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SDS Page Destaining Solution Buffers & Solutions Molecular Depot
SDS Page Destaining Solution Buffers & Solutions Molecular Depot
SDS Page Destaining Solution Buffers & Solutions Molecular Depot
SDS Page Destaining Solution Buffers & Solutions Molecular Depot

SDS Page Destaining Solution

$452.00

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SDS Page Destaining Solution – Catalog Number: B2010020B (100 mL)

Catalog number: B2010020B
Lot number: Batch Dependent
Expiration date: Batch dependent
Volume: 100 mL
Content Methanol and acetic acid solution
Staining Time: 1 hour to overnight
Appearance: Clear Solution
Application: SDS PAGE gels destaining
Shelf-Life: Two years from date of manufacture
Storage: Room temperature
Keywords: Coomassie blue destsaining buffer, gel destaining buffer, SDS-PAGE destaining reagent, coomassie blue destaining, coomassie destain, SDS Page destaining Solution
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

 

The SDS-PAGE Destaining Solution is a high-purity methanol/acetic acid blend designed specifically for effective removal of Coomassie blue stain from SDS-PAGE gels. Supplied as a 100 mL clear solution (Catalog B2010020B), it is stored at room temperature and boasts a shelf life of two years from manufacture. Its unparalleled clarity and graded purity ensure clean, artifact-free bands in protein electrophoresis analysis.

In protein biochemistry workflows, accurate visualization is vital. This destaining solution offers optimal contrast by rapidly washing excess dye while preserving band integrity. The recommended staining window of 1 hour to overnight offers flexibility for lab scheduling—quick enough for routine checks, yet gentle for extended protocols requiring subtle band differentiation.

The combination of methanol and acetic acid effectively shrinks the gel matrix, tightening protein complexes while flushing out background stain. This process enhances sensitivity and signal-to-noise ratio for imaging, which is critical for quantitative densitometry and downstream applications like western blotting. Labs typically inundate gels with this solution under gentle agitation, followed by visual confirmation and destaining completion.

Beyond analytical use, the solution supports method validation, image capture, and software-driven analysis. Its 99% component purity and ultrapure filtration make it compatible with automated systems and high-throughput workflows. Documentation fields like volume, storage, and grade make it ideal for SOPs and LIMS integration, ensuring consistency across experiments and users. As a staple for protein visualization, this destaining blend upholds reliability in the laboratory setting.

Why researchers choose this product:

  • Highly effective Coomassie blue removal with minimal background
  • Flexible staining time—1 hour to overnight—suits varied lab schedules
  • Clear, high-purity formulation supports precise imaging and densitometry
  • Room-temperature storage simplifies lab workflow management
  • Compatible with automated destaining systems due to biotechnology-grade clarity

This product is for Research Use Only (RUO). It is not intended for diagnostic or therapeutic use.

References

  • 1: Fernandez-Patron C, Hardy E, Sosa A, Seoane J, Castellanos L. Double staining of coomassie blue-stained polyacrylamide gels by imidazole-sodium dodecyl sulfate-zinc reverse staining: sensitive detection of coomassie blue-undetected proteins. Anal Biochem. 1995 Jan 1;224(1):263-9.
  • 2: Brum AM, Thomas AD, Sabeur K, Ball BA. Evaluation of Coomassie blue staining of the acrosome of equine and canine spermatozoa. Am J Vet Res. 2006 Feb;67(2):358-62.
  • 3: Marshall T, Williams KM. Total protein determination in urine: elimination of a differential response between the coomassie blue and pyrogallol red protein dye-binding assays. Clin Chem. 2000 Mar;46(3):392-8.
  • 4: Lim CW, Chisnall WN, Stokes YM, Pratt R, Crooke MJ. Effects of sodium dodecyl-sulphate, dye concentration and paraprotein on coomassie blue dye-binding assays for protein in urine. Clin Biochem. 1988 Oct;21(5):277-81.
  • 5: Fujita T, Toda T, Ohashi M. Enzyme-linked immunodetection of proteins on coomassie blue-stained two-dimensional cellulose acetate membranes. Anal Biochem. 1986 Nov 15;159(1):8-11.
  • 6: Fountoulakis M, Juranville JF, Manneberg M. Comparison of the Coomassie brilliant blue, bicinchoninic acid and Lowry quantitation assays, using non-glycosylated and glycosylated proteins. J Biochem Biophys Methods. 1992 Jun;24(3-4):265-74.
  • 7: Spector T. Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein. Anal Biochem. 1978 May;86(1):142-6.

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    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

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    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

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    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

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    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

    Delivery Estimates All delivery timelines are estimates and not guaranteed. FedEx service levels are subject to operational delays, weather disruptions, and regional restrictions.

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    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

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    Returns must be requested within 30 days through Blue Tiger Scientific. Refunds are only issued for shipping errors or verified defects, and processed within 45 business days if approved. Customers are responsible for return shipping costs unless otherwise stated. Refunds will exclude original shipping fees paid at checkout.

    Warranty Disclaimer
    All products are provided "as-is" with no warranties. Warranty issues are handled by the manufacturer.

    Safety Guidelines
    Always consult the product’s SDS. Products must not be ingested, inhaled, or come into contact with skin or eyes unless specified.

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    Quality System (MD Levels)
    Molecular Depot’s MD-Quality System includes:

    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

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    Orders may ship in parts. Claims for shortages or defects must be submitted within 2 days of receipt. Delivery dates are estimates only.

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    Payment is due within 30 days unless agreed otherwise. A 5% weekly penalty applies to late payments. Blue Tiger Scientific reserves discretion on payment application.

    Retention of Title
    Ownership remains with Blue Tiger Scientific or Molecular Depot LLC until payment is complete. Goods must be stored separately and labeled until paid in full.

    Intellectual Property
    No rights to manufacturing processes or IP are transferred. Blue Tiger Scientific is not liable for IP-related claims.

    Force Majeure
    Neither party is liable for delays or failures caused by uncontrollable events (e.g., weather, regulations, supply disruptions).

    Manufacturing Access
    Product purchase does not grant access to proprietary manufacturing documents or methods.

    Resale Prohibition
    Products cannot be resold without written approval from Molecular Depot LLC. Blue Tiger Scientific enforces this restriction.

    Governing Law
    Terms are governed by California and U.S. law. All disputes fall under U.S. jurisdiction.

Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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