AGO3 Protein – Catalog number: B2016704 (10 µg)

AGO3 Protein (Argonaute 3) is provided as a biotechnology-grade solution in a defined 10 µg amount (Catalog B2016704) with a listed molecular weight of 100.4 kDa. As described by the supplier, AGO3 is a key component of the RNA-induced silencing complex (RISC), a central effector of RNA interference that guides sequence-specific interactions with messenger RNA. Within this framework, Argonaute family proteins load small RNAs to direct recognition of RNAs bearing complementary sequences, supporting gene-regulatory mechanisms and research into antiviral defense, developmental processes, and genome stability. The listing positions this material as a molecular tool for various biochemical applications with storage at −80 °C, enabling long-term inventory and careful, aliquot-based use.

For method development, a defined AGO3 preparation allows researchers to assemble proof-of-concept RISC-centered assays, explore binding/detection workflows that use small-RNA guides, or create qualitative references when benchmarking extraction, loading, or buffer compositions. Because it is supplied ready to dilute, teams can directly pilot working conditions in their buffers of choice to establish signal windows and background behavior. The solution format minimizes reconstitution variability; pairing that with meticulous documentation of lot identifiers, diluent composition, and time-at-temperature helps ensure reproducibility across instruments and operators. The biotechnology-grade note (solutions prepared using Type I ultrapure water and filtered through 0.22 µm) aligns with expectations for low background during bench optimization.

AGO3 has been the subject of studies examining Argonaute catalytic potential and complex assembly, including work showing human AGO3 can exhibit slicer activity under specific conditions. Such literature underscores the value of having a clearly specified AGO3 reagent when probing Argonaute behaviors, guide-dependent interactions, or related control experiments. Within research-only boundaries, this offering functions as a practical, precisely documented component for laboratories standardizing small-RNA and RNA-interference-related workflows.

Why researchers choose this product:

• Defined 10 µg fill with listed 100.4 kDa molecular weight for precise documentation
• Ready-to-dilute solution format streamlines pilot titrations and buffer screening
• Specified storage at −80 °C supports long-term stability planning
• Biotechnology-grade preparation (Type I water; 0.22 µm filtration for solutions) to reduce background
• Extensive synonym list (e.g., EIF2C3, Argonaute3) aids LIMS/SOP searchability

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

• 1: Brennecke J, Aravin AA, Stark A, Dus M, Kellis M, Sachidanandam R, Hannon GJ. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila Cell. 2007 Mar 23;128(6):1089-03.
• 2: Park MS, Phan HD, Busch F, Hinckley SH, Brackbill JA, Wysocki VH, Nakanishi K. Human Argonaute3 has slicer activity Nucleic Acids Res. 2017 Nov 16;45(20):11867-11877.
• 3: Friend K, Campbell ZT, Cooke A, Kroll-Conner P, Wickens MP, Kimble J. A conserved PUF-Ago-eEF1A complex attenuates translation elongation Nat Struct Mol Biol. 2012 Jan 8;19(2):176-83.
• 4: Qi HH, Ongusaha PP, Myllyharju J, Cheng D, Pakkanen O, Shi Y, Lee SW, Peng J, Shi Y. Prolyl 4-hydroxylation regulates Argonaute 2 stability Nature. 2008 Sep 18;455(7211):421-4.
• 5: Gunawardane LS, Saito K, Nishida KM, Miyoshi K, Kawamura Y, Nagami T, Siomi H, Siomi MC. A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila Science. 2007 Mar 16;315(5818):1587-90.
• 6: Jakymiw A, Ikeda K, Fritzler MJ, Reeves WH, Satoh M, Chan EK. Autoimmune targeting of key components of RNA interference Arthritis Res Ther. 2006;8(4):R87.
• 7: Turchinovich A, Surowy HM, Tonevitsky AG, Burwinkel B. Interference in transcription of overexpressed genes by promoter-proximal downstream sequences Sci Rep. 2016 Aug 3;6:30735.
• 8: Schürmann N, Trabuco LG, Bender C, Russell RB, Grimm D. Molecular dissection of human Argonaute proteins by DNA shuffling Nat Struct Mol Biol. 2013 Jul;20(7):818-26.
• 9: Hauptmann J, Dueck A, Harlander S, Pfaff J, Merkl R, Meister G. Turning catalytically inactive human Argonaute proteins into active slicer enzymes Nat Struct Mol Biol. 2013 Jul;20(7):814-7.
• 10: van Mierlo JT, Bronkhorst AW, Overheul GJ, Sadanandan SA, Ekström JO, Heestermans M, Hultmark D, Antoniewski C, van Rij RP. Convergent evolution of argonaute-2 slicer antagonism in two distinct insect RNA viruses PLoS Pathog. 2012;8(8):e1002872.