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NativeLyser Soluble Protein Lysis Buffer
NativeLyser Soluble Protein Lysis Buffer
NativeLyser Soluble Protein Lysis Buffer
NativeLyser Soluble Protein Lysis Buffer
NativeLyser Soluble Protein Lysis Buffer
NativeLyser Soluble Protein Lysis Buffer

NativeLyser Soluble Protein Lysis Buffer

$550.00

    Catalog #: B2010007 (1 L)


    NativeLyser is a bacterial cell lysis buffer formulated to promote the efficient breakdown of bacterial cells while solubilizing and stabilizing the released recombinant proteins. NativeLyser contains a proprietary mix of stabilizers that prevent the loss of recombinant proteins during the preparation of cell-free extracts. By preventing denaturation, proteolytic degradation and non-specific protein aggregation, NativeLyser creates a protein-friendly environment when recombinant proteins are released from the bacterial cytoplasm. We recommend the use of NativeLyser to purify proteins expressed using the NativeFolder Bacterial Culture Medium (Catalog number K1010004). NativeLyser is provided as a 5X concentrated solution (200 mL) that can be diluted with di-water to produce 1L of ready-to-use buffer. Custom bulk orders of this product are available upon request.

    Products are for in vitro research use only (RUO).

    Live Inquiry about this product via:

    Email: info@bluetigerscientific.com

    Text/SMS: (833) 928-4437 Mon–Fri, (8 am – 8 pm PST)

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What's Included

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VR Headset

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2 Touch Controllers

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Charging Cable

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Power Adapter

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Glasses Spacer

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NativeLyser Soluble Protein Lysis Buffer

Catalog number: B2010007
Volume: 200 mL (5X concentrate, yields 1 L)
Formulation: Proprietary stabilizer mix
Applications: Bacterial cell lysis, recombinant protein solubilization, stabilization of cell-free extracts
Recommended Pairing: NativeFolder Bacterial Culture Medium (Catalog #K1010004)
Storage: 2–8°C
Keywords: lysis buffer, recombinant protein buffer, bacterial extract buffer, soluble protein stabilization

References

  • Packiam KAR, Ramanan RN, Ooi CW, Krishnaswamy L, Tey BT. Stepwise optimization of recombinant protein production in Escherichia coli utilizing computational and experimental approaches. Appl Microbiol Biotechnol. 2020 Apr;104(8):3253–3266.
  • Satheeshkumar PK. Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update. Mol Biotechnol. 2020 Mar;62(3):151–167.
  • Smolskaya S, Logashina YA, Andreev YA. Escherichia coli Extract-Based Cell-Free Expression System as an Alternative for Difficult-to-Obtain Protein Biosynthesis. Int J Mol Sci. 2020 Jan 31;21(3). pii: E928.
  • Lipinski DM. A Comparison of Inducible Gene Expression Platforms: Implications for Recombinant Adeno-Associated Virus (rAAV) Vector-Mediated Ocular Gene Therapy. Adv Exp Med Biol. 2019;1185:79–83.
  • Powell N, Pantazi E, Pavlidis P, Tsakmaki A, Li K, Yang F, Parker A, Pin C, Cozzetto D, Minns D, Stolarczyk E, Saveljeva S, Mohamed R, Lavender P, Afzali B, Digby-Bell J, Tjir-Li T, Kaser A, Friedman J, MacDonald TT, Bewick GA, Lord GM. Interleukin-22 orchestrates a pathological endoplasmic reticulum stress response transcriptional programme in colonic epithelial cells. Gut. 2020 Mar;69(3):578–590.
  • Temporini C, Colombo R, Calleri E, Tengattini S, Rinaldi F, Massolini G. Chromatographic tools for plant-derived recombinant antibodies purification and characterization. J Pharm Biomed Anal. 2020 Feb 5;179:112920.
  • de Marco A. Recombinant expression of nanobodies and nanobody-derived immunoreagents. Protein Expr Purif. 2020 Apr 11;172:105645.
  • Knight T, Callaghan MU. The role of emicizumab, a bispecific factor IXa- and factor X-directed antibody, for the prevention of bleeding episodes in patients with hemophilia A. Ther Adv Hematol. 2018 Oct 10;9(10):319–334.
  • Bello-Rivero I, Garcia-Vega Y, Duncan-Roberts Y, Vazquez-Blomquist D, Santana-Milian H, Besada-Perez V, Rios-Cabrera M. HeberFERON, a new formulation of IFNs with improved pharmacodynamics: Perspective for cancer treatment. Semin Oncol. 2018 Jan;45(1–2):27–33.

    Terms & Conditions

    Blue Tiger Scientific is an independent third-party distributor of select products manufactured by Molecular Depot LLC. By purchasing from bluetigerscientific.com, you (“the Customer”) agree to the following terms, adapted from Molecular Depot’s original conditions:

    Product Use & Eligibility
    Products are for in vitro research use only (RUO). They are not intended for human or animal use, diagnosis, therapy, resale to consumers, or residential delivery. Sales are limited to verified business addresses.

    Payment Terms
    All purchases are prepaid and non-refundable once confirmed. Pricing is in USD and subject to change without notice.

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    Limitation of Liability
    Blue Tiger Scientific and Molecular Depot LLC are not responsible for any damages resulting from product use or misuse.

    Waiver of Claims
    By ordering, you waive all claims arising from use of Molecular Depot products.

    Shipping & Handling
    Shipping is calculated at checkout. A minimum 27% handling fee applies to each order.

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    FedEx Pickup Cutoff Orders submitted after the daily FedEx pickup window may not ship until the next available pickup day.

    Shipping Exceptions FedEx may impose restrictions or delays due to package size, weight, destination, or service availability. Blue Tiger Scientific is not liable for delays beyond our control.

    Weekend & Holiday Handling Orders placed on weekends or holidays will be processed on the next business day.

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    Quality System (MD Levels)
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    MD 1000: Non-regulated use, no change notification

    MD 2000: Non-regulated use, limited change notification

    MD 3000: Regulated use, enhanced quality standards

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Product Resources:

Manual Manual
Brochure Brochure

Copy of Technical Specifications

FeatureDetails
Viewing Head Siedentopf type trinocular head, inclined at 30°, Interpupillary adjustment 53mm to 75mm, graduated diopter on left eyetube (30mm I.D. eyetubes)
Eyepieces SWH10X Widefield high eyepoint eyepiece, Field No. 22, tube O.D. 30.0 mm
Nosepiece Quintuple
Quintuple LWD Planachromat Phase 10x, 20x
Condenser TC Condenser N.A. 0.30, W.D. 73.0mm
Stage180mm(X) x 245mm(Y) plain stage with replaceable glass insert with 45mm opening, Glass Stage plate insert
IlluminationKoehler without iris, with phase slider, 3W LED
WarrantyLIMITED LIFETIME WARRANTY

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