Type | Ready-to-use 2X master mix |
Components | Accuris Hot Start Taq DNA Polymerase, 5X buffer with MgCl2, proprietary enhancers, inert red loading dye (for Master Mix Red) |
Volume | 5 ml (200 reactions, 50 µl each) |
Sensitivity | High sensitivity for low copy number PCR |
Multiplex PCR | Efficient amplification of GC-rich and AT-rich sequences |
Cycling Speed | Achieve early Ct values with rapid cycling |
Storage | Store at -20°C; avoid excessive freeze/thaw cycles |
Usage | Routine PCR, genotyping, colony PCR, fast PCR |
Compatibility | Works with a wide range of DNA templates, including GC-rich and AT-rich sequences |
Cycling Conditions | Initial denaturation: 95°C for 1 minute; 25-40 cycles: 95°C for 15 seconds, 55°C to 67°C for 15 seconds, 72°C for 15-30 seconds per kb of target DNA; Final extension: 72°C for 5 minutes |