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Vitamin B12 Buffered Diluent (Liquid Stable) ELISA Molecular Depot
Vitamin B12 Buffered Diluent (Liquid Stable) ELISA Molecular Depot
Vitamin B12 Buffered Diluent (Liquid Stable) ELISA Molecular Depot
Vitamin B12 Buffered Diluent (Liquid Stable) ELISA Molecular Depot

Vitamin B12 Buffered Diluent (Liquid Stable)

$795.00

    Catalog Number: B2010583 (50 mL)

    Vitamin B12 Buffered Diluent is a liquid-stable buffered solution containing undetectable levels of vitamin B12 (< 75 pg/mL). This product has been used in the research and development of vitamin B12 high and low working solutions. This product is for research use only. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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Vitamin B12 Buffered Diluent (Liquid Stable)

Catalog number: B2010583
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 50 mL
Molecular Weight or Concentration: na
Supplied as: Liquid Solution
Applications: tool in the research and development of vitamin B12 high and low working solutions (use with High Vitamin B12 Buffered Solution, catalog number B2010582)
Storage: 2-8°C
Keywords: depleted vitamin B12 solution, vitamin B12 diluent
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

Vitamin B12 Buffered Diluent (Liquid Stable) is a biotechnology-grade reagent designed to support the preparation of vitamin B12 working solutions for assay calibration and reagent formulation. This folate-depleted matrix is optimized for use with the High Vitamin B12 Buffered Solution (Catalog # B2010582), enabling researchers to generate precise high and low concentration standards for immunoassay development, quality control, and analytical validation. Its liquid-stable format ensures ease of use and consistent performance across manual and automated workflows.

Researchers in clinical diagnostics, nutritional biochemistry, and metabolic health rely on buffered diluents to simulate physiological conditions and optimize assay sensitivity. This solution provides a clean background for accurate quantification of vitamin B12 in serum, plasma, and fortified food samples. Filtered through 0.22 µm membranes and prepared with ultrapure water, it minimizes background interference and ensures high reproducibility. Supplied in a 50 mL format and stored at 2–8°C, it integrates seamlessly into ELISA, CLIA, and other vitamin B12 detection platforms.

Key benefits:

  • Liquid-stable diluent for vitamin B12 assay calibration and reagent development

  • Designed for use with High Vitamin B12 Buffered Solution (Catalog # B2010582)

  • Provides a vitamin B12-depleted matrix for accurate quantification

  • Filtered through 0.22 µm membranes using ultrapure water

  • Supplied in 50 mL format and stored at 2–8°C for long-term stability

Products are for in vitro research use only (RUO).


References

  • 1: Andrès E, Serraj K, Zhu J, Vermorken AJ. The pathophysiology of elevated
    vitamin B12 in clinical practice. QJM. 2013 Jun;106(6):505-15.
  • 2: Zhang Y, Hodgson NW, Trivedi MS, Abdolmaleky HM, Fournier M, Cuenod M, Do KQ,
    Deth RC. Decreased Brain Levels of Vitamin B12 in Aging, Autism and
    Schizophrenia. PLoS One. 2016 Jan 22;11(1):e0146797.
  • 3: Brescoll J, Daveluy S. A review of vitamin B12 in dermatology. Am J Clin
    Dermatol. 2015 Feb;16(1):27-33.
  • 4: Baik HW, Russell RM. Vitamin B12 deficiency in the elderly. Annu Rev Nutr.
    1999;19:357-77.
  • 5: Coppen A, Bolander-Gouaille C. Treatment of depression: time to consider
    folic acid and vitamin B12. J Psychopharmacol. 2005 Jan;19(1):59-65.
  • 6: Sezer RG, Bozaykut A, Akoğlu HA, Özdemir GN. The Efficacy of Oral Vitamin B12
    Replacement for Nutritional Vitamin B12 Deficiency. J Pediatr Hematol Oncol.
    2018 Mar;40(2):e69-e72.
  • 7: Bensky MJ, Ayalon-Dangur I, Ayalon-Dangur R, Naamany E, Gafter-Gvili A, Koren
    G, Shiber S. Comparison of sublingual vs. intramuscular administration of
    vitamin B12 for the treatment of patients with vitamin B12 deficiency. Drug
    Deliv Transl Res. 2019 Jun;9(3):625-630.
  • 8: Watanabe F, Yabuta Y, Tanioka Y, Bito T. Biologically active vitamin B12
    compounds in foods for preventing deficiency among vegetarians and elderly
    subjects. J Agric Food Chem. 2013 Jul 17;61(28):6769-75.
  • 9: Arendt JFH, Horváth-Puhó E, Sørensen HT, Nexø E, Pedersen L, Ording AG,
    Henderson VW. Plasma Vitamin B12 Levels, High-Dose Vitamin B12 Treatment, and
    Risk of Dementia. J Alzheimers Dis. 2021;79(4):1601-1612.
    10: Bahadir A, Reis PG, Erduran E. Oral vitamin B12 treatment is effective for
    children with nutritional vitamin B12 deficiency. J Paediatr Child Health. 2014
    Sep;50(9):721-5.

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

    Purchase Orders
    Confirmed orders are final and may not be canceled or refunded unless authorized in writing.

    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

    Returns & Refunds
    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

    Safety Guidelines
    Always consult the product’s SDS. Products must not be ingested, inhaled, or come into contact with skin or eyes unless specified.

    Legal Use Compliance
    Products must not be used in violation of any law—local, state, federal, or international.

    Accuracy of Orders
    Customers are responsible for verifying all order details before submission.

    Changes or Cancellations
    All changes must be in writing and approved. Cancellations may result in fees for materials, labor, or third-party costs.

    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

    Delivery & Claims
    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

    Extended Payment Terms
    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
    Ownership remains with Blue Tiger Scientific or Molecular Depot LLC until payment is complete. Goods must be stored separately and labeled until paid in full.

    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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