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Vitamin B12 Release Buffer ELISA Molecular Depot
Vitamin B12 Release Buffer ELISA Molecular Depot
Vitamin B12 Release Buffer ELISA Molecular Depot
Vitamin B12 Release Buffer ELISA Molecular Depot

Vitamin B12 Release Buffer

$795.00

    Catalog Number: B2010578 (50 mL)

    Vitamin B12 Release Buffer is an aqueous buffer for the fast and efficient release vitamin B12 from its serum/plasma transporter. Unlike other release buffers, this buffer is organic solvent-free and has been used in a wide array of biochemical and immunological applications related to vitamin B12 detection. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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Vitamin B12 Release Buffer

Catalog number: B2010578
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mL
Molecular Weight or Concentration: n/a
Supplied as: Two Liquid Solutions to combine before use
Applications: aqueous buffer for the release of vitamin B12 in homogeneous systems. Unlike other release agents, this buffer is organic solvent-free.
Storage: 2-8°C
Keywords: vitamin B12 extraction, vitamin B12 dissociation buffer, vitamin B12 release buffer, vitamin B12 releasing agent, releasing agent for vitamin B12
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

Vitamin B12 Release Buffer is a biotechnology-grade reagent designed to facilitate the efficient dissociation of vitamin B12 from protein-bound complexes in homogeneous assay systems. Supplied as two liquid components to be combined prior to use, this buffer offers a solvent-free alternative to traditional extraction methods that rely on organic reagents. Its aqueous formulation ensures compatibility with sensitive immunoassay platforms, including ELISA and CLIA, while maintaining the structural integrity of vitamin B12 for accurate quantification.

Researchers in clinical diagnostics, nutrition science, and metabolic disease studies rely on precise measurement of vitamin B12 to assess deficiency, monitor supplementation, and investigate absorption mechanisms. This buffer supports consistent analyte recovery from serum, plasma, or food matrices and integrates seamlessly into automated and manual workflows. Filtered through 0.22 µm membranes and prepared with ultrapure water, it ensures minimal background interference and high reproducibility. Stored at 2–8°C, the buffer remains stable for long-term use in assay development and validation.

Key benefits:

  • Solvent-free buffer for efficient release of vitamin B12 in homogeneous systems

  • Supplied as two liquid components for optimized activation

  • Compatible with ELISA, CLIA, and other vitamin B12 detection platforms

  • Filtered through 0.22 µm membranes using ultrapure water

  • Stable at 2–8°C for long-term use in research and assay development

Products are for in vitro research use only (RUO).

References

  • Guggisberg D, Risse MC, Hadorn R. Determination of Vitamin B12 in meat
    products by RP-HPLC after enrichment and purification on an immunoaffinity
    column. Meat Sci. 2012 Feb;90(2):279-83.

     

  • Marley EC, Mackay E, Young G. Characterisation of vitamin B12 immunoaffinity
    columns and method development for determination of vitamin B12 in a range of
    foods, juices and pharmaceutical products using immunoaffinity clean-up and high
    performance liquid chromatography with UV detection. Food Addit Contam Part A
    Chem Anal Control Expo Risk Assess. 2009 Mar;26(3):282-8.

     

  • Kundu D, Banerjee T. Carboxymethyl Cellulose-Xylan Hydrogel: Synthesis,
    Characterization, and in Vitro Release of Vitamin B12. ACS Omega.
    2019 Mar 4;4(3):4793-4803.

     

  • Heudi O, Kilinç T, Fontannaz P, Marley E. Determination of Vitamin B12 in
    food products and in premixes by reversed-phase high performance liquid
    chromatography and immunoaffinity extraction. J Chromatogr A. 2006 Jan
    6;1101(1-2):63-8.

     

  • Katz M, O’Brien R. Vitamin B12 absorption studied by vascular perfusion of
    rat intestine. J Lab Clin Med. 1979 Dec;94(6):817-25.

     

  • Adrover A, Paolicelli P, Petralito S, Di Muzio L, Trilli J, Cesa S, Tho I,
    Casadei MA. Gellan Gum/Laponite Beads for the Modified Release of Drugs:
    Experimental and Modeling Study of Gastrointestinal Release. Pharmaceutics. 2019
    Apr 17;11(4):187.

     

  • Mendes AC, Gorzelanny C, Halter N, Schneider SW, Chronakis IS. Hybrid
    electrospun chitosan-phospholipids nanofibers for transdermal drug delivery. Int
    J Pharm. 2016 Aug 20;510(1):48-56.

     

  • Santoro G, Beltrami R, Kottelat E, Blacque O, Bogdanova AY, Zobi F.
    N-Nitrosamine-{cis-Re[CO]2}(2+) cobalamin conjugates as mixed CO/NO-releasing
    molecules. Dalton Trans. 2016 Jan 28;45(4):1504-13.

     

  • Patra S, Bal DK, Ganguly S. Diffusion in and around alginate and chitosan
    films with embedded sub-millimeter voids. Mater Sci Eng C Mater Biol Appl. 2016
    Feb;59:61-69.

  • Jeter VL, Mattes TA, Beattie NR, Escalante-Semerena JC. A New Class of
    Phosphoribosyltransferases Involved in Cobamide Biosynthesis Is Found in
    Methanogenic Archaea and Cyanobacteria. Biochemistry. 2019 Feb 19;58(7):951-964.

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

    Purchase Orders
    Confirmed orders are final and may not be canceled or refunded unless authorized in writing.

    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

    Safety Guidelines
    Always consult the product’s SDS. Products must not be ingested, inhaled, or come into contact with skin or eyes unless specified.

    Legal Use Compliance
    Products must not be used in violation of any law—local, state, federal, or international.

    Accuracy of Orders
    Customers are responsible for verifying all order details before submission.

    Changes or Cancellations
    All changes must be in writing and approved. Cancellations may result in fees for materials, labor, or third-party costs.

    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

    Delivery & Claims
    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

    Extended Payment Terms
    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
    Ownership remains with Blue Tiger Scientific or Molecular Depot LLC until payment is complete. Goods must be stored separately and labeled until paid in full.

    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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