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Green Nuclear Counterstain for IHC Buffers & Solutions Molecular Depot
Green Nuclear Counterstain for IHC Buffers & Solutions Molecular Depot
Green Nuclear Counterstain for IHC Buffers & Solutions Molecular Depot
Green Nuclear Counterstain for IHC Buffers & Solutions Molecular Depot

Green Nuclear Counterstain for IHC

$995.00

    Catalog Number: B2010900 (50 mL)

    Green Nuclear Counterstain for IHC consists of green nuclear counterstain solution containing methyl green. This product has been used as molecular tool for various immunohistochemistry applications. It has also been used in a wide array of other biochemical and immunological applications. For in vitro research use only. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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What's Included

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VR Headset

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2 Touch Controllers

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Charging Cable

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Power Adapter

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Green Nuclear Counterstain for IHC

Catalog number: B2010900
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mL
Molecular Weight or Concentration: na
Supplied as: Solution
Applications: molecular tool for various immunohistochemistry applications
Storage: -20°C
Keywords: Contrast Green Solution
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

Green Nuclear Counterstain for IHC is a biotechnology-grade reagent designed to enhance contrast and clarity in immunohistochemistry workflows. This counterstain selectively binds to nuclear material, providing a vivid green signal that complements chromogenic or fluorescent primary stains. Its formulation ensures minimal background interference and high specificity for nuclear structures, making it ideal for tissue section analysis, cell culture imaging, and multiplex staining protocols. Supplied as a 50 mL solution and filtered through 0.22 µm membranes using ultrapure water, it integrates seamlessly into automated and manual staining platforms.

Researchers in histopathology, oncology, and neurobiology rely on nuclear counterstains to delineate cellular architecture and validate target localization. This reagent offers consistent performance across a range of tissue types and fixation methods, and its compatibility with confocal and widefield microscopy makes it a versatile tool for high-resolution imaging. Stored at –20°C, it maintains long-term stability without compromising staining intensity. Whether you're optimizing a diagnostic assay or conducting basic research on nuclear morphology, this counterstain provides the precision and contrast needed for confident interpretation.

Key benefits:

  • Bright green nuclear signal for enhanced contrast in IHC applications

  • Compatible with chromogenic and fluorescent detection systems

  • Filtered through 0.22 µm membranes using ultrapure water

  • Supplied in 50 mL format for scalable use across multiple samples

  • Stored at –20°C for long-term stability and reproducibility

Products are for in vitro research use only (RUO).

References

  • Jones KH, Kniss DA. Propidium iodide as a nuclear counterstain for immunofluorescence studies on cells in culture J Histochem Cytochem. 1987 Jan;35(1):123-5.
  • Stefanović D, Stefanović M, Nikin Z. Romanowsky-Giemsa as a counterstain for immunohistochemistry: optimizing a traditional reagent Biotech Histochem. 2013 Aug;88(6):329-35.
  • Chantziantoniou N, Donnelly AD, Mukherjee M, Boon ME, Austin RM. Inception and Development of the Papanicolaou Stain Method Acta Cytol. 2017;61(4-5):266-280.
  • Schmued LC, Swanson LW, Sawchenko PE. Some fluorescent counterstains for neuroanatomical studies J Histochem Cytochem. 1982 Feb;30(2):123-8.
  • Kanamaru R, Ohzawa H, Miyato H, Yamaguchi H, Hosoya Y, Lefor AK, Sata N, Kitayama J. Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment J Vis Exp. 2018 Aug 3;(138):58201.
  • Gläser K, Wilke K, Wepf R, Biel SS. Visualizing nuclei in skin cryosections: viable options to 4’6-diamidino-2-phenylindol for confocal laser microscopy Skin Res Technol. 2008 Aug;14(3):324-6.
  • Tadrous PJ. Digital stain separation for histological images J Microsc. 2010 Nov;240(2):164-72.
  • Fei Y, Hughes TE. Nuclear trafficking of photoreceptor protein crx: the targeting sequence and pathologic implications Invest Ophthalmol Vis Sci. 2000 Sep;41(10):2849-56.
  • Burton MP, Schneider BG, Brown R, Escamilla-Ponce N, Gulley ML. Comparison of histologic stains for use in PCR analysis of microdissected, paraffin-embedded tissues Biotechniques. 1998 Jan;24(1):86-92.

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    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

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Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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