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Gel Shift Binding Buffer 5X Solution Buffers & Solutions Molecular Depot
Gel Shift Binding Buffer 5X Solution Buffers & Solutions Molecular Depot
Gel Shift Binding Buffer 5X Solution Buffers & Solutions Molecular Depot
Gel Shift Binding Buffer 5X Solution Buffers & Solutions Molecular Depot

Gel Shift Binding Buffer 5X Solution

$450.00

    Catalog Number: B2010103 (4 x 500 uL)

    Gel Shift Binding Buffer 5X is a highly optimized, reproducible, buffer for use in Gel Electrophoresis Mobility Shift Assay (EMSA) and similar applications. Its proprietary composition counteracts non-specific interactions and results in sharp EMSA gel profiles. This product is supplied as a clear solution aliquoted in four vials, 500 uL each. Custom bulk orders are available upon request.

    Products are for in vitro research use only (RUO).

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Gel Shift Binding Buffer 5X Solution

Catalog number: B2010103
Lot number: Batch Dependent
Expiration date: Batch Dependent
Volume/Weight 4 x 500 uL
pH 7.5
Supplied as 5X concentrate
Appearance Clear solution
Applications Binding buffer for gel electrophoresis mobility shift assay (EMSA)
Shelf-Life 1 year from date of manufacture
Storage: Keep at -20°C
Keywords: EMSA Binding buffer, electrophoresis mobility shift assay binding buffer
Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References 1: Ream JA, Lewis LK, Lewis KA. Rapid agarose gel electrophoretic mobility shift
Related Products assay for quantitating protein: RNA interactions. Anal Biochem. 2016 Oct

Gel Shift Binding Buffer 5X Solution is a biotechnology-grade reagent formulated to support electrophoretic mobility shift assays (EMSA), a widely used technique for studying protein–nucleic acid interactions. Supplied as a 5X concentrate in four 500 µL aliquots, this buffer maintains optimal ionic strength and pH (7.5) to preserve binding specificity and complex stability during gel electrophoresis. Its clear, ready-to-use formulation ensures consistent performance across DNA, RNA, and protein-binding studies.

Researchers in molecular biology, gene regulation, and transcription factor analysis rely on EMSA buffers to detect and quantify nucleic acid–protein complexes. This buffer is designed to minimize background noise and enhance resolution of shifted bands, enabling accurate interpretation of binding events. Filtered through 0.22 µm membranes and prepared with ultrapure water, it ensures high purity and reproducibility. With a shelf life of one year and storage at –20°C, it integrates seamlessly into manual and automated workflows for gel-based interaction assays.

Key benefits:

  • 5X concentrate optimized for EMSA workflows

  • Maintains pH 7.5 for stable protein–nucleic acid interactions

  • Supplied in 4 × 500 µL aliquots for flexible use

  • Filtered through 0.22 µm membranes using ultrapure water

  • Stored at –20°C with 1-year shelf life for long-term reliability

Products are for in vitro research use only (RUO).

References

  • 1: Ream JA, Lewis LK, Lewis KA. Rapid agarose gel electrophoretic mobility shift
    assay for quantitating protein: RNA interactions. Anal Biochem. 2016 Oct
    15;511:36-41.
  • 2: Hellman LM, Fried MG. Electrophoretic mobility shift assay (EMSA) for
    detecting protein-nucleic acid interactions. Nat Protoc. 2007;2(8):1849-61.
  • 3: Fillebeen C, Wilkinson N, Pantopoulos K. Electrophoretic mobility shift assay
    (EMSA) for the study of RNA-protein interactions: the IRE/IRP example. J Vis
    Exp. 2014 Dec 3;(94):52230.
  • 4: Ramaswami S, Hayden MS. Electrophoretic mobility shift assay analysis of NF-
    κB DNA binding. Methods Mol Biol. 2015;1280:3-13.
  • 5: Buratowski S, Chodosh LA. Mobility shift DNA-binding assay using gel
    electrophoresis. Curr Protoc Pharmacol. 2001 Aug;Chapter 6:Unit6.8.
  • 6: Künne T, Westra ER, Brouns SJ. Electrophoretic Mobility Shift Assay of DNA
    and CRISPR-Cas Ribonucleoprotein Complexes. Methods Mol Biol. 2015;1311:171-84.
  • 7: Csorba T, Burgyán J. Gel mobility shift assays for RNA binding viral RNAi
    suppressors. Methods Mol Biol. 2011;721:245-52.
  • 8: Poulin-Laprade D, Burrus V. Electrophoretic Mobility Shift Assay Using
    Radiolabeled DNA Probes. Methods Mol Biol. 2015;1334:1-15.
  • 9: Sadakane Y, Hatanaka Y. Photoaffinity electrophoretic mobility shift assay
    using photoreactive DNA bearing 3-trifluoromethyl-3-phenyldiazirine in its
    phosphate backbone. Anal Biochem. 2016 Aug 1;506:1-7.
  • 10: Carruthers NJ, Parker GC, Gratsch T, Caruso JA, Stemmer PM. Protein Mobility
    Shifts Contribute to Gel Electrophoresis Liquid Chromatography Analysis. Version
    2. J Biomol Tech. 2015 Sep;26(3):103-12.
  • 11: Memelink J. Electrophoretic mobility shift assay for the analysis of
    interactions of jasmonic acid-responsive transcription factors with DNA. Methods
    Mol Biol. 2013;1011:209-25.
  • 12: García-Solaesa V, Sanz-Lozano CS. Interactions of DNA and Proteins:
    Electrophoretic Mobility Shift Assay in Asthma. Methods Mol Biol.
    2016;1434:91-105.
  • 13: Morita T, Maki K, Aiba H. Detection of sRNA-mRNA interactions by
    electrophoretic mobility shift assay. Methods Mol Biol. 2012;905:235-44. d

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Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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