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Pyrophosphatase, Inorganic from Baker′s Yeast (S. cerevisiae) Proteins Molecular Depot
Pyrophosphatase, Inorganic from Baker′s Yeast (S. cerevisiae) Proteins Molecular Depot

Pyrophosphatase, Inorganic from Baker′s Yeast (S. cerevisiae)

$1,395.00

    Catalog Number: B2017805 (100 Units)

    Pyrophosphatase, Inorganic from Baker’s Yeast (S. cerevisiae) catalyzes the hydrolysis of inorganic pyrophosphate into two phosphate ions. It is utilized to adjust nucleotide levels and control enzymatic reactions, vital for cellular functions like nucleic acid synthesis, energy metabolism, and phosphate balance. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.

    Products are for in vitro research use only (RUO).

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Pyrophosphatase, Inorganic from Baker′s Yeast (S. cerevisiae)

Catalog number: B2017805
Lot number: Batch Dependent
Expiration Date: Batch dependent
Amount: 100 Units
Molecular Weight or Concentration: 71 kDa
Supplied as: Powder
Applications: a molecular tool for various biochemical applications
Storage: -20°C
Keywords: Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase
Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

References

  • Christ JJ, Blank LM. Analytical polyphosphate extraction from Saccharomyces cerevisiae Anal Biochem. 2018 Dec 15;563:71-78.
  • KUNITZ M. Crystalline inorganic pyrophosphatase isolated from baker’s yeast J Gen Physiol. 1952 Jan;35(3):423-50.
  • Kolakowski LF Jr, Schloesser M, Cooperman BS. Cloning, molecular characterization and chromosome localization of the inorganic pyrophosphatase (PPA) gene from S. cerevisiae Nucleic Acids Res. 1988 Nov 25;16(22):10441-52.
  • Pérez-Castiñeira JR, Serrano A. The H(+)-Translocating Inorganic Pyrophosphatase From Arabidopsis thaliana Is More Sensitive to Sodium Than Its Na(+)-Translocating Counterpart From Methanosarcina mazei Front Plant Sci. 2020 Aug 12;11:1240.
  • Uribe S, Rangel P, Pardo JP, Pereira-Da-Silva L. Interactions of calcium and magnesium with the mitochondrial inorganic pyrophosphatase from Saccharomyces cerevisiae Eur J Biochem. 1993 Oct 15;217(2):657-60.
  • Svyato IE, Nazarova TI, Sklyankina VA, Avaeva SM. Half-of-the-sites reactivity of inorganic pyrophosphatase from yeast is the result of induced asymmetry FEBS Lett. 1984 Feb 27;167(2):269-72.
  • Kang CB, Ho KK. Characterization of a soluble inorganic pyrophosphatase from Microcystis aeruginosa and preparation of its antibody Arch Biochem Biophys. 1991 Sep;289(2):281-8.
  • Halonen P, Baykov AA, Goldman A, Lahti R, Cooperman BS. Single-turnover kinetics of Saccharomyces cerevisiae inorganic pyrophosphatase Biochemistry. 2002 Oct 8;41(40):12025-31.
  • Kasho VN, Avaeva SM. Isolation of the inorganic pyrophosphatase from brewer’s yeast and studies on the localization of this enzyme in brewer’s and baker’s yeast Int J Biochem. 1978;9(1):51-6.

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Product Resources:

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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