, Citrine, Venus, Ypet
Grade Biotechnology grade; highly pure; made with Type I ultrapure water >18 MΩ·cm, filtered through 0.22 µm

GFP-Agarose Resin is a specialized affinity chromatography matrix engineered for fast, selective capture and purification of GFP-tagged proteins from complex lysates. Designed for high-specificity pull-downs and preparative enrichment, this agarose-based resin binds Green Fluorescent Protein (GFP) and common variants (eGFP, YFP/eYFP, BFP, CFP, sfGFP, TagGFP, Citrine, Venus, YPet), enabling clean isolation of fusion proteins for downstream biochemical analysis, proteomics, interaction mapping, and enzyme activity assays. Packaged as a ready-to-use slurry (20 reactions; Catalog #B2017220), the resin integrates seamlessly into gravity-flow columns, spin devices, and batch-binding formats used in molecular biology and protein science workflows.

Optimized coupling chemistry and controlled ligand density deliver strong affinity with minimal nonspecific interactions, supporting high purity and excellent recovery even from challenging sample matrices. Researchers can rapidly perform immunoprecipitation-style pull-downs of GFP fusion constructs, co-purify binding partners for complex analysis, or quickly polish partially purified material prior to structural or functional studies. The biotechnology-grade preparation—manufactured with ultrapure water and fine filtration—ensures consistent performance and reproducibility across lots, while 2–8 °C storage maintains resin stability over multiple uses. Whether you are screening construct expression, validating protein-protein interactions, or preparing material for mass spectrometry, this GFP affinity resin streamlines experimental setup and accelerates time-to-result.

Typical applications include: rapid enrichment of GFP-tagged proteins for SDS-PAGE/Western blot, gentle isolation for activity assays, and capture-and-release protocols compatible with native conditions. The slurry format simplifies packing and scaling from analytical to preparative volumes, and bulk options are available for high-throughput projects. With robust selectivity, low background, and user-friendly handling, GFP-Agarose Resin is a dependable workhorse for laboratories standardizing GFP-fusion protein purification and interaction studies.

Why researchers choose this product:

  • High-affinity, low-background capture of GFP and common GFP variants
  • Works with gravity columns, spin protocols, and batch incubation
  • Maintains protein functionality for native and activity-based assays
  • Biotech-grade preparation ensures reproducibility and scalability
  • Convenient slurry format with bulk options for high-throughput labs

Indication for Use:
This product is intended for Research Use Only (RUO)

References

  • Voytas D. Agarose gel electrophoresis Curr Protoc Mol Biol. 2001 May;Chapter 2:Unit2.5A.
  • Horinaka JI, Ogawa S. Cyclic deformation behavior of agarose hydrogels prepared at different gelation concentrations Int J Biol Macromol. 2023 Sep 1;248:125904.
  • Rajendran V, Jain MV. In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells Methods Mol Biol. 2018;1692:89-95.
  • Topuz F, Nadernezhad A, Caliskan OS, Menceloglu YZ, Koc B. Nanosilicate embedded agarose hydrogels with improved bioactivity Carbohydr Polym. 2018 Dec 1;201:105-112.
  • Upcroft P, Upcroft JA. Comparison of properties of agarose for electrophoresis of DNA J Chromatogr. 1993 Aug 25;618(1-2):79-93.
  • Yu S, Park SY, Kim DH, Yun EJ, Kim KH. Multi-Step Enzymatic Production and Purification of 2-Keto-3-Deoxy-Galactonate from Red-Macroalgae-Derived Agarose Mar Drugs. 2022 Apr 25;20(5):288.
  • Duro G, Izzo V, Barbieri R. Methods for recovering nucleic acid fragments from agarose gels J Chromatogr. 1993 Aug 25;618(1-2):95-104.
  • Stellwagen NC, Stellwagen J. “Flip-flop” orientation of agarose gel fibers in pulsed alternating electric fields Electrophoresis. 1993 Apr;14(4):355-68.
  • Cao L, Zhu J, Li N. Selenium-agarose hybrid hydrogel as a recyclable natural substrate for selenium-enriched cultivation of mung bean sprouts Int J Biol Macromol. 2022 Jan 1;194:17-23.

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

    Purchase Orders
    Confirmed orders are final and may not be canceled or refunded unless authorized in writing.

    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

    Safety Guidelines
    Always consult the product’s SDS. Products must not be ingested, inhaled, or come into contact with skin or eyes unless specified.

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    Changes or Cancellations
    All changes must be in writing and approved. Cancellations may result in fees for materials, labor, or third-party costs.

    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

    Delivery & Claims
    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

    Extended Payment Terms
    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
    Ownership remains with Blue Tiger Scientific or Molecular Depot LLC until payment is complete. Goods must be stored separately and labeled until paid in full.

    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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