Human Proteinase 3 (PR-3) from Neutrophil Native – Catalog number: B2017055 (50 µg)

Human Proteinase 3 (PR-3) from Neutrophil Native is provided as a biotechnology-grade solution in a defined 50 µg amount (Catalog B2017055) with a listed molecular weight of 29 kDa. PR-3 is described as a serine protease found in neutrophils, a white-blood-cell lineage involved in host response processes. In alignment with the supplier’s stated use, this product is intended as a molecular tool for various biochemical applications and is stored at −80 °C. The concise specification set—amount, supplied-as, molecular weight, storage, and keyword synonyms—supports straightforward SOP documentation and inventory control for research labs standardizing on PR-3-related workflows.

In method development, a native PR-3 preparation can be incorporated into exploratory protocols that examine protease handling, binding, or detection under well-controlled conditions. The solution format allows direct dilution into user-selected buffers for pilot titrations, comparative matrix checks, or qualitative readouts where PR-3 specificity is relevant to the study design. Given that the product is presented explicitly as a research tool rather than a ready-qualified assay component, best practice is to establish working conditions empirically—documenting buffer composition and temperature history—so results are reproducible across instruments and operators. The biotechnology-grade note (Type I ultrapure water; 0.22 µm filtration for solutions) aligns with expectations for low background in bench-scale biochemical applications.

Typical research scenarios may include constructing proof-of-concept capture or detection elements, evaluating sample-prep steps that influence protease behavior, or establishing internal references for qualitative comparisons across experimental runs. The clear storage guidance (−80 °C) supports long-term stability planning and encourages aliquoting to minimize freeze–thaw. By pairing precisely stated specifications with a ready-to-use format, this PR-3 offering provides a practical option for laboratories that need a native neutrophil serine protease as part of broader biochemical investigations.

Why researchers choose this product:

• Defined 50 µg amount with listed 29 kDa molecular weight for precise documentation
• Ready-to-use solution format simplifies pilot titrations and method setup
• Stored at −80 °C per specification to support material integrity
• Biotechnology-grade preparation (Type I water; 0.22 µm filtration for solutions)
• Multiple listed synonyms (e.g., PR-3, myeloblastin) aid searchability and SOP alignment

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

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• 2: Kitamura M, Dasgupta A, Henricks J, Parikh SV, Nadasdy T, Clark E, Bazan JA, Satoskar AA. Clinicopathological differences between Bartonella and other bacterial endocarditis-related glomerulonephritis – our experience and a pooled analysis Front Nephrol. 2024 Jan 15;3:1322741.
• 3: Rao NV, Wehner NG, Marshall BC, Gray WR, Gray BH, Hoidal JR. Characterization of proteinase-3 (PR-3), a neutrophil serine proteinase. Structural and functional properties J Biol Chem. 1991 May 25;266(15):9540-8.
• 4: Fu Z, Thorpe M, Akula S, Chahal G, Hellman LT. Extended Cleavage Specificity of Human Neutrophil Elastase, Human Proteinase 3, and Their Distant Ortholog Clawed Frog PR3-Three Elastases With Similar Primary but Different Extended Specificities and Stability Front Immunol. 2018 Oct 16;9:2387.
• 5: Kao RC, Wehner NG, Skubitz KM, Gray BH, Hoidal JR. Proteinase 3. A distinct human polymorphonuclear leukocyte proteinase that produces emphysema in hamsters J Clin Invest. 1988 Dec;82(6):1963-73.
• 6: Mayet WJ, Schwarting A, Meyer zum Büschenfelde KH. Cytotoxic effects of antibodies to proteinase 3 (C-ANCA) on human endothelial cells Clin Exp Immunol. 1994 Sep;97(3):458-65.
• 7: Mayet WJ, Schwarting A, Orth T, Sibelius U, Hattar K, Meyer zum Büschenfelde KH. Signal transduction pathways of membrane expression of proteinase 3 (PR-3) in human endothelial cells Eur J Clin Invest. 1997 Nov;27(11):893-9.
• 8: Rao NV, Wehner NG, Marshall BC, Sturrock AB, Huecksteadt TP, Rao GV, Gray BH, Hoidal JR. Proteinase-3 (PR-3): a polymorphonuclear leukocyte serine proteinase Ann N Y Acad Sci. 1991;624:60-8.
• 9: Armstrong L, Godinho SI, Uppington KM, Whittington HA, Millar AB. Contribution of TNF-alpha converting enzyme and proteinase-3 to TNF-alpha processing in human alveolar macrophages Am J Respir Cell Mol Biol. 2006 Feb;34(2):219-25.
• 10: Mayet WJ, Csernok E, Szymkowiak C, Gross WL, Meyer zum Büschenfelde KH. Human endothelial cells express proteinase 3, the target antigen of anticytoplasmic antibodies in Wegener’s granulomatosis Blood. 1993 Aug 15;82(4):1221-9. pubmed.ncbi.nlm.nih.gov