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Vitamin D BSA Conjugate Biotinylated Proteins Molecular Depot
Vitamin D BSA Conjugate Biotinylated Proteins Molecular Depot
Vitamin D BSA Conjugate Biotinylated Proteins Molecular Depot
Vitamin D BSA Conjugate Biotinylated Proteins Molecular Depot

Vitamin D BSA Conjugate Biotinylated

$647.00

    Catalog Number: B2010610 (0.1 mg)

    Vitamin D BSA Conjugate Biotinylated (0.1 mg) is a concentrated solution of Bovine Serum Albumin (BSA) conjugated to both 25-OH Vitamin D3 and Biotin. A carbon spacer arm links BSA to the 25-OH Vitamin D3 moiety. Each BSA molecule contains several 25-OH Vitamin D3 units and several biotin units. Dilutions of the provided conjugate can be made in Phosphate Buffer Saline. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Vitamin D BSA Conjugate Biotinylated – Catalog number: B2010610 (0.1 mg)

Catalog number: B2010610
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 0.1 mg
Molecular Weight or Concentration: 66 kDa
Supplied as: Solution
Applications: versatile molecular tool in the study of the metabolism of vitamin D as well as the development of vitamin D assays
Storage: -20°C
Keywords: BSA-Vitamin D Biotin, Vitamin D-BSA Biotin, BSA conjugated to 25-OH Vitamin D, Vitamin D Bovine Serum Albumin Conjugate, 25-OH Vitamin D [BSA] Biotin
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

Vitamin D BSA Conjugate Biotinylated is provided as a biotechnology-grade solution in a defined 0.1 mg amount (Catalog number B2010610) with a listed molecular weight of 66 kDa. The conjugate contains bovine serum albumin (BSA) linked to 25-OH Vitamin D3 via a carbon spacer arm and additionally labeled with biotin; each BSA molecule carries several 25-OH Vitamin D3 units and several biotin units. In keeping with the supplier’s instructions, the material is stored at −20 °C and can be diluted with phosphate-buffered saline (PBS) for use in workflows.

Designed as a versatile molecular tool for research on vitamin D metabolism and for the development of vitamin D assays, this conjugate supports common streptavidin/avidin capture or detection schemes while presenting multiple 25-OH Vitamin D3 epitopes on a single carrier. The combination of a defined protein backbone, spacer-mediated presentation of the vitamin D moiety, and biotin labeling enables straightforward assembly of competitive or sandwich-style assay components, immobilization on streptavidin-coated surfaces, or inclusion as a tracer/capture element in method development. The solution format avoids reconstitution steps and simplifies titration during optimization.

Researchers constructing vitamin D detection methods can incorporate this reagent to evaluate binding conditions, calibrate capture/trace pairings, and streamline prototype CLIA-style readouts. The biotech-grade preparation (Type I ultrapure water; 0.22 µm filtration for solutions) aligns with expectations for low background and lot-to-lot reliability. Clear specifications—amount, molecular weight, storage, and supplied-as—facilitate SOP documentation and cross-lab standardization.

Why researchers choose this product:

  • Biotin-labeled BSA carrying multiple 25-OH Vitamin D3 units; spacer-linked presentation
  • Defined 0.1 mg amount and listed 66 kDa molecular weight for predictable setup
  • Ready-to-use solution; can be diluted in PBS for assay development
  • Stored at −20 °C to support stability and reproducibility
  • Appropriate as a versatile molecular tool for vitamin D metabolism research and assay construction

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

  • 1: Rozmus D, Ciesielska A, Płomiński J, Grzybowski R, Fiedorowicz E, Kordulewska N, Savelkoul H, Kostyra E, Cieślińska A. Vitamin D Binding Protein (VDBP) and Its Gene Polymorphisms-The Risk of Malignant Tumors and Other Diseases. Int J Mol Sci. 2020 Oct 22;21(21):7822.
  • 2: Lisowska-Myjak B, Jóźwiak-Kisielewska A, Łukaszkiewicz J, Skarżyńska E. Vitamin D-binding protein as a biomarker to confirm specific clinical diagnoses. Expert Rev Mol Diagn. 2020 Jan;20(1):49-56.
  • 3: Gauzzi MC. Vitamin D-binding protein and multiple sclerosis: Evidence, controversies, and needs. Mult Scler. 2018 Oct;24(12):1526-1535.
  • 4: Speeckaert MM, De Buyzere ML, Delanghe JR. Vitamin D binding protein polymorphism and COVID-19. J Med Virol. 2021 Feb;93(2):705-707.
  • 5: Davey RX. Vitamin D-binding protein as it is understood in 2016: is it a critical key with which to help to solve the calcitriol conundrum? Ann Clin Biochem. 2017 Mar;54(2):199-208.
  • 6: Jassil NK, Sharma A, Bikle D, Wang X. VITAMIN D BINDING PROTEIN AND 25-HYDROXYVITAMIN D LEVELS: EMERGING CLINICAL APPLICATIONS. Endocr Pract. 2017 May;23(5):605-613.
  • 7: Xie Z, Wang X, Bikle DD. Editorial: Vitamin D Binding Protein, Total and Free Vitamin D Levels in Different Physiological and Pathophysiological Conditions. Front Endocrinol (Lausanne). 2020 Feb 11;11:40.
  • 8: Denburg MR, Bhan I. Vitamin D-Binding Protein in Health and Chronic Kidney Disease. Semin Dial. 2015 Nov-Dec;28(6):636-44.
  • 9: Dimova R, Chakarova N, Kirilov G, Grozeva G, Shinkov A, Tankova T. Vitamin D binding protein is related to cardiac autonomic function and metabolic status in prediabetes. Nutr Res. 2020 Mar;75:56-66.
  • 10: Qiu F, Zhang L, Wang J, Li R, Yang L. Vitamin D binding protein gene polymorphisms in ulcerative colitis among those of Han Chinese ancestry: Pathogenetic, diagnostic and management implications. Asia Pac J Clin Nutr. 2020;29(3):657-666. pubmed.ncbi.nlm.nih.gov

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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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