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T4 Polynucleotide Kinase Reaction Buffer Chemicals Molecular Depot
T4 Polynucleotide Kinase Reaction Buffer Chemicals Molecular Depot
T4 Polynucleotide Kinase Reaction Buffer Chemicals Molecular Depot
T4 Polynucleotide Kinase Reaction Buffer Chemicals Molecular Depot

T4 Polynucleotide Kinase Reaction Buffer

$1,195.00

    Catalog Number: B2019317 (4 mL)

    T4 Polynucleotide Kinase Reaction Buffer is a buffer used in molecular biology for the phosphorylation of nucleic acids. It provides optimal conditions for T4 polynucleotide kinase activity, facilitating the transfer of a phosphate group from ATP to the 5′ end of nucleic acid molecules. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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VR Headset

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2 Touch Controllers

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T4 Polynucleotide Kinase Reaction Buffer

Catalog number: B2019317
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 4 mL
Molecular Weight or Concentration: N/A
Supplied as: Solution
Applications: a molecular tool for various biochemical applications
Storage: -20°C
Keywords: T4 Polynucleotide Kinase Reaction Buffer
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References

  • Chai Y, Cheng X, Xu G, Wei F, Bao J, Mei J, Ren D, Hu Q, Cen Y. A nanoplatform based on metal-organic frameworks and coupled exonuclease reaction for the fluorimetric determination of T4 polynucleotide kinase activity and inhibition Mikrochim Acta. 2020 Mar 23;187(4):243.
  • Zhang H, Zhao Z, Lei Z, Wang Z. Sensitive Detection of Polynucleotide Kinase Activity by Paper-Based Fluorescence Assay with λ Exonuclease Assistance Anal Chem. 2016 Dec 6;88(23):11358-11363.
  • Cui L, Hu J, Li CC, Wang CM, Zhang CY. An electrochemical biosensor based on the enhanced quasi-reversible redox signal of prussian blue generated by self-sacrificial label of iron metal-organic framework Biosens Bioelectron. 2018 Dec 30;122:168-174.
  • Holden NS, Tacon CE. Principles and problems of the electrophoretic mobility shift assay J Pharmacol Toxicol Methods. 2011 Jan-Feb;63(1):7-14.
  • Folk WR. Phage T4 polynucleotide kinase Gene Amplif Anal. 1981;2:299-311.
  • Tao J, Liu Z, Zhu Z, Zhang Y, Wang H, Pang P, Yang C, Yang W. Electrochemical detection of T4 polynucleotide kinase activity based on magnetic Fe(3)O(4)@TiO(2) nanoparticles triggered by a rolling circle amplification strategy Talanta. 2022 May 1;241:123272.
  • Lim RC, Gary RK. Kinetic analysis of T4 polynucleotide kinase via isothermal titration calorimetry Arch Biochem Biophys. 2024 Jun;756:109995.
  • Wu Y, Yi J, Su A, Zhang Y, Wang H, Yang L, Yang W, Pang P. An electrochemical biosensor for T4 polynucleotide kinase activity identification according to host-guest recognition among phosphate pillar[5]arene@palladium nanoparticles@reduced graphene oxide nanocomposite and toluidine blue Mikrochim Acta. 2023 Sep 16;190(10):394.
  • Wei L, Kong X, Wang M, Zhang Y, Pan R, Cheng Y, Lv Z, Zhou J, Ming J. A label-free T4 polynucleotide kinase fluorescence sensor based on split dimeric G-quadruplex and ligation-induced dimeric G-quadruplex/thioflavin T conformation Anal Bioanal Chem. 2022 Nov;414(27):7923-7933.

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Product Resources:

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Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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