Metastasis-Suppressor KiSS-1 (Metastin/Kisspeptin-1) Polyclonal Antibody – Research Use Only

Metastasis-Suppressor KiSS-1 (Metastin/Kisspeptin-1) Polyclonal Antibody is a high-quality rabbit polyclonal antibody raised against the human KiSS-1 derived peptide (metastin/kisspeptin-1). Supplied as a 50 µL solution (Catalog #B2017151), this antibody is a valuable research tool for studying the KiSS-1/kisspeptin system, which plays critical roles in regulating reproduction (GnRH secretion), metastasis suppression, and neuroendocrine signaling.

Scientific Overview

The KiSS-1 gene encodes a precursor protein that is processed into kisspeptins (including kisspeptin-1/metastin), which act as potent ligands for the G-protein-coupled receptor GPR54 (KISS1R). This signaling pathway is a master regulator of gonadotropin-releasing hormone (GnRH) secretion and puberty onset, while the KiSS-1 product also functions as a metastasis suppressor in various cancers. The polyclonal antibody recognizes the KiSS-1/metastin/kisspeptin-1 system and is widely used to investigate its expression, localization, and functional roles in reproductive endocrinology, cancer biology, and neuroendocrine regulation.

This Metastasis-Suppressor KiSS-1 Polyclonal Antibody is suitable for:

• Immunohistochemical detection of kisspeptin neurons in brain and peripheral tissues
• Western blot analysis of KiSS-1 expression in normal and malignant tissues
• Studies on the kisspeptin/GPR54 axis in reproduction and puberty
• Investigation of metastasis suppression mechanisms in cancer research
• Neuroendocrine and hormonal regulation studies

Usage & Handling Guidance

Store at -20 °C. Avoid repeated freeze–thaw cycles by preparing single-use aliquots. For immunohistochemistry or immunofluorescence, dilute according to the specific protocol (typical range 1:100–1:1000). For Western blotting, optimize dilution starting from 1:500–1:2000. Centrifuge briefly before use to remove any aggregates.

• Recommended applications: IHC, Western blot, ELISA, and immunofluorescence
• Working dilution: Optimize empirically (commonly 1:100–1:2000 depending on application and sample)
• Stability: Stable when stored frozen; aliquot to prevent repeated freeze–thaw
• Handling: Use in a clean environment; validate specificity with appropriate controls

What You Get

• 50 µL Metastasis-Suppressor KiSS-1 (Metastin/Kisspeptin-1) Polyclonal Antibody solution
• Rabbit polyclonal antibody specific for the KiSS-1/kisspeptin system
• High-purity biotechnology-grade reagent suitable for multiple immunoassay techniques
• Convenient volume for multiple experiments and method optimization
• For research use only (RUO)

Why Researchers Choose It

• Specific polyclonal antibody targeting the KiSS-1/metastin/kisspeptin-1 system
• Versatile for immunohistochemistry, Western blotting, and functional studies
• Essential tool for research on reproduction, puberty, and cancer metastasis suppression
• High-quality formulation ensures reliable and reproducible results
• Well-documented applications in neuroendocrine and oncology research

Frequently Asked Questions (FAQ)

• What does this antibody recognize?
  It recognizes the KiSS-1 gene product, including metastin and kisspeptin-1 peptides.
• What are the main research applications?
  It is commonly used for IHC detection of kisspeptin neurons, Western blot analysis of KiSS-1 expression, and studies of the kisspeptin/GPR54 axis in reproduction and metastasis.
• What dilution should I start with?
  Typical starting dilutions are 1:100–1:1000 for IHC/IF and 1:500–1:2000 for Western blot. Optimize for your specific sample and protocol.
• How should I store the antibody?
  Store at -20 °C and avoid repeated freeze–thaw cycles by making aliquots.
• Is this suitable for human and animal tissues?
  Yes. It has been used successfully in both human and rodent tissues for neuroendocrine and cancer research.

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References

• Dun SL, Brailoiu GC, Parsons A, Yang J, Zeng Q, Chen X, Chang JK, Dun NJ. Metastin-like immunoreactivity in the rat medulla oblongata and spinal cord. Neurosci Lett. 2003 Jan 2;335(3):197-201.
• Muir AI, Chamberlain L, Elshourbagy NA, Michalovich D, Moore DJ, Calamari A, Szekeres PG, Sarau HM, Chambers JK, Murdock P, Steplewski K, Shabon U, Miller JE, Middleton SE, Darker JG, Larminie CG, Wilson S, Bergsma DJ, Emson P, Faull R, Philpott KL, Harrison DC. AXOR12, a novel human G protein-coupled receptor, activated by the peptide KiSS-1. J Biol Chem. 2001 Aug 3;276(31):28969-75.
• Clarkson J, d’Anglemont de Tassigny X, Colledge WH, Caraty A, Herbison AE. Distribution of kisspeptin neurones in the adult female mouse brain. J Neuroendocrinol. 2009 Aug;21(8):673-82.
• González-León K, Beltrán-Pérez G, Muñoz-Aguirre S, López-Gayou V, Castillo-Mixcoatl J, Alatriste V, Delgado-Macuil R. Experimental characterization of a biosensor based on a tapered optical fiber for kisspeptin detection. Appl Opt. 2020 May 1;59(13):D131-D137.
• Meczekalski B, Niwczyk O, Bala G, Szeliga A. Stress, kisspeptin, and functional hypothalamic amenorrhea. Curr Opin Pharmacol. 2022 Dec;67:102288.
• Ji K, Ye L, Mason MD, Jiang WG. The Kiss-1/Kiss-1R complex as a negative regulator of cell motility and cancer metastasis (Review). Int J Mol Med. 2013 Oct;32(4):747-54.
• Navarro VM. Metabolic regulation of kisspeptin – the link between energy balance and reproduction. Nat Rev Endocrinol. 2020 Aug;16(8):407-420.
• Kolioulis I, Zafrakas M, Grimbizis G, Miliaras D, Timologou A, Bontis JN, Tarlatzis BC. Immunohistochemical expression pattern of metastasis suppressor KISS-1 protein in adenomyosis lesions and normal endometrium. Eur J Obstet Gynecol Reprod Biol. 2017 Mar;210:64-68.
• Argente J, Dunkel L, Kaiser UB, Latronico AC, Lomniczi A, Soriano-Guillén L, Tena-Sempere M. Molecular basis of normal and pathological puberty: from basic mechanisms to clinical implications. Lancet Diabetes Endocrinol. 2023 Mar;11(3):203-216.